LOCUS       sequence-334736-       10282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10282)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1546
FEATURES             Location/Qualifiers
     source          1..10282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3220..3808
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3273..3293)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3691..3702)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3880..4113
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4191..4312
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4228..4247)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4282..4301
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4352..4487
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4507..4526)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4508..4526)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4536..4553)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4536..4552)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4545..4567)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4694..5149
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4781..4800)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4991..5012
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5175..5279
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5280..5348,5349..6140)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5498..5517)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6311..6899
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6800..6819
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7053..7070
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7187..7208
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7223..7228,7229..7246,7247..7253)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7261..7277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7266..7288
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7285..7301
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7285..7301
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7320..7340
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7322..7340
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7368..7594
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7595..7775
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7822..7947
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8440..8673
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8858..8902
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9133..9343
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9155..9351
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9202..9337
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9383..9985
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9383..9402)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9879..9899
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10028..10145
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgtt
     1201 tggttccagc agatacttgg gttcctctga acagcccaga gctaattcac tgggtcccag
     1261 tgacaggacc ttggttttgt gctccctagt ggaaggggaa gataaggtca acccctccga
     1321 accacatgga ctgagaatgg aggagaaatg gttgctcaaa ggaaaactga ggaatcagag
     1381 gaaccaaaac aagctcctgt ccccaaacaa gaagcagagg aaaaaccaca ctagcaagct
     1441 gcaagagttg gcactgctgc tgcccatagc cctgaagacg gggaccaaga agctcacaaa
     1501 gaaggagatt ctggtgcatg tcctgcagta cattcagtac ctccagagaa acatcgatgc
     1561 cgccaaggcc ttgttcaaat gccacatcac cactggggaa ggtggacttg cggggctggg
     1621 tcagaaacca gcctggggcc cagccaggcg gaggagacac tctaccccct ccagctcccc
     1681 aagctctcag aagtcctgtc tccagggggc gtgccagaag cctcggaaga agaagctgac
     1741 ccaggcatca gaaagccaga ctcggacccc gaagcctcgc cgctctctgg ccctgaacaa
     1801 gcctgagaag ctggtggccc catccccaga ccagaaagga agtggcacag gggggaccac
     1861 tacccctcca aggtgccctg actcctgcgg tcacccgagg cctgcatcat cctcacctcc
     1921 aggtgacaga aaaggaggac agtcccagct gacgctgttg gatctggccg aggacaccat
     1981 ccactgtgac atctcaagct gctggtgcca gggcagtgtc caggatgacg cacctttccc
     2041 tgcgctcctg gctcaggaag atgtggcgag gatccatttt ctcaacaaga cccagcccca
     2101 tcccaggcag aagctggtgt tctatgattc cagcgaggat gtggacaaag ggtccctaga
     2161 cgctgaccct tggctccctg cctggacccc agagaacagc ccccaaggga gcccactgtt
     2221 cctggggcct ccccagattg atgtctggag tggaacaggc cacccaagtg agatcctcgg
     2281 gctcagccct agccttttca gctccccagg gaaactgctg ccagacgaga tcttggagga
     2341 tgacatggag tacctgaccc aagcggcttt ctttgaagaa gtgtgcttag atctggagtc
     2401 ttcaccttca gcctacacgc aggaggctcc acaggaaaag gacacagcct ccaaggcccc
     2461 caaagaccct cctgagtccc acagcctgca ccggtcctca gtctcgctgg accactgcta
     2521 cctctcgctg agcgggaaca gcaaggcgcc atccagctcc agctccagct ccagctccag
     2581 ctccagctcg gaggacagcg actcggagcc cctgtggaag cagcgagagg atatgcaggc
     2641 caaccctgtg ggcacgccgg gctccagcga agaggacgaa gacaccacat ggacccccac
     2701 ccggctggcc tcacccctgc tggcagctga gaaaaaggcc acgaagggcc aagtagccag
     2761 ggcccctgtg aagcccaagg agaagaagaa aggcccctgc ccaccccaga tgaagaagaa
     2821 gtgtgtcaac ggcttcatca tgttctgcag gatgaaccgg aagcagtaca tccgatcctg
     2881 ccctggaacc gcttccacag ctgccaccaa ggagctggcc cagctgtggc gggtgatgac
     2941 ccagcaggag cggaggccat actgcaccaa ggctcgcagg ttcagccgcc agcacaaccg
     3001 catcgtgaag caggacggct ccagcagcga ggctgaggac tgggagacgc caaagccctt
     3061 ctaccagctg ctggccgaga aggccttgcc gctgcccccg cacctccagt agtaatgaac
     3121 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     3181 cccatgggtg taatgccaga ggtcacgcgt taagtcgaca atcaacctct ggattacaaa
     3241 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     3301 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     3361 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     3421 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     3481 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     3541 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3601 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     3661 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     3721 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     3781 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     3841 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     3901 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     3961 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     4021 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     4081 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     4141 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     4201 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     4261 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     4321 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4381 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4441 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     4501 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     4561 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     4621 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     4681 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4741 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4801 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4861 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4921 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4981 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     5041 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     5101 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     5161 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     5221 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     5281 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     5341 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     5401 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     5461 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     5521 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     5581 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5641 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     5701 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5761 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5821 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5881 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5941 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6001 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     6061 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6121 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     6181 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6241 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6301 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6361 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6421 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     6481 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6541 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6601 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6661 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6721 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6781 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6841 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6901 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6961 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     7021 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     7081 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     7141 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     7201 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     7261 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     7321 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     7381 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     7441 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     7501 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     7561 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     7621 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7681 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7741 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     7801 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     7861 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     7921 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     7981 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     8041 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     8101 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     8161 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     8221 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     8281 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     8341 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     8401 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     8461 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     8521 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     8581 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     8641 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     8701 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     8761 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     8821 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     8881 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     8941 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     9001 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     9061 aaccccgagg ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc
     9121 ccaggctccc cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata
     9181 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     9241 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     9301 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct
     9361 agaggtacca ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     9421 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     9481 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     9541 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt
     9601 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     9661 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     9721 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     9781 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     9841 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     9901 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     9961 gacccgcaag cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca
    10021 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
    10081 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
    10141 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca
    10201 ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
    10261 ggtcttgaaa ggagtgggaa tt
//
