LOCUS       sequence-334726-       11696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 construct: actinb (actb2) promoter driven zebrafish 
            gain-of-function kcnj13 mutant, M135R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11696)
  AUTHORS   Silic MR, Wu Q, Kim BH, Golling G, Chen KH, Freitas R, Chubykin AA, 
            Mittal SK, Zhang G
  TITLE     Potassium Channel-Associated Bioelectricity of the Dermomyotome 
            Determines Fin Patterning in Zebrafish.
  JOURNAL   Genetics. 2020 Aug;215(4):1067-1084. doi: 
            10.1534/genetics.120.303390. Epub 2020 Jun 16.
  PUBMED    32546498
REFERENCE   2  (bases 1 to 11696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.120.303390"; journalName: "Genetics"; date: 
            "2020-08"; volume: "215"; issue: "4"; pages: "1067-1084"
COMMENT     Sequence Label: pDestTol2-actinb-kcnj13-M135R-EGFP
FEATURES             Location/Qualifiers
     source          1..11696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    269..293
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1419..1443)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1442..1451
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1448..1450,1451..1453,1454..2164)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1448..1450,1451..1453,1454..2164)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1493..1514)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1754..1773)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2101..2122
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2217..2236)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2218..2236)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2241..2375
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2438..2458)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2466..2482)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2498..2632
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(3071..3258,3259..3270)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3304..3320)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3304..3320)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3357..3377)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3357..3375)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3396..3412)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3409..3431)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3420..3436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3444..3450,3451..3468,3469..3474))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3489..3510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3627..3644)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3798..4386)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3878..3897)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4557..5348,5349..5417))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5180..5199
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5418..5522)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5685..5706)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(6055..6066,6067..6229)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     6602..6618
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6638..6658
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ccctacgact ctggctgagt gccacaccgc cggcagccgc aaagcggctc aaaccattgc
       61 cttttatggt aataatgaga gaatgcagag ggacttcctt tgtctggcat atctgaggcg
      121 cgcgctgtca ctagcgccca ccagcggtca gactgtagaa tgcagagcaa aacaggaagt
      181 tgactccaga tggtcacatg cttgctgaaa cgcctctccc tggcagcagt gcaattctaa
      241 cagtttccct ttcttttaca gttcagcctc aagtttgtac aaaaaagcag gctccgcggc
      301 cgcccccttc accatgccta ccaccatgac aaacaccacc gctgaccaaa aggcctcctg
      361 ccctttgatg gtcaaacctc agcgtcgccg cctggtcagc aaagacggtc ggagccagac
      421 aagaggaaac acaagaggag gctccagaga gacgtgtttc agtgctctgc gggacttgtg
      481 gggcacatgg ctggcgctac ggtggcggtg ggtggttttg gcattttgtg gctcattcct
      541 gcttcactgg ctgctgtttg ctgtattatg gtacctgctg gctcgggtca atggagacct
      601 ggatgtcctg gatcatgatt ctcctcctcc cggtcatgtg ctgtgtgtta aacatgtgaa
      661 tgggttcaca gccgcttttt cattcgccct ggagacgcaa ctcactattg ggtatggcac
      721 acggtaccca aacgcagact gccccactgc catcgcgctg cttgccctgc agatgctgct
