LOCUS       sequence-334831-       12016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of PYL1-SfGFP-PML, lentivirus vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12016)
  AUTHORS   Wang H, Xu X, Nguyen CM, Liu Y, Gao Y, Lin X, Daley T, Kipniss NH, 
            La Russa M, Qi LS
  TITLE     CRISPR-Mediated Programmable 3D Genome Positioning and Nuclear 
            Organization.
  JOURNAL   Cell. 2018 Nov 15;175(5):1405-1417.e14. doi: 
            10.1016/j.cell.2018.09.013. Epub 2018 Oct 11.
  PUBMED    30318144
REFERENCE   2  (bases 1 to 12016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.013"; journalName: "Cell"; date: 
            "2018-11-15- 15"; volume: "175"; issue: "5"; pages: "1405-1417.e14"
COMMENT     Sequence Label: pHR-TRE3G-PYL1-sfGFP-PML
FEATURES             Location/Qualifiers
     source          1..12016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2050..2167
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2229..2578
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    2237..2255
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2273..2291
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2309..2327
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2345..2363
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2381..2399
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2417..2435
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2453..2471
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2517..2541
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3188..3898
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             3188..3898
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(3215..3243)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3838..3858
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    5880..6468
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5933..5953)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6351..6362)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6471..6487)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6472..6488)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6578..6811
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6836..6854)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6837..6854)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7144..7163)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7377..7396)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7496..7518
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7556..7574)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7642..7746
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7747..7815,7816..8607)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7965..7984)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8778..9366
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9267..9286
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9520..9537
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9607..9627)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9612..9941
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9792..9927
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11075..11140
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11270..11290
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11715..11849
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11752..11771)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11806..11825
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
     2041 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2161 aaattttcgg gtttattaca gggacagcag agatccagtt tatcgatgag gccctttcgt
     2221 cttcactcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
     2281 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
     2341 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
     2401 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
     2461 agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg gcgcctataa aagcagagct
     2521 cgtttagtga accgtcagat cgcctggagc aattccacaa cacttttgtc ttatacttac
     2581 gcgtctgcag gatatcaagc ttgactgagt catgggtggg ggcgcgccaa ctcaagacga
     2641 attcacccaa ctctcccaat caatcgccga gttccacacg taccaactcg gtaacggccg
     2701 ttgctcatct ctcctagctc agcgaatcca cgcgccgccg gaaacagtat ggtccgtggt
     2761 gagacgtttc gataggccac agatttacaa acacttcatc aaaagctgta acgtgagtga
     2821 agatttcgag atgcgagtgg gatgcacgcg cgacgtgaac gtgataagtg gattaccggc
     2881 gaatacgtct cgagagagat tagatctgtt ggacgatgat cggagagtga ctgggtttag
     2941 tataaccggt ggtgaacata ggctgaggaa ttataaatcg gttacgacgg ttcatagatt
     3001 tgagaaagaa gaagaagaag aaaggatctg gaccgttgtt ttggaatctt atgttgttga
     3061 tgtaccggaa ggtaattcgg aggaagatac gagattgttt gctgatacgg ttattagatt
     3121 gaatcttcag aaacttgctt cgatcactga agctatgaac gtggatccag gtggaggtgg
     3181 aagcggtagc aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
     3241 tggtgatgtt aatgggcaca aattttctgt ccgtggagag ggtgaaggtg atgctacaaa
     3301 cggaaaactc acccttaaat ttatttgcac tactggaaaa ctacctgttc cgtggccaac
     3361 acttgtcact actctgacct atggtgttca atgcttttcc cgttatccgg atcacatgaa
     3421 acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaac gcactatatc
     3481 tttcaaagat gacgggacct acaagacgcg tgctgaagtc aagtttgaag gtgataccct
     3541 tgttaatcgt atcgagttaa agggtattga ttttaaagaa gatggaaaca ttcttggaca
     3601 caaactcgag tacaacttta actcacacaa tgtatacatc acggcagaca aacaaaagaa
     3661 tggaatcaaa gctaacttca aaattcgcca caacgttgaa gatggttccg ttcaactagc
     3721 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     3781 ttacctgtcg acacaatctg tcctttcgaa agatcccaac gaaaagcgtg accacatggt
     3841 ccttcttgag tttgtaactg ctgctgggat tacacatggc atggatgagc tctacaaagg
     3901 tggaggtcgg accgaagagc ccgagtctgc cggcatggag cctgcacccg cccgatctcc
     3961 gaggccccag caggaccccg cccggcccca ggagcccacc atgcctcccc ccgagacccc
     4021 ctctgaaggc cgccagccca gccccagccc cagccctaca gagcgagccc ccgcttcgga
     4081 ggaggagttc cagtttctgc gctgccagca atgccaggcg gaagccaagt gcccgaagct
     4141 gctgccttgt ctgcacacgc tgtgctcagg atgcctggag gcgtcgggca tgcagtgccc
     4201 catctgccag gcgccctggc ccctaggtgc agacacaccc gccctggata acgtcttttt
     4261 cgagagtctg cagcggcgcc tgtcggtgta ccggcagatt gtggatgcgc aggctgtgtg
     4321 cacccgctgc aaagagtcgg ccgacttctg gtgctttgag tgcgagcagc tcctctgcgc
     4381 caagtgcttc gaggcacacc agtggttcct caagcacgag gcccggcccc tagcagagct
     4441 gcgcaaccag tcggtgcgtg agttcctgga cggcacccgc aagaccaaca acatcttctg
     4501 ctccaacccc aaccaccgca cccctacgct gaccagcatc tactgccgag gatgttccaa
     4561 gccgctgtgc tgctcgtgcg cgctccttga cagcagccac agtgagctca agtgcgacat
     4621 cagcgcagag atccagcagc gacaggagga gctggacgcc atgacgcagg cgctgcagga
     4681 gcaggatagt gcctttggcg cggttcacgc gcagatgcac gcggccgtcg gccagctggg
     4741 ccgcgcgcgt gccgagaccg aggagctgat ccgcgagcgc gtgcgccagg tggtagctca
     4801 cgtgcgggct caggagcgcg agctgctgga ggctgtggac gcgcggtacc agcgcgacta
     4861 cgaggagatg gccagtcggc tgggccgcct ggatgctgtg ctgcagcgca tccgcacggg
     4921 cagcgcgctg gtgcagagga tgaagtgcta cgcctcggac caggaggtgc tggacatgca
     4981 cggtttcctg cgccaggcgc tctgccgcct gcgccaggag gagccccaga gcctgcaagc
     5041 tgccgtgcgc accgatggct tcgacgagtt caaggtgcgc ctgcaggacc tcagctcttg
     5101 catcacccag gggaaagatg cagctgtatc caagaaagcc agcccagagg ctgccagcac
     5161 tcccagggac cctattgacg ttgacctgcc cgaggaggca gagagagtga aggcccaggt
