LOCUS       sequence-334737-       10435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10435)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1554
FEATURES             Location/Qualifiers
     source          1..10435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3373..3961
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3426..3446)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3844..3855)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4033..4266
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4344..4465
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4381..4400)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4435..4454
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4505..4640
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4660..4679)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4661..4679)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4689..4706)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4689..4705)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4698..4720)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4847..5302
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4934..4953)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5144..5165
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5328..5432
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5433..5501,5502..6293)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5651..5670)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6464..7052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6953..6972
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7206..7223
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7340..7361
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7376..7381,7382..7399,7400..7406)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7414..7430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7419..7441
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7438..7454
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7438..7454
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7473..7493
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7475..7493
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7521..7747
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7748..7928
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7975..8100
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8593..8826
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9011..9055
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9286..9496
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9308..9504
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9355..9490
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9536..10138
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9536..9555)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10032..10052
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10181..10298
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgtt
     1201 caccatgaca agagccatgg aagaggctct ttttcagcac ttcatgcacc agaagctggg
     1261 gatcgcctat gccatacaca agccatttcc cttctttgaa ggcctcctag acaactccat
     1321 catcactaag agaatgtaca tggaatctct ggaagcctgt agaaatttga tccctgtatc
     1381 cagagtggtg cacaacattc tcacccaact ggagaggact tttaacctgt ctcttctggt
     1441 gacattgttc agtcaaatta acctgcgtga atatcccaat ctggtgacga tttacagaag
     1501 cttcaaacgt gttggtgctt cctatgaatg gcagagcaga gacacaccaa tcctacttga
     1561 agccccaact ggcctagcag aaggaagctc cctccatacc ccactggcgc tgcccccacc
     1621 acaaccccct caaccaagct gttcaccctg tgcgccaaga gtcagtgagc ctggaacatc
     1681 ctcccagcaa agcgatgaga tcctgagtga gtcgcccagc ccatctgacc ctgtcctgcc
     1741 tctccctgca ctcatccagg aaggaagaag cacttcagtg accaatgaca agttaacatc
     1801 caaaatgaat gcggaagaag actcagaaga gatgcccagc ctcctcacta gcactgtgca
     1861 agtggccagt gacaacctga tcccccaaat aagagataaa gaagaccctc aagagatgcc
     1921 ccactctccc ttgggctcta tgccagagat aagagataat tctccagaac caaatgaccc
     1981 agaagagccc caggaggtgt ccagcacacc ttcagacaag aaaggaaaga aaagaaaaag
     2041 atgtatctgg tcaactccaa aaaggagaca taagaaaaaa agcctcccag gagggacagc
     2101 ctcatctaga cacggaatcc aaaagaagct caaaagggtg gatcaggttc ctcaaaagaa
     2161 agatgactca acttgtaact ccacggtaga gacaagggcc caaaaggcga gaactgaatg
     2221 tgcccgaaag tcgagatcag aggagatcat tgatggcact tcagaaatga atgaaggaaa
     2281 gaggtcccag aagacgccta gtacaccacg aagggtcaca caaggggcag cctcacctgg
     2341 gcatggcatc caagagaagc tccaagtggt ggataaggtt actcaaagga aagacgactc
     2401 aacctggaac tcagaggtca tgatgagggt ccaaaaggca agaactaaat gtgcccgaaa
     2461 gtccagattg aaagaaaaga aaaaggagaa agatatctgt tcaagctcaa aaaggagatt
     2521 tcagaaaaat attcaccgaa gaggaaaacc caaaagtgac actgtggatt ttcactgttc
     2581 taagctcccc gtgacctgtg gtgaggcgaa agggatttta tataagaaga aaatgaaaca
     2641 cggatcctca gtgaagtgca ttcggaatga ggatggaact tggttaacac caaatgaatt
     2701 tgaagtcgaa ggaaaaggaa ggaacgcaaa gaactggaaa cggaatatac gttgtgaagg
     2761 aatgacccta ggagagctgc tgaagcggaa aaactcggat gaatgcgagg tgtgctgtca
     2821 agggggacaa cttctctgct gcggtacttg tccacgagtc ttccatgagg actgtcacat
     2881 cccccctgtg gaagccaaga ggatgctgtg gagttgcacc ttctgcagga tgaagaggtc
     2941 ttcaggaagc caacagtgcc atcatgtatc taagaccctg gagaggcaga tgcagcctca
     3001 ggaccagctg attcgagatt acggtgagcc ctttcaggaa gcaatgtggt tggacctggt
     3061 taaggaaagg ctgattacgg aaatgtacac ggtggcatgg tttgtgcgag acatgcgcct
     3121 gatgtttcgc aaccataaaa cattttacaa ggcttctgac tttggccagg taggacttga
     3181 cttagaggca gaatttgaaa aagatctcaa agacgtgctc ggttttcatg aagccaatga
     3241 cggcggtttc tggactcttc cttagtaatg aactagtccg taacttgaaa gtatttcgat
     3301 ttcttggctt tatatatctt gtggaaagga cgataagcat tgaattacga ctaggatacg
     3361 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3421 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     3481 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     3541 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     3601 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     3661 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     3721 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     3781 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     3841 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     3901 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     3961 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     4021 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     4081 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     4141 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     4201 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     4261 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     4321 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     4381 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     4441 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     4501 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4561 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     4621 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     4681 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     4741 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     4801 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     4861 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4921 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4981 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5041 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     5101 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5161 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5221 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     5281 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5341 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5401 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5461 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5521 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5581 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5641 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5701 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5761 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5821 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5881 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5941 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6001 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6061 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6121 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6181 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6241 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6301 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6361 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6421 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6481 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6541 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6601 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6661 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6721 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6781 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6841 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6901 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6961 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7021 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7081 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7141 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     7201 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     7261 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     7321 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     7381 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     7441 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     7501 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     7561 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     7621 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     7681 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     7741 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7801 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7861 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7921 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     7981 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     8041 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     8101 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     8161 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     8221 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     8281 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     8341 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     8401 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     8461 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     8521 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     8581 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     8641 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     8701 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     8761 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     8821 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     8881 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     8941 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     9001 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     9061 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     9121 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     9181 atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat
     9241 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     9301 caaagcatgc gtctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     9361 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     9421 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     9481 tttggaggcc taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac
     9541 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     9601 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     9661 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     9721 caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt
     9781 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     9841 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     9901 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     9961 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
    10021 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
    10081 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg
    10141 aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
    10201 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
    10261 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
    10321 gagatccact ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa agttttaaac
    10381 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaatt
//
