LOCUS       sequence-334754-        2217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the mature DisCas7-11 DR along with a golden gate site for 
            spacer cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ozcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, 
            Koonin EV, Abudayyeh OO, Gootenberg JS
  TITLE     Programmable RNA targeting with the single-protein CRISPR effector 
            Cas7-11.
  JOURNAL   Nature. 2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 
            10.1038/s41586-021-03886-5.
  PUBMED    34489594
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 
            10.1038/s41586-021-03886-5."
COMMENT     Sequence Label: pDF0114 pU6-Eco31i-Eco31i-DisCas7-11 mature DR...
FEATURES             Location/Qualifiers
     source          1..2217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     484..503
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(722..826)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        933..1181
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     933..953
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1104..1123
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     rep_origin      complement(1319..1907)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1399..1418)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2078..2217,1..652,653..721))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gagatccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
       61 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
      121 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
      181 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
      241 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
      301 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
      361 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
      421 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
      481 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
      541 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
      601 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
      661 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
      721 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
      781 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
      841 aagtgccacc tgacgtcgct agctgtacaa aaaagcaggc tttaaaggaa ccaattcagt
      901 cgactggatc cggtaccaag gtcgggcagg aagagggcct atttcccatg attccttcat
      961 atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca
     1021 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     1081 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     1141 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgttgatgtc acggaaccga
     1201 gaccgtgagg tctcgttttt ttaagcttgg gccgctcgag gtacctctct acatatgaca
     1261 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1321 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1381 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1441 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1501 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1561 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1621 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1681 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1741 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1801 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1861 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1921 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1981 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2041 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2101 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2161 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgc
//
