LOCUS       sequence-334751-       11522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the CjcCas7-11 protein only for bacterial recombinant 
            protein expression for purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11522)
  AUTHORS   Ozcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, 
            Koonin EV, Abudayyeh OO, Gootenberg JS
  TITLE     Programmable RNA targeting with the single-protein CRISPR effector 
            Cas7-11.
  JOURNAL   Nature. 2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 
            10.1038/s41586-021-03886-5.
  PUBMED    34489594
REFERENCE   2  (bases 1 to 11522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 
            10.1038/s41586-021-03886-5."
COMMENT     Sequence Label: pDF0191 CjcCas7-11 bacterial expression with
            T...
FEATURES             Location/Qualifiers
     source          1..11522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1328..2410)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     2372..2391
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(2411..2488)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     2694..2713
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2797..2816
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2797..2815
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2816..2840
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2855..2877
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2896..2913
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2923..2940
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(2953..2976,2977..3012,3013..3036)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             3037..3330
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     primer_bind     complement(8819..8837)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8833..8880
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(9055..9060,9061..9077,9078..9083))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(9110..9128)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9196..9300
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9301..9369,9370..10161)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10332..10920
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10821..10840
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11074..11091
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11106..11245
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11171..11190)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11331..11353
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(join(11347..11522,1..16))
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga
       61 taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
      121 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa
      181 tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc
      241 agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt
      301 ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg
      361 ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag
      421 taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc
      481 gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg
      541 cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat
      601 tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt
      661 cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc
      721 ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt
      781 gacgatcagc ggtccaatga tcgaagttag gctggtaaga gccgcgagcg atccttgaag
      841 ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc
      901 gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa
      961 cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc
     1021 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
     1081 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
     1141 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
     1201 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     1261 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
     1321 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1381 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
     1441 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
     1501 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
     1561 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa
     1621 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
     1681 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
     1741 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
     1801 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
     1861 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
     1921 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
     1981 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
     2041 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
     2101 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
     2161 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
     2221 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
     2281 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
     2341 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
     2401 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     2461 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
