LOCUS       sequence-33479-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Cen B, Selvaraj A, Burgess RC, Hitzler JK, Ma Z, Morris SW, Prywes R
  TITLE     Megakaryoblastic leukemia 1, a potent transcriptional coactivator 
            for serum response factor (SRF), is required for serum induction of 
            SRF target genes.
  JOURNAL   Mol Cell Biol. 2003 Sep . 23(18):6597-608.
  PUBMED    12944485
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2003 Sep . 23(18):6597-608."
COMMENT     Sequence Label: p3XFLAG-MKL1-~100
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..106
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(161..172)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgtggtttgc gactgtttgt gaccgtcaga attaaccatg gactacaaag accatgacgg
       61 tgattataaa gatcatgaca tcgattacaa ggatgacgat gacaagcttg cggccgcgaa
      121 ttcggtgggc caggtgaact atcccaaagt agcagacagc tcttccttcg atgaggacag
      181 cagcgatgcc ttatcccccg agcagcctgc cagccatgag tcccagggtt ctgtgccgtc
      241 acccctggag gcccgagtca gcgaaccact gctcagtgcc acctctgcat cccccaccca
      301 ggttgtgtct caacttccga tgggccggga ttccagagaa atgcttttcc tggcagagca
      361 gcctcctctg cctcccccac ctctgctgcc tcccagcctc accaatggaa ccactatccc
      421 cactgccaag tccaccccca cactcattaa gcaaagccaa cccaagtctg ccagtgagaa
      481 gtcacagcgc agcaagaagg ccaaggagct gaagccaaag gtgaagaagc tcaagtacca
      541 ccagtacatc cccccggacc agaagcagga caggggggca ccccccatgg actcatccta
      601 cgccaagatc ctgcagcagc agcagctctt cctccagctg cagatcctca accagcagca
      661 gcagcagcac cacaactacc aggccatcct gcctgccccg ccaaagtcag caggcgaggc
      721 cctgggaagc agcgggaccc ccccagtacg cagcctctcc actaccaata gcagctccag
      781 ctcgggcgcc cctgggccct gtgggctggc acgtcagaac agcacctcac tgactggcaa
      841 gccgggagcc ctgccggcca acctggacga catgaaggtg gcagagctga agcaggagct
      901 gaagttgcga tcactgcctg tctcgggcac caaaactgac tat
//
