LOCUS       sequence-33476-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  TITLE     Prywes Lab Plasmids
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRevTRE-FLMKL1
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..160
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(791..802)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atccgctngt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccga
       61 attcgagctc ggtacccggg gatccgccac catggactac aaagaccatg acggtgatta
      121 taaagatcat gacatcgatt acaaggatga cgatgacaag cttgcggccg cgaattcact
      181 gcccccttcc gtcattgctg tgaatgggct ggacggagga ggggccggcg aaaatgatga
      241 tgaaccagtg ctcgtgtcct tatctgcggc acccagtccc cagagtgaag ctgttgccaa
      301 tgaactgcag gagctctcct tgcagcccga gctgacccta ggcctccacc ctggcaggaa
      361 tcccaatttg cctccactta gtgagcggaa gaatgtgcta cagttgaaac tccagcagcg
      421 ccggacccgg gaagaactgg tgagccaagg gataatgccg cctttgaaaa gtccagccgc
      481 atttcatgag cagagaagga gcttggagcg ggccaggaca gaggactatc tcaaacggaa
      541 gattcgttcc cggccggaga gatcggagct ggtcaggatg cacattttgg aagagacctc
      601 ggctgagcca tccctccagg ccaagcagct gaagctgaag agagccagac tagccgatga
      661 cctcaatgag aagattgcac agaggcctgg ccccatggaa ttagttgaga agaacatcct
      721 tcctgttgag tccagcctga aggaagccat cattgtgggc caggtgaact atcccaaagt
      781 agcagacagc tcttccttcg atgaggacag cagcgatgcc ttatcccccg agcagcctgc
      841 cagccatgag tcccagggtt ctgtgccgtc acccctggag gcccgagtca gcgaaccact
      901 gctcagtgcc acctctgcat cccccaccc
//