     2521 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     2581 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     2641 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     2701 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     2761 ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat
     2821 tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat
     2881 accatgggca gcagccatca tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc
     2941 catatggcta gctggagcca tccgcagttt gaaaaaggtg gtggtagcgg tggtggttca
     3001 ggtggtagtg catggtcaca ccctcagttt gagaaaatgt cggactcaga agtcaatcaa
     3061 gaagctaagc cagaggtcaa gccagaagtc aagcctgaga ctcacatcaa tttaaaggtg
     3121 tccgatggat cttcagagat cttcttcaag atcaaaaaga ccactccttt aagaaggctg
     3181 atggaagcgt tcgctaaaag acagggtaag gaaatggact ccttaagatt cttgtacgac
     3241 ggtattagaa tccaagctga tcagacccct gaagatttgg acatggagga taacgatatt
     3301 attgaggctc acagagaaca gattggtgga tccatgcaca ccatcctgcc tatccacctg
     3361 acctttctgg aaccctacag actggccgag tggcacgcca aggccgacag aaagaagaac
     3421 aagagatacc tgcggggcat gagcttcgcc cagtggcaca aggataagga cggcatcggc
     3481 aagccctaca tcaccggcac actgctgaga agcgccgtgc tgaatgccgc cgaggaactg
     3541 atcagcctga atcaaggcat gtgggccaaa gagccctgct gcaacggcaa gttcgagaca
     3601 gagaaggaca agcctgccgt gctgcggaag aggcctacca tccagtggaa aacaggcaga
     3661 cccgccatct gcgaccccga gaagcaagag aagaaggacg cctgtcctct gtgcatgctg
     3721 ctgggcagat ttgacaaggc cggcaagcgg caccgggaca acaaatacga caagcacgac
     3781 tacgacatcc acttcgacaa cctgaacctg atcaccgaca agaagttctc tcaccccgac
     3841 gatatcgcca gcgagcggat cctgaacaga gtggattaca ccaccggcaa ggcccacgac
     3901 tacttcaaag tgtgggaagt cgacgacgac cagtggtggc agtttaccgg caccatcacc
     3961 atgcacgacg actgctctaa ggccaaggga ctgctgctgg cctctctgtg cttcgtggat
     4021 aagctgtgtg gcgccctgtg ccggattgaa gtgaccggca acaacagcca ggacgagaac
     4081 aaagagtacg cccatcctga cacaggcatc atcacatccc tgaatctgaa gtaccagaac
     4141 aactccacca tccaccagga cgccgtgcca ctgtctggat ctgcccacga caatgacgag
     4201 cctccagtgc acgacaacga tagcagcctg gacaacgaca caatcaccct gctgagcatg
     4261 aaggccaaag aaatcgtggg cgccttcaga gagagcggaa agatcgagaa ggcccggaca
     4321 ctggccgatg tgatcagagc catgagactg cagaagcccg acatctggga gaagctgccc
     4381 aagggcatca acgataagca ccacctgtgg gatagagaag tgaacggaaa gaagctgcgg
     4441 aacatcctgg aagaactgtg gcggctgatg aacaagagga acgcctggcg gaccttctgc
     4501 gaggtgctgg gcaatgagct gtaccggtgc tacaaagaga aaaccggcgg catcgtgctg
     4561 agattcagaa ccctgggcga aaccgagtac taccccgagc ctgaaaagac cgagccttgc
     4621 ctgatcagcg ataacagcat ccccatcaca cctctcggcg gcgtgaaaga atggatcatc
     4681 atcggcagac tgaaggccga gacacccttc tactttggcg tgcagagcag cttcgacagc
     4741 acccaggatg acctggacct ggtgcctgac atcgtgaaca ccgacgagaa gctggaagcc
     4801 aacgagcaga ccagcttcag aatcctgatg gacaagaagg gccgctacag aatccctcgg
     4861 agcctgatta gaggcgtgct gaggcgggat ctgagaacag cctttggcgg ctctggctgc
     4921 atcgtggaac tgggcagaat gatcccctgc gactgcaaag tgtgcgccat catgcggaag
     4981 atcaccgtga tggacagcag aagcgagaac atcgagctgc ccgatatccg gtacaggatc
     5041 cggctgaatc cttacaccgc caccgtggat gagggcgctc tgttcgatat ggaaatcggc
     5101 cccgagggca tcacctttcc attcgtgttc agatacagag gcgaggacgc cctgcctcgg
     5161 gaactttgga gcgtgatcag atactggatg gacggcatgg cctggctcgg cggatctgga
     5221 tctactggca agggaagatt cgccctgatc gacatcaagg tgttcgagtg ggacctgtgc
     5281 aacgaggaag gcctgaaggc ctacatctgc agcagaggcc tgagaggcat cgagaaagag
     5341 gtgctgctgg aaaacaagac aatcgccgag atcaccaacc tgttcaagac cgaggaagtg
     5401 aagttcttcg agagctacag caagcacatc aagcagctgt gccacgagtg cattatcaac
     5461 cagatcagct tcctgtgggg cctgagaagc tactacgagt atctgggccc tctgtggacc
     5521 gaagtgaaat acgagatcaa gatcgcctct ccactgctgt cctccgacac catttctgcc
     5581 ctgctgaaca aggacaacat cgactgtatc gcctacgaga agcgcaagtg ggagaatggc
     5641 ggcatcaagt tcgtgcccac catcaagggc gagacaatca gaggaatcgt gcggatggcc
     5701 gtgggcaaga gatctggcga tctcggcatg gacgaccacg aggattgcag ctgtaccctg
     5761 tgcaccatct tcggcaacga gcacgaggcc ggaaagctga gatttgagga cctggaagtg
     5821 gtggaagaga aactgccctc cgagcagaac agcgacagca acaagatccc tttcggcccc
     5881 gttcaggacg gcgacggcaa cagagaaaaa gaatgcgtga ccgccgtgaa gtcctacaag
     5941 aagaaactga tcgaccacgt ggccatcgac cggtttcatg gcggagccga ggacaagatg
     6001 aagttcaaca cactgcctct ggccggcagc tttgagaagc ccatcatcct gaagggccga
     6061 ttctggatca agaaggacat cgtcaaggac tacaagaaaa agattgagga cgccatggtg
     6121 gacatccgcg acggactgta tcctatcggc ggcaagacag gcatcggcta cggctgggtt
     6181 accgatctga ccatcctgaa tcctcagagc ggctttcaga tccccgtgaa gaaggatatc
     6241 agccctgagc ctggcaccta cagcacatac cctagccaca gcacacctag cctgaacaaa
     6301 ggccacatct actaccctca ctactttctg gcccctgcca acaccgtgca ccgcgagcaa
     6361 gaaatgatcg gacacgagca gttccacaaa gagcagaaag gcgagctgct ggtgtccggc
     6421 aagatcgtgt gcaccctgaa aaccgtgaca cccctgatca tccccgacac cgagaacgag
     6481 gatgccttcg ggctgcagaa tacctacagc ggccacaaga actaccagtt ctttcacatc
     6541 aacgacgaga tcatggtgcc cggcagcgag atcaggggca tgatcagctc tgtgtacgag
     6601 gccatcacca atagctgctt ccgggtgtac gacgagacaa agtacatcac cagacggctg
     6661 agccccgaga aaaaggacga gagcaacgac aagaacaagt cccaggacga cgccagccag
     6721 aagatcagaa agggcctcgt gaagaaaacc gacgagggct tcagcatcat cgaggtggaa
     6781 cggtactcca tgaagacaaa aggcggcacc aagctggtgg acaaggtgta cagactgccc