      781 gggactcatg ctggaggcct ttatcactgg tgcatttgta gccaagttct ctcggcctca
      841 gaagcgatgt gacgggatcc tattcagccc tcaggctgtg gtgtgtgagc agaaaggcca
      901 gcgctgtcta atgtttcgag tgtgtaacct ccagccacag ccgctagtgg acgtatccgt
      961 gagtgctgtc ctctacgagg agcgagacga tcacgaactc caccaaactg ctctggagtt
     1021 cagcatcgac aacctgggct ccagatcttg ccctcttttc ctctcccctc tgaccttctt
     1081 tcacccttta aatccatcca ctcctttcat caacaaccca agcagccaaa cacactttga
     1141 gcttgtagtg tttctcactg cgactcagga aagcacaggg tctggttacc acaagaggac
     1201 gtcttacctg cctgacgaga tccaatatgg ctactgcttc tccaaggtga cgtctgtaca
     1261 ccagaacaaa accccaaaca tgcgctattt tgacacggtg ccctgtcccc tcacactagc
     1321 caacacacac accactgata caccggataa agagcatgtg gtggttcagc tgaatggaga
     1381 aggcagcgac cgcgtggaga agaagggtgg gcgcgccgac ccagctttct tgtacaaagt
     1441 ggccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     1501 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     1561 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     1621 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     1681 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     1741 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     1801 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     1861 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     1921 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     1981 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     2041 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     2101 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     2161 caagggcggt ggaagatctg ggaattcaag gcctctcgag cctctagatt ctgcagccct
     2221 atagtgagtc gtattacgta gatccagaca tgataagata cattgatgag tttggacaaa
     2281 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     2341 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     2401 tgtttcaggt tcagggggag gtgtgggagg ttttttccaa ctttattata catagttgat
     2461 aattcactgg ccgtcgtttt acggtaccat cgatgatgat ccagacatga taagatacat
     2521 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     2581 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     2641 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     2701 gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagatcaga tctgcgaaga
     2761 tacggccacg ggtgctcttg atcctgtggc tgattttgga ctgtgctgct cgcagctgct
     2821 gatgaatcac atacttcctc cattttcttc cactgattga ctgttataat ttccctaatt
     2881 tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac atgacgtcac ttccaaagga
     2941 ccaatgaaca tgtctgacca atttcatata atgtgaaaac gattttcata ggcagaataa
     3001 ataacattta aattaaactg ggcatcagcg caattcaatt ggtttggtaa tagcaaggga
     3061 aaatagaatg aagtgatctc caaaaaataa gtactttttg actgtaaata aaattgtaag
     3121 gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt
     3181 tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa
     3241 gtaaaattac tcaagtactt tacacctctg gttcttgacc ccctaccttc agcaagccca
     3301 gcagatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt
     3361 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     3421 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     3481 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     3541 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3601 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3661 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3721 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3781 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3841 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3901 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3961 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4021 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4081 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4141 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4201 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4261 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4321 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4381 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4441 cgttaaggca ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4501 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4561 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4621 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4681 