LOCUS       sequence-334733-       10111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10111)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0043
FEATURES             Location/Qualifiers
     source          1..10111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1766..1976
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1788..1984
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1835..1970
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2016..2618
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2016..2035)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2512..2532
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2661..2778
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2916..4094
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3147..4085
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4042..4062
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4105..4114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5964..6552
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6017..6037)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6435..6446)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6624..6857
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6935..7056
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6972..6991)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7026..7045
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7096..7231
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7251..7270)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7252..7270)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7280..7297)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7280..7296)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7289..7311)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7438..7893
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7525..7544)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7735..7756
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7919..8023
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8024..8092,8093..8884)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8242..8261)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9055..9643
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9544..9563
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9797..9814
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9931..9952
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9967..9972,9973..9990,9991..9997)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10005..10021
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10010..10032
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10029..10045
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10029..10045
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10064..10084
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10066..10084
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag
     1741 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta
     1801 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     1861 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1921 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1981 caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc
     2041 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     2101 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     2161 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     2221 acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     2281 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     2341 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     2401 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     2461 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     2521 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     2581 gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa
     2641 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2701 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2761 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
     2821 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
     2881 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
     2941 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     3001 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     3061 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     3121 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     3181 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
     3241 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
     3301 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     3361 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     3421 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     3481 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     3541 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     3601 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     3661 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     3721 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     3781 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     3841 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     3901 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     3961 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     4021 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     4081 ttcaggtgtc gtgaggctag caccgccacc atggccatgt cccaggaatc attgaccttc
     4141 aaggacgtgt ttgtggactt caccctggag gagtggcagc aactggactc tgcccagaag
     4201 aacctctaca gggatgtcat gcttgagaac tacagccacc tggtgtccgt ggggcatcta
     4261 gttgggaagc cagatgtgat cttcaggttg ggaccaggtg acgagtcctg gatggcagat
     4321 ggagggaccc cggtacggac ctgtgcaggt gaggacaggc cagaagtctg ggaagttgac
     4381 gagcagatag atcactacaa ggaaagccaa gacaaatttc tgtggcaagc tgcattcata
     4441 ggcaaggaaa cactgaagga tgaaagtggt caagaatgta aaatatgtag aaaaatcatt
     4501 tatctcaaca cagactttgt ttctgtaaaa caaagactcc ctaaatatta ttcatgggaa
     4561 aggtgttcaa aacatcattt aaactttctt ggtcaaaata gaagctatgt aagaaagaaa
     4621 gatgatggat gtaaggcata ttggaaagta tgcctccatt ataatcttca taaagctcaa
     4681 cctgcagaga gattttttga ccctaatcaa cgagggaaag ccctccacca aaagcaagcc
     4741 cttagaaaaa gtcagagaag tcaaactggg gagaaactct acaaatgtac tgaatgtgga
     4801 aaagtgttta tccagaaagc aaacttagtt gtacatcaaa gaactcacac cggagagaaa
     4861 ccttatgaat gctgcgaatg tgcaaaagcc ttcagccaga agtcaaccct catagcacac
     4921 cagagaactc acacagggga gaagccctat gaatgcagtg aatgtggaaa aacctttatc
     4981 cagaagtcaa ctctgattaa acatcaacga actcatacag gagagaaacc atttgtatgt
     5041 gacaaatgtc caaaagcctt taagagttca tatcatctta ttagacatga aaaaacacac
     5101 attagacaag cattttataa aggtattaaa tgtactacgt ccagccttat atatcaaaga
     5161 attcacacaa gtgagaaacc tcagtgcagt gaacatggga aagcctctga tgagaagccc
     5221 agtcccacta aacattggag aactcataca aaagagaaca tttatgagtg tagtaaatgt
     5281 gggaaaagct tcagaggaaa gtcacacctc tctgttcatc agagaattca tacaggagag
     5341 aaaccctatg aatgtagtat atgtgggaag actttcagtg gaaagtcaca cctctctgtc
     5401 catcatagaa ctcatacagg agagaaacct tatgaatgca gaagatgtgg gaaagccttt
     5461 ggggagaagt caacccttat tgtacatcag agaatgcata caggagagaa accctataaa
     5521 tgcaacgaat gtgggaaagc ctttagtgag aagtcaccac tgattaaaca tcagagaatt
     5581 catacaggag agagacccta tgaatgcact gactgtaaaa aagccttcag taggaagtca
     5641 actctcatca aacatcagag aattcataca ggagaaaaac cttacaaatg tagtgaatgt
     5701 gggaaggcct tcagtgtgaa atcaactctc attgtgcatc acagaactca tacaggagag
     5761 aaaccttatg aatgcagaga ctgtgggaaa gcattcagtg ggaagtcaac tctcattaaa
     5821 catcagagaa gtcacacagg agataaaaac ctatagtaat gaactagtcc gtaacttgaa
     5881 agtatttcga tttcttggct ttatatatct tgtggaaagg acgattacgc ataaataagc
     5941 ccgcaagaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     6001 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6061 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     6121 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     6181 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     6241 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     6301 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     6361 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     6421 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     6481 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     6541 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     6601 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     6661 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6721 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6781 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6841 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
     6901 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     6961 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7021 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     7081 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7141 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7201 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     7261 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     7321 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     7381 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     7441 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     7501 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     7561 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     7621 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     7681 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     7741 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     7801 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     7861 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     7921 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7981 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     8041 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8101 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     8161 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     8221 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     8281 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     8341 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     8401 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     8461 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8521 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     8581 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8641 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8701 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8761 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8821 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8881 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8941 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     9001 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     9061 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     9121 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9181 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     9241 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9301 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9361 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9421 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9481 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9541 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9601 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9661 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     9721 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     9781 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     9841 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     9901 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     9961 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
    10021 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
    10081 aagggaacaa aagctggagc tgcaagctta a
//
