LOCUS       sequence-33475-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mammalian expression of the cytoplasmic domain of human ATF6with
            an HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Wang Y, Shen J, Arenzana N, Tirasophon W, Kaufman RJ, Prywes R
  TITLE     Activation of ATF6 and an ATF6 DNA binding site by the endoplasmic 
            reticulum stress response.
  JOURNAL   J Biol Chem. 2000 Sep 1. 275(35):27013-20.
  PUBMED    10856300
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Sep 1. 275(35):27013-20."
COMMENT     Sequence Label: pCGN-ATF6 (1-373)
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           87..126
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             156..182
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             162..188
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
       61 caccgggacc gatccagcct ccgggggatc ttggtggcgt gaaactcccg cacctcttcg
      121 gccagcgcct tgtagaagcg cgtatggctt ctagctatcc ttatgacgtg cctgactatg
      181 ccagcctggg aggaccttct agaatggggg agccggctgg ggttgccggc accatggagt
      241 caccttttag cccgggactc tttcacaggc tggatgaaga ttgggattct gctctctttg
      301 ctgaactcgg ttatttcaca gacactgatg agctgcaatt ggaagcagca aatgagacgt
      361 atgaaaacaa ttttgataat cttgattttg atttggattt gttaccttgg gagtcagaca
      421 tttgggacat caacaaccaa atctgtacag ttaaagatat taaggcagaa ccccagccac
      481 tttctccagc ctcctcaagt tattcagtct catctcctcg gtcagtggac tcttattctt
      541 caactcagca tgttcctgag gagttggatt tgtcttctag ttctcagatg tctccccttt
      601 ccttatatgg tgaaaactct aatagtctct cttcaccgga gccactgaag gaagataagc
      661 ctgtcactgg ttctaggaac aagactgaaa atggactgac tccaaagaaa aaaattcagg
      721 tgaattcaaa accttcaatt cagcccaagc ctttattgct tccagcagca cccaagactc
      781 aaacaaactc cagtgttcca gcaaaaacca tcattattca gacagtacca acgcttatgc
      841 cattggcaaa gcagcaacca attatcagt
//