     6841 ctgtacgata gcgaggccgt gattgccagc atccagttcg agcagtacgg cgagaagaat
     6901 gagaaacgga acgccaagat cagggccgcc atcaagagaa acgaagtgat cgccgaggtg
     6961 gcccggaaga acctgatttt cctgcggtcc ctgacacctg aagaactgaa gaaggtgctg
     7021 cagggcgaga tcctggtcaa gttctccctg aagtctggca agaaccccaa cgactacctg
     7081 gccgagctgc acgagaatgg cacagaaagg ggcctgatca agttcaccgg cctgaacatg
     7141 gtcaatatca agaacgtgaa cgaagaggac aaggacttca atgacacctg ggactgggag
     7201 aaactgaaca tcttccacaa cgcccacgag aagaggaata gcctgaagca gggctacccc
     7261 agacctgtgc tgaagttcat caaggaccgg gtcgagtaca caatccccaa gagatgcgag
     7321 aggatcttct gcattcctgt gaagaacacc atcgagtaca aggtgtccag caaagtctgc
     7381 aagcagtaca aggacgtgct gagcgattac gagaagaact ttggccacat caacaagatt
     7441 ttcaccacca agatccagaa gcgcgagctg accgacggcg atctggtgta cttcatccct
     7501 aacgagggcg ccgacaagac cgtgcaggct attatgccag tgccactgag cagaatcacc
     7561 gatagcagaa cactgggaga gagactgcct cacaagaacc tgctgccttg cgtgcacgaa
     7621 gtgaatgagg gcctgctgtc cggcatcctg gacagcctgg ataagaaact gctgtctatt
     7681 caccccgagg gactgtgccc tacctgcaga ctgtttggca ccacctacta caagggcaga
     7741 gtcagattcg gcttcgccaa cctcatgaac aagcccaagt ggctgaccga gagagagaac
     7801 ggctgcggcg gatatgtgac actgccactg cttgagcggc ccagactgac ttggagtgtg
     7861 cccagcgaca agtgtgacgt gccaggccgg aagttctaca tccaccacaa cggctggcaa
     7921 gaggtcctga gaaacaacga catcacccct aagaccgaga acaaccggac cgtggaacca
     7981 ctggccgccg acaacagatt caccttcgac gtgtacttcg agaacctcag agagtgggag
     8041 ctggggctgc tgtgctattg cctggaactg gaacctggca tgggccacaa gctcggaatg
     8101 ggcaagccta tgggcttcgg ctctgtgaag atcgccattg agcggctgca gaccttcaca
     8161 gtgcaccagg atggcatcaa ctggaagccc agcgagaatg agatcggcgt gtacgtgcag
     8221 aaaggacggg aaaaactggt ggaatggttc acccctagcg ctccccacaa gaatatggaa
     8281 tggaacggcg tgaagcacat taaggacctg agatccctcc tgagcatccc tggcgataag
     8341 cctaccgtga agtaccccac tctcaacaag gatgccgaag gcgccatcag cgactacacc
     8401 tacgagagac tgagcgatac caagctgctg ccccacgata agagagtgga atacctgaga
     8461 accccttgga gcccctggaa cgcctttgtg aaagaggccg agtacagccc ctccgagaag
     8521 tccgatgaga aaggcagaga gacaatccgg accaagccta agagcctgcc aagcgtgaag
     8581 tctatcggca aagtgaagtg gttcgacgaa ggcaaaggct tcgggatcct gatcatggac
     8641 gatggcaaag aggtgtccat ctccaagaac agcatcagag gcaacatcct gctcaagaag
     8701 ggacagaaag tcaccttcca catcgtgcag ggactgatcc ctaaggccga ggatatcgag
     8761 attgccaagt gagcggccgc actcgaggcc cgaaaggaag ctgagttggc tgctgccacc
     8821 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     8881 ctgaaaggag gaactatatc cggatatccc gcaagaggcc cggcagtacc ggcataacca
     8941 agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag
     9001 atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa
     9061 gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata
     9121 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     9181 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     9241 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     9301 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     9361 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     9421 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     9481 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     9541 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     9601 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     9661 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     9721 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     9781 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     9841 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     9901 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     9961 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
    10021 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
    10081 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
    10141 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
    10201 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
    10261 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    10321 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    10381 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    10441 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
    10501 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    10561 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    10621 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    10681 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    10741 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    10801 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    10861 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    10921 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    10981 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
    11041 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
    11101 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
    11161 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
    11221 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
    11281 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
    11341 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
    11401 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
    11461 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
    11521 tt
//
