LOCUS       sequence-334779-        5097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  YAP/TAZ-responsive synthetic promoter driving luciferase expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Dupont S, Morsut L, Aragona M, Enzo E, Giulitti S, Cordenonsi M, 
            Zanconato F, Le Digabel J, Forcato M, Bicciato S, Elvassore N, 
            Piccolo S
  TITLE     Role of YAP/TAZ in mechanotransduction.
  JOURNAL   Nature. 2011 Jun 8;474(7350):179-83. doi: 10.1038/nature10137.
  PUBMED    21654799
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-06-8- 8"; volume: "474"; issue: "7350"; pages:
            "179-83"
COMMENT     Sequence Label: 8xGTIIC-luciferase
FEATURES             Location/Qualifiers
     source          1..5097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    238..359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(275..294)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     329..348
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(400..2052)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     1968..1986
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     regulatory      2049..2058
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2193..2209)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2194..2210)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    join(2236..2242,2243..2244,2245..2251)
                     /label=2xGTIIC
                     /bound_moiety="TEF-1 family (TEAD) proteins"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with 
                     high affinity for TEF-1 family (TEAD) proteins (Mahoney et 
                     al., 2005)"
     protein_bind    join(2268..2274,2275..2276,2277..2283)
                     /label=2xGTIIC
                     /bound_moiety="TEF-1 family (TEAD) proteins"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with 
                     high affinity for TEF-1 family (TEAD) proteins (Mahoney et 
                     al., 2005)"
     protein_bind    join(2322..2328,2329..2330,2331..2337)
                     /label=2xGTIIC
                     /bound_moiety="TEF-1 family (TEAD) proteins"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with 
                     high affinity for TEF-1 family (TEAD) proteins (Mahoney et 
                     al., 2005)"
     protein_bind    join(2354..2360,2361..2362,2363..2369)
                     /label=2xGTIIC
                     /bound_moiety="TEF-1 family (TEAD) proteins"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with 
                     high affinity for TEF-1 family (TEAD) proteins (Mahoney et 
                     al., 2005)"
     misc_feature    2426..2517
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(2458..2477)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    2531..2579
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(2710..3165)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2847..2868)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3059..3078
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3192..3296
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3297..3365,3366..4157)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3515..3534)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4328..4916
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4817..4836
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5070..5087
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg
       61 acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc
      121 atcggtcgac ggatccttat cgattttacc acatttgtag aggttttact tgctttaaaa
      181 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
      241 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
      301 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
      361 catgtctgct cgaagcggcc ggccgccccg actctagaat tacacggcga tctttccgcc
      421 cttcttggcc tttatgagga tctctctgat ttttcttgcg tcgagttttc cggtaagacc
      481 tttcggtact tcgtccacaa acacaactcc tccgcgcaac tttttcgcgg ttgttacttg
      541 actggcgacg taatccacga tctctttttc cgtcatcgtc tttccgtgct ccaaaacaac
      601 aacggcggcg ggaagttcac cggcgtcatc gtcgggaaga cctgcgacac ctgcgtcgaa
      661 gatgttgggg tgttggagca agatggattc caattcagcg ggagccacct gatagccttt
      721 gtacttaatc agagacttca ggcggtcaac gatgaagaag tgttcgtctt cgtcccagta
      781 agctatgtct ccagaatgta gccatccatc cttgtcaatc aaggcgttgg tcgcttccgg
      841 attgtttaca taaccggaca taatcatagg acctctcaca cacagttcgc ctctttgatt
      901 aacgcccagc gttttcccgg tatccagatc cacaaccttc gcttcaaaaa atggaacaac
      961 tttaccgacc gcgcccggtt tatcatcccc ctcgggtgta atcagaatag ctgatgtagt
     1021 ctcagtgagc ccatatcctt gcctgatacc tggcagatgg aacctcttgg caaccgcttc
     1081 cccgacttcc ttagagaggg gagcgccacc agaagcaatt tcgtgtaaat tagataaatc
     1141 gtatttgtca atcagagtgc ttttggcgaa gaaggagaat agggttggca ccagcagcgc
     1201 actttgaatc ttgtaatcct gaaggctcct cagaaacagc tcttcttcaa atctatacat
     1261 taagacgact cgaaatccac atatcaaata tccgagtgta gtaaacattc caaaaccgtg
     1321 atggaatgga acaacactta aaatcgcagt atccggaatg atttgattgc caaaaatagg
     1381 atctctggca tgcgagaatc tcacgcaggc agttctatga ggcagagcga cacctttagg
     1441 cagaccagta gatccagagg agttcatgat cagtgcaatt gtcttgtccc tatcgaagga
     1501 ctctggcaca aaatcgtatt cattaaaacc gggaggtaga tgagatgtga cgaacgtgta
     1561 catcgactga aatccctggt aatccgtttt agaatccatg ataataattt tttggatgat
     1621 tgggagcttt ttttgcacgt tcaaaatttt ttgcaacccc tttttggaaa cgaacaccac
     1681 ggtaggctgc gaaatgccca tactgttgag caattcacgt tcattataaa tgtcgttcgc
     1741 gggcgcaact gcaactccga taaataacgc gcccaacacc ggcataaaga attgaagaga
     1801 gttttcactg catacgacga ttctgtgatt tgtattcagc ccatatcgtt tcatagcttc
     1861 tgccaaccga acggacattt cgaagtactc agcgtaagtg atgtccacct cgatatgtgc
     1921 atctgtaaaa gcaattgttc caggaaccag ggcgtatctc ttcatagcct tatgcagttg
     1981 ctctccagcg gttccatctt ccagcggata gaatggcgcc gggcctttct ttatgttttt
     2041 ggcgtcttcc atggtggctt taccaacagt accggaatgc caagctcttg ggggatcgtc
     2101 ccacggagcg gtgggtgccg gcggctgtct gggaagggct ccttgggggg cagaggcttt
     2161 aaggtccccc cggcgcccac ccccaagctt atcgataccg tcgacctcga cctgcagatc
     2221 tctagaagct tgcagtggaa tgtgtggaat gtgtctcaag cttgcagtgg aatgtgtgga
     2281 atgtgtctca acttggcact ggccgggcta gaagcttgca gtggaatgtg tggaatgtgt
     2341 ctcaagcttg cagtggaatg tgtggaatgt gtctcaactt ggcactggcc gggctagcac
     2401 gcgtaagagc tcggtaccta tcgatagaga aatgttctgg cacctgcact tgcactgggg
     2461 acagcctatt ttgctagttt gttttgtttc gttttgtttt gatggagagc gtatgttagt
     2521 actatcgatt cacacaaaaa accaacacac agatgtaatg aaaataaaga tattttattg
     2581 cggccgctcc aagtacctcc cgtaccttaa tattacttac ttatcatggt agcttgggct
     2641 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     2701 gcgaatggca aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     2761 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     2821 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     2881 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     2941 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     3001 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     3061 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     3121 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     3181 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     3241 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     3301 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     3361 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     3421 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     3481 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     3541 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     3601 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     3661 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     3721 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     3781 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     3841 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     3901 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     3961 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     4021 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     4081 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     4141 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     4201 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4261 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4321 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4381 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4441 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     4501 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4561 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4621 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4681 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4741 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4801 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4861 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4921 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4981 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     5041 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag
//