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     4741 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4801 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4861 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4921 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4981 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5041 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5101 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5161 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5221 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5281 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5341 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5401 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5461 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5521 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     5581 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     5641 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     5701 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     5761 atggcccacg ctggctaaga actcatcagc ctccccggtc catctaccca cgtaccaatg
     5821 caccaattgg ccacaatgac ggctactaca tggtgccatt ccttcctctt tataggaatg
     5881 gagactacct cctgtccaac aatgctcttg gatacgagta cgcctacctg ttggacccag
     5941 gtcattgcac aacaccagaa atgccctctg atctgcaaaa gacgtgaata tctgttcaga
     6001 cacccatatc cactctgttc cacacaggtc agaggtttgt ccaggagttc ttgacagagg
     6061 tgtaaaaagt actcaaaaat tttactcaag tgaaagtaca agtacttagg gaaaatttta
     6121 ctcaattaaa agtaaaagta tctggctaga atcttacttg agtaaaagta aaaaagtact
     6181 ccattaaaat tgtacttgag tattaaggaa gtaaaagtaa aagcaagaaa gaaaactaga
     6241 gattcttgtt taagctttta atctcaaaaa acattaaatg aaatgcatac aaggttttat
     6301 cctgctttag aactgtttgt atttaattat caaactataa gacagacaat ctaatgccag
     6361 tacacgctac tcaaagttgt aaaacctcag atttaacttc agtagaagct gattctcaaa
     6421 attgttagtg tcaagcctag ctcttttggg gctgaaaagc aatcctgcag tgctgaaaag
     6481 cctctcacag gcagccgatg cgggaagagg tgtattagtc ttgatagaga ggctgcaaat
     6541 agcaggaaac gtgagcagag actccctggt gtctgaaaca caggccagat gggccctcga
     6601 gcaggaaaca gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttgaa
     6661 aattccagtt tgaagaaact tttcaagact caccttttca ctgttggtct taatttaggt
     6721 tgattatttt tgtcaattta ataattacat tgtattgttc atattttatt gtcttattgt
     6781 ggtgttttta cagctttttt attgttctgc tttgttactg tttgccttgt atgcattgta
     6841 gcgctttggg tctgagaaaa gcgcactata aataaactgt attattatta ttagggatgg
     6901 acatcacggt ggcgcgatgg gtggcacaat tacctcacag caagtaggtt gctgtttaga
     6961 gccccggctg gatcagttgg cagttctgtg tggagtttgc atgttctccc tgttcaagta
     7021 ggtttcctcc cggtgctctg gtttcccctc acaagtccaa agacatgatg tataggtgaa
     7081 tggggtaagc taaattgacc atagtgagtg tgtgtgtgtg tgtgaatgag agtgtatagg
     7141 tgtttcccag tgttaggttg caactggaag gacatctgct gtgtaaaaca tatgcaggat
     7201 aagttggcgg ttcattcccc tatggtgacc cctggttaat taaaggacta agttgaaaat
     7261 gaatgaatga atgaatgatt agagataaac cgataccact tttttacaaa ccgatatgag
     7321 tacaagtata atagtttctg ttactcgaca atactgagta ccgattctga tacttcttgg
     7381 atttggtttc aatgtaatta atttgaaaga aggttttatt tcttgcctgt cgcaaggatg
     7441 aacacaaaaa tctttaccag aaggatattt taagctaaga atatacgcac gttgttgaca
     7501 accctgtatc gattaccgat taaacgtgga catttaaagg ataagttatg cttgcgccat
     7561 cacaacttta cacattacat gatcaaagat catattgaat attcggttta ttcgtcagaa
     7621 gcctatagat ttgttaaaag tgtattttat aaacggatgg tttataaaga ctaagctatt
     7681 aatatatcag acattcatta atacatcaag acatcgatgt caggtaggac aacaaatcac
     7741 atccatgtaa tgaaatcttg ttgagtggta ttggtgcgtg gtttcagcat ttcatgtgca
     7801 atacgagtaa gaatatttta accaagtatc gacccaaaac ctgatactgg tatcggtgca
     7861 tcccttatta ttattattat caaaaacaca cttgttttat ttatgtgggt gccaaacaac
     7921 aaactatgaa atgcagcatg acacgcccaa aaagtgttcc tttgagaaca acttcataaa
     7981 tatgtttctt atcattatgc atgaatggtg tactgggaga gctttggtat cagaattgag
     8041 ataagagact ttggcattct aaaggacaat gacattatca cactgacagt caacaacact
     8101 ttattgctct ggaaatctta tgtcatgggg caaagctgag gttcattagg acagtacctt
     8161 atttagagat gaatgggaag gctgtagtgg gctgctatca gggaagggtg gtggtcctcg
     8221 ggggaactat ggactcacaa aagcacatct gctgtaacaa gatcccctgt gcaatctaca
