LOCUS       sequence-334718-        1478 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SCAT3.2 is fluorescent reporter (FRET probe) for cell 
            survival/apoptosis with Caspase-3 sensitive sequence which can be 
            introduced to mammalian cells by retrovirus vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Oki T, Mercier F, Kato H, Jung Y, McDonald TO, Spencer JA, Mazzola 
            MC, van Gastel N, Lin CP, Michor F, Kitamura T, Scadden DT
  TITLE     Imaging dynamic mTORC1 pathway activity in vivo reveals marked 
            shifts that support time-specific inhibitor therapy in AML.
  JOURNAL   Nat Commun. 2021 Jan 11;12(1):245. doi: 10.1038/s41467-020-20491-8.
  PUBMED    33431855
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-11"; volume: "12(1):245. doi"; pages: " 
            10.1038/s41467-020-20491-8"
COMMENT     Sequence Label: pMXs-IP- SCAT3.2
FEATURES             Location/Qualifiers
     source          1..1478
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             13..699
                     /codon_start=1
                     /product="Anemonia majano cyan fluorescent protein"
                     /label=AmCyan1
                     /note="mammalian codon-optimized"
                     /translation="MALSNKFIGDDMKMTYHMDGCVNGHYFTVKGEGSGKPYEGTQTST
                     FKVTMANGGPLAFSFDILSTVFMYGNRCFTAYPTSMPDYFKQAFPDGMSYERTFTYEDG
                     GVATASWEISLKGNCFEHKSTFHGVNFPADGPVMAKKTTGWDPSFEKMTVCDGILKGDV
                     TAFLMLQGGGNYRCQFHTSYKTKKPVTMPPNHVVEHRIARTDLDKGGNSVQLTEHAVAH
                     ITSVVPF"
     CDS             join(754..756,757..1470)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(796..817)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1057..1076)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1404..1425
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggatccgcca ccatggccct gtccaacaag ttcatcggcg acgacatgaa gatgacctac
       61 cacatggacg gctgcgtgaa cggccactac ttcaccgtga agggcgaggg cagcggcaag
      121 ccctacgagg gcacccagac ctccaccttc aaggtgacca tggccaacgg cggccccctg
      181 gccttctcct tcgacatcct gtccaccgtg ttcatgtacg gcaaccgctg cttcaccgcc
      241 taccccacca gcatgcccga ctacttcaag caggccttcc ccgacggcat gtcctacgag
      301 agaaccttca cctacgagga cggcggcgtg gccaccgcca gctgggagat cagcctgaag
      361 ggcaactgct tcgagcacaa gtccaccttc cacggcgtga acttccccgc cgacggcccc
      421 gtgatggcca agaagaccac cggctgggac ccctccttcg agaagatgac cgtgtgcgac
      481 ggcatcttga agggcgacgt gaccgccttc ctgatgctgc agggcggcgg caactacaga
      541 tgccagttcc acacctccta caagaccaag aagcccgtga ccatgccccc caaccacgtg
      601 gtggagcacc gcatcgccag aaccgacctg gacaagggcg gcaacagcgt gcagctgacc
      661 gagcacgccg tggcccacat cacctccgtg gtgcccttct cctcgtccga gctcagcgga
      721 gatgaggtcg atggtaccag cggaagcgaa ttcgtgagca agggcgagga gctgttcacc
      781 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      841 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc
      901 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggcctgcag
      961 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1021 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1081 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1141 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     1201 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac
     1261 aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1321 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa
     1381 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1441 actctcggca tggacgagct gtacaagtaa gcggccgc
//