     5221 tcaggccctg gggctggctg aagcccagcc tatggctgtg gtacagtcag tgcccggggc
     5281 acaccccgtg ccagtgtacg ccttctccat caaaggccct tcctatggag aggatgtctc
     5341 caatacaacg acagcccaga agaggaagtg cagccagacc cagtgcccca ggaaggtcat
     5401 caagatggag tctgaggagg ggaaggaggc aaggttggct cggagctccc cggagcagcc
     5461 caggcccagc acctccaagg cagtctcacc accccacctg gatggaccgc ctagccccag
     5521 gagccccgtc ataggaagtg aggtcttcct gcccaacagc aaccacgtgg ccagtggcgc
     5581 cggggaggca gaggaacgcg ttgtggtgat cagcagctcg gaagactcag atgccgaaaa
     5641 ctcgtcctcc cgagagctgg atgacagcag cagtgagtcc agtgacctcc agctggaagg
     5701 ccccagcacc ctcagggtcc tggacgagaa ccttgctgac ccccaagcag aagacagacc
     5761 tctggttttc tttgacctca agattgacaa tgaaagtggg ttctcctggg gctaccccca
     5821 cccctttcta atttagtagt aacctgcagg catgcaagct tgatatcaag cttatcgata
     5881 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5941 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     6001 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     6061 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     6121 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     6181 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     6241 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     6301 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     6361 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     6421 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     6481 gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta caaggcagct
     6541 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     6601 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     6661 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6721 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6781 cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat
     6841 agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt
     6901 ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat
     6961 catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg
     7021 gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc
     7081 agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     7141 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     7201 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
     7261 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
     7321 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca
     7381 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     7441 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     7501 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     7561 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     7621 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7681 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7741 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7801 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7861 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     7921 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     7981 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     8041 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     8101 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     8161 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     8221 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     8281 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     8341 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     8401 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     8461 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     8521 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     8581 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     8641 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     8701 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8761 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8821 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8881 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     8941 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     9001 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     9061 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     9121 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     9181 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     9241 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9301 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9361 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9421 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     9481 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     9541 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gtcggccgat tcattaatgc
     9601 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     9661 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     9721 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     9781 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     9841 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     9901 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag
     9961 gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa
    10021 aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg
    10081 caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac
    10141 atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca
    10201 agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg
    10261 taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt
    10321 ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg
    10381 gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc
    10441 gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc
    10501 tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata
    10561 tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc
    10621 cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag
    10681 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
    10741 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
    10801 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
    10861 tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat
    10921 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg
    10981 atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
    11041 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
    11101 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
    11161 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
    11221 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
    11281 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
    11341 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
    11401 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
    11461 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
    11521 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
    11581 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
    11641 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
    11701 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    11761 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    11821 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
    11881 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
    11941 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
    12001 ctacaaactt agtagt
//
