LOCUS       sequence-334828-        9626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of TRF1-mcherrry and sgRNA targeting telomeres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Wang H, Xu X, Nguyen CM, Liu Y, Gao Y, Lin X, Daley T, Kipniss NH, 
            La Russa M, Qi LS
  TITLE     CRISPR-Mediated Programmable 3D Genome Positioning and Nuclear 
            Organization.
  JOURNAL   Cell. 2018 Nov 15;175(5):1405-1417.e14. doi: 
            10.1016/j.cell.2018.09.013. Epub 2018 Oct 11.
  PUBMED    30318144
REFERENCE   2  (bases 1 to 9626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.013"; journalName: "Cell"; date: 
            "2018-11-15- 15"; volume: "175"; issue: "5"; pages: "1405-1417.e14"
COMMENT     Sequence Label: pHR-U6-sgTel-CMV-puro-P2A-mCherry-TRF1
FEATURES             Location/Qualifiers
     source          1..9626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        40..243
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             277..873
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(277..296)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     773..793
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             883..936
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      943..952
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             949..1653
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELY"
     primer_bind     complement(1094..1112)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1362..1381
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2996..3029)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3085..3673
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3138..3158)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3556..3567)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3676..3692)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3677..3693)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4202..4382
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(4444..5032)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4524..4543)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5203..5994,5995..6063))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5826..5845
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6064..6168)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6243..6262)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6434..6813
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6815..7013
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7031..7211
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7258..7383
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7880..8113
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8298..8342
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8640..8757
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(9010..9043)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     9047..9067
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     enhancer        join(9362..9626,1..39)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
       61 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      121 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      181 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
      241 gctggtttag tgaaccgtca gatccgctag cccaccatga ccgagtacaa gcccacggtg
      301 cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc
      361 gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag
      421 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
      481 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
      541 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
      601 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
      661 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
      721 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
      781 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
      841 cgcacctggt gcatgacccg caagcccggt gccggatcgg gagagggcag aggaagtctg
      901 ctaacatgcg gtgacgtcga ggagaatcct ggcccaccgg tcgccaccat ggtgagcaag
      961 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     1021 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1081 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     1141 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     1201 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     1261 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     1321 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     1381 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     1441 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     1501 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     1561 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     1621 cgccactcca ccggcggcat ggacgagctg tacagatccg cggaggatgt ttcctcagcg
     1681 gccccgagcc cgcggggctg tgcggatggt agggatgccg accctactga ggagcagatg
     1741 gcagaaacag agagaaacga cgaggagcag ttcgaatgcc aggaactgct cgagtgccag
     1801 gtgcaggtgg gggcccccga ggaggaggag gaggaggagg aggacgcggg cctggtggcc
     1861 gaggccgagg ccgtggctgc cggctggatg ctcgatttcc tctgcctctc tctttgccga
     1921 gctttccgcg acggccgctc cgaggacttc cgcaggaccc gcaacagcgc agaggctatt
     1981 attcatggac tatccagtct aacagcttgc cagttgagaa cgatatacat atgtcagttt
     2041 ttgacaagaa ttgcagcagg aaaaaccctt gatgcacagt ttgaaaatga tgaacgaatt
     2101 acacccttgg aatcagccct gatgatttgg ggttcaattg aaaaggaaca tgacaaactt
     2161 catgaagaaa tacagaattt aattaaaatt caggctatag ctgtttgtat ggaaaatggc
     2221 aactttaaag aagcagaaga agtctttgaa agaatatttg gtgatccaaa ttctcatatg
     2281 cctttcaaaa gcaaattgct tatgataatc tctcagaaag atacatttca ttcctttttt
     2341 caacacttca gctacaacca catgatggag aaaattaaga gttatgtgaa ttatgtgcta
     2401 agtgaaaaat catcaacctt tctaatgaag gcagcggcaa aagtagtaga aagcaaaagg
     2461 acaagaacaa taacttctca agataaacct agtggtaatg atgttgaaat ggaaactgaa
     2521 gctaatttgg atacaagaaa aagtgttagt gacaaacagt ctgcggtaac tgaatcctca
     2581 gagggtacag tatccttatt gaggtctcac aagaatcttt tcttatctaa gttgcaacat
     2641 ggaacccagc aacaagacct taataagaaa gaaagaagag taggaactcc tcaaagtaca
     2701 aaaaagaaaa aagaaagcag aagagccact gaaagcagaa tacctgtttc aaagagtcag
     2761 ccggtaactc ctgaaaaaca tcgagctaga aaaagacagg catggctttg ggaagaagac
     2821 aagaatttga gatctggcgt gaggaaatat ggagagggaa actggtctaa aatactgttg
     2881 cattataaat tcaacaaccg gacaagtgtc atgttaaaag acagatggag gaccatgaag
     2941 aaactaaaac tgatttcctc agacagcgaa gactgagaat tcgtcgaggg acctaataac
     3001 ttcgtatagc atacattata cgaagttata catgtttaag ggttccggtt ccactaggta
     3061 caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     3121 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3181 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3241 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3301 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3361 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3421 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     3481 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3541 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3601 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3661 gccgcctccc cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac atggagcaat
     3721 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     3781 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     3841 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     3901 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     3961 ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta
     4021 caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg
     4081 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg
     4141 gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc cggactgtac
     4201 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     4261 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     4321 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     4381 cagcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4441 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4501 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4561 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4621 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4681 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4741 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4801 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4861 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     4921 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4981 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5041 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5101 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5161 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5221 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5281 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5341 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5401 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5461 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5521 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5581 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5641 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5701 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5761 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5821 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5881 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5941 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6001 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6061 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     6121 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6181 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
     6241 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     6301 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     6361 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     6421 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     6481 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     6541 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     6601 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     6661 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     6721 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     6781 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     6841 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     6901 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     6961 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt
     7021 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7081 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7141 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7201 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     7261 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     7321 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     7381 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     7441 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     7501 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     7561 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     7621 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     7681 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca
     7741 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     7801 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     7861 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     7921 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     7981 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     8041 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     8101 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     8161 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     8221 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     8281 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     8341 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     8401 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     8461 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     8521 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     8581 tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat
     8641 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     8701 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     8761 gtttattaca gggacagcag agatccagtt tggttagtac cgggcccgct ctagagatcc
     8821 gacgcgccat ctctaggccc gcgccggccc cctcgcacgg acttgtggga gaagctcggc
     8881 tactcccctg ccccggttaa tttgcatata atatttccta gtaactatag aggcttaatg
     8941 tgcgataaaa gacagataat ctgttctttt taatactagc tacattttac atgataggct
     9001 tggatttcta taacttcgta tagcatacat tatacgaagt tataaacagc acaaaaggaa
     9061 actcacccta actgtaaagt aattgtgtgt tttgagacta taagtatccc ttggagaacc
     9121 accttgttgg ttagggttag ggttagggtt agtttaagag ctatgctgga aacagcatag
     9181 caagtttaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     9241 ttttctcgag tactaggatc cattaggcgg ccgcgtggat aaccgtatta ccgccatgca
     9301 ttagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     9361 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     9421 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     9481 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     9541 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     9601 acatgacctt atgggacttt cctact
//