     8281 aaagttacaa agagaagcat ggttggttaa aaaatagaat tgactggata tgctgaatgc
     8341 attgtttgtt gtgtgttaca tcacattaac cacagattgg tgctaaatat ttgcttatct
     8401 tataaaaacc tgtgtgttta taaagggggg tgtggatgga tgcttggcat tctttgaaaa
     8461 gatgttgggg gatggaagga ggagacctta ttgatgagta tccagtttgc agataacaag
     8521 tttttagaca ttcatactat gatgctgttt atgtctgttt tttctcattt tttctataca
     8581 caaacagcta taaaacattc acttttatat ctgatgctgt atattaaatt tcgttgtatt
     8641 tattctgttt gctctgttgg gtcatagcta gggccagaca gaatctgctg acattttttg
     8701 ctttttctgc agagaatttt gtaaacaatc tgtgatttat gcgagatttt ttcgggagta
     8761 tcgtaactaa aaacttacta tatgaaataa aaaaaaataa taccttttta acttttaata
     8821 aatatataca atgcaagtcc acctagatcc aattatttgg tcgacaaagc aagtctctca
     8881 cataatatat cttctaaaag acagaaaaca ttactttcag accatattgt aatcaaatga
     8941 acactttcat attagtcaat aatattacgg aaatgtattt aaaaactgaa taaacatata
     9001 tttacacctt tgtctttatt tctagagtgc ttttacaatg tagattgtgt caaagcagct
     9061 taacttagag gattagtata gtaaattgaa actgtgtcag tccatttttt aagagtttaa
     9121 gttcagttca gtttagttca gtgtgagtta atttttactg ctgagagtcc aaacactaaa
     9181 gagcaaatcc aaatacacaa gtcaataaat aaactcaatg atgggctaaa gatctgggga
     9241 aatctgcaga tttctgtgcg cacagattct gtgtgggcct agtcattacc tatactcaac
     9301 gtttactctg agaaacttcc atattttaga ggtaaaatgc tcagaattgt aaaaagtctt
     9361 gtcaaaatac ttggaatttg ccaaaaacaa aataaaagtc acatcacctc attgtccttg
     9421 gtatcagcaa gcacttttaa cacaatcagc tgtttttgtt tataactccc actgaaggcc
     9481 atgtgcatta ctcccaagtt acacagtaaa aagtcacatg tagtttatca tgatcactgc
     9541 tgctcaagca cagctttatc tgttattcag tgtggcaata tctgaaatgt gtgcaaaata
     9601 ttatttattg tagctgatca ccaacacttt cagtgttttt tttttctact tttaatgaga
     9661 attatgttct taggatttat ccttataaac ctctttaaaa agaaatgtta tgtctgcata
     9721 gacacgacat gaaattatga acagaaaatt taactattta agatcaagac ttgacatata
     9781 acatttctaa tgaacattaa acctgaggta ggcccacttg caaaaatgta actgctatca
     9841 ctcactttat acatcattgt gtaacttgta cacacaaaaa tagtgaagca atgtggcatg
     9901 gaatgcaggt cccattcatt caaagttatt aaagcacttt cttaaagtgc attactactg
     9961 agctaagacc agctagttgc tcatacaaca tactacaagt agtgtcatcc taatttggat
    10021 aaaaaaaatg tccattttat acatggaaaa gtactcatta gatcccccat tgtttgtatt
    10081 acggtatttc gtgaacacaa gaggtaaatg acctacagag ctgctgttgt gttagattga
    10141 aaacacaaca caggatcatg gaggcatcca tcattatgac gcattctgcc acttgtaact
    10201 ctgcacagac tttttgaaaa gtttaattga gtcttggcgt tgtcgcagaa aaatacgaaa
    10261 aattctgtgt taaaatttgc aaataaaaat tccagtttta taggaaatcg ccatggcctt
    10321 gttctttaac cagttaagct tccccttctt tcactctcaa gttgcaagaa gcaagtgtag
    10381 caatgtgcac gcgacagccg ggtgtgtgac gctggaccaa tcagagcaca gagctccgaa
    10441 agtttacctt ttatggctag agccgggtat gtgccgtcat ataaaagagc gcgcccagct
    10501 tttcagcctc actttgagct cctccacacg cagctagtgc ggaatatcat ctgcttgtaa
    10561 cccattctct taagtcgaca accccccaaa cccaaggtga gttggtttta cagtttttaa
    10621 gcgtttaatt atagtttcta tctattaatg ttaagtaaaa tgtgtaaatg gataaatatg
    10681 atttgatgaa gtctttagac agttcataac ggtctctttg tagttatcta ctaggtttga
    10741 tttataagtg gtggttcttt tgataatcta tgttacattt aataaatatt ggctgtatct
    10801 taactttttt gtagcgttag tggatgtggc aggtgagaat gtcggtaatt taacgtgacc
    10861 agtcgtaggc acgacattga acattgaata ggccggtgtg aaatgggtgt taagtcttaa
    10921 tataaagttg tgttctggct aatctctctt taacagccat aaatgtgatt tttaactaca
    10981 gttttgaggg tgctggtgag catgttgcac acttgatgga tagccggcat gggaagttct
    11041 ttgtgcaggc agtgctgcag cagggtgtga cctactttag ctagccggct aaccagctct
    11101 catctgatgt aacgtaaacc ccatcaatct aacatcggct cattcgtgct ttataagatc
    11161 gaagatcttg ttattagtaa cgggtattgg catttagttt taacttggtt aattttcttt
    11221 ttgttatgtg ttctatttaa atgtagcctg cagccccctc tgtctttaat gaagctgaag
    11281 attaacgtta acgttagtga agtgttgtat ccgctatgaa agatttgaat tttagaggtg
    11341 tggtccactt taataacggc tgcggagttc tctggctgca cttcagccgc tgtgactgtc
    11401 gaagcagagc ccatcagttt ttcaggatct cggtgatttt tgaggctaga ctatgaactg
    11461 aaccgactga gagtgaccgt ccagaaatgc tttagtgtga agtctccgct cacgaggagt
    11521 ccgtggactg actgcagatc tgtgacgcag taacacccgg cggcagacac ccgttgaaat
    11581 gcggttgtgt aattgatcgc aggcgagggt ggaagaggat gtgaaacttc atttgtgtaa
    11641 aatttaggga gtggtcctgg tgtgatgaat gtcgaaatct gttccttttt actgag
//
