LOCUS       sequence-334833-       14871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of AB1-fused-dcas9 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14871)
  AUTHORS   Wang H, Xu X, Nguyen CM, Liu Y, Gao Y, Lin X, Daley T, Kipniss NH, 
            La Russa M, Qi LS
  TITLE     CRISPR-Mediated Programmable 3D Genome Positioning and Nuclear 
            Organization.
  JOURNAL   Cell. 2018 Nov 15;175(5):1405-1417.e14. doi: 
            10.1016/j.cell.2018.09.013. Epub 2018 Oct 11.
  PUBMED    30318144
REFERENCE   2  (bases 1 to 14871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.013"; journalName: "Cell"; date: 
            "2018-11-15- 15"; volume: "175"; issue: "5"; pages: "1405-1417.e14"
COMMENT     Sequence Label: pHR-PGK-ABI-tagBFP-dCas9
FEATURES             Location/Qualifiers
     source          1..14871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(84..104)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        89..418
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      269..404
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1552..1617
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1747..1767
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2192..2326
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2229..2248)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2283..2302
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2494..3127
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3174..3299
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3796..4029
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4214..4258
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4543..4660
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4813..5220
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             6184..6882
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     regulatory      6898..6907
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6904..6930,6931..6933,6934..9420,9421..9423,
                     9424..11007)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6904..11007
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11011..11037
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             11017..11043
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             11056..11076
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11083..11103
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    11228..11816
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11281..11301)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11699..11710)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11819..11835)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11820..11836)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11926..12159
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(12184..12202)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(12185..12202)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(12492..12511)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(12725..12744)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12844..12866
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12904..12922)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12990..13094
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13095..13163,13164..13955)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13313..13332)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14126..14714
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14615..14634
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(14868..14871,1..14)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
       61 ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
      121 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
      181 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      241 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      301 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
      361 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
      421 gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag
      481 ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag
      541 atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac
      601 aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc
      661 atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg
      721 taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
      781 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
      841 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
      901 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
      961 accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat
     1021 tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
     1081 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc
     1141 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
     1201 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
     1261 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
     1321 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
     1381 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
     1441 accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc
     1501 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
     1561 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
     1621 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
     1681 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
     1741 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
     1801 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
     1861 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
     1921 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
     1981 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
     2041 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca
     2101 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     2161 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     2221 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     2281 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
     2341 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
     2401 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
     2461 tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa
     2521 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
     2581 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
     2641 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
     2701 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
     2761 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
     2821 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
     2881 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
     2941 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     3001 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     3061 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     3121 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     3181 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     3241 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     3301 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     3361 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     3421 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     3481 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     3541 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     3601 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc
     3661 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     3721 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     3781 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     3841 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     3901 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     3961 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     4021 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     4081 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     4141 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     4201 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     4261 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     4321 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     4381 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     4441 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     4501 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
     4561 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     4621 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     4681 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
     4741 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
     4801 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
     4861 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
     4921 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
     4981 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
     5041 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
     5101 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
     5161 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
     5221 ggggatctgg agctctcgag aattctcacg cgtgccacca tgacgcgtgt gcctttgtat
     5281 ggttttactt cgatttgtgg aagaagacct gagatggaag ctgctgtttc gactatacca
     5341 agattccttc aatcttcctc tggttcgatg ttagatggtc ggtttgatcc tcaatccgcc
     5401 gctcatttct tcggtgttta cgacggccat ggcggttctc aggtagcgaa ctattgtaga
     5461 gagaggatgc atttggcttt ggcggaggag atagctaagg agaaaccgat gctctgcgat
     5521 ggtgatacgt ggctggagaa gtggaagaaa gctcttttca actcgttcct gagagttgac
     5581 tcggagattg agtcagttgc gccggagacg gttgggtcaa cgtcggtggt tgccgttgtt
     5641 ttcccgtctc acatcttcgt cgctaactgc ggtgactcta gagccgttct ttgccgcggc
     5701 aaaactgcac ttccattatc cgttgaccat aaaccggata gagaagatga agctgcgagg
     5761 attgaagccg caggagggaa agtgattcag tggaatggag ctcgtgtttt cggtgttctc
     5821 gccatgtcga gatccattgg cgatagatac ttgaaaccat ccatcattcc tgatccggaa
     5881 gtgacggctg tgaagagagt aaaagaagat gattgtctga ttttggcgag tgacggggtt
     5941 tgggatgtaa tgacggatga agaagcgtgt gagatggcaa ggaagcggat tctcttgtgg
     6001 cacaagaaaa acgcggtggc tggggatgca tcgttgctcg cggatgagcg gagaaaggaa
     6061 gggaaagatc ctgcggcgat gtccgcggct gagtatttgt caaagctggc gatacagaga
     6121 ggaagcaaag acaacataag tgtggtggtg gttgatttga agtcaagcgc tgcaggtgct
     6181 accatgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     6241 gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     6301 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     6361 actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc
     6421 ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
     6481 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     6541 aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc
     6601 tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac
     6661 atggccctga agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga
     6721 tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg
     6781 gaaagaatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc
     6841 agatactgcg acctccctag caaactgggg cacaagctta attcaagcgc tgcaggtgcc
     6901 accatggaca agaagtacag catcggcctg gccatcggca ccaactctgt gggctgggcc
     6961 gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac
     7021 cggcacagca tcaagaagaa cctgatcggc gccctgctgt tcgacagcgg agaaacagcc
     7081 gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc
     7141 tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac
     7201 agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc
     7261 ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga
     7321 aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc
     7381 cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc
     7441 gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac
     7501 cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc
     7561 agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcggc
     7621 aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc
     7681 gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg
     7741 gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc
     7801 atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc
     7861 tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg
     7921 cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac
     7981 gccggctaca tcgatggcgg agccagccag gaagagttct acaagttcat caagcccatc
     8041 ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg
     8101 cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg
     8161 cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag
     8221 atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac
     8281 agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag
     8341 gaagtggtgg acaagggcgc cagcgcccag agcttcatcg agcggatgac caacttcgat
     8401 aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc
     8461 gtgtacaacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc
     8521 ctgagcggcg agcagaaaaa agccatcgtg gacctgctgt tcaagaccaa ccggaaagtg
     8581 accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa
     8641 atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa
     8701 attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc
     8761 gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat
     8821 gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg
     8881 ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc
     8941 ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac
     9001 gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc
     9061 ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag
     9121 acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc
     9181 gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag
     9241 agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac
     9301 cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg
     9361 cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac
     9421 gctatcgtgc ctcagagctt tctgaaggac gactccatcg ataacaaagt gctgactcgg
     9481 agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg
     9541 aagaactact ggcgccagct gctgaatgcc aagctgatta cccagaggaa gttcgacaat
     9601 ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga
     9661 cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg
     9721 aacactaagt acgacgagaa cgacaaactg atccgggaag tgaaagtgat caccctgaag
     9781 tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac
     9841 aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa
     9901 aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg
     9961 aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac
    10021 agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag
    10081 cggcctctga tcgagacaaa cggcgaaaca ggcgagatcg tgtgggataa gggccgggac
    10141 tttgccaccg tgcggaaagt gctgtctatg ccccaagtga atatcgtgaa aaagaccgag
    10201 gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag cgacaagctg
    10261 atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg
    10321 gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt
    10381 gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc
    10441 gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct
    10501 aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa
    10561 ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc
    10621 agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg
    10681 gaacagcaca aacactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga
    10741 gtgatcctgg ccgacgctaa tctggacaag gtgctgagcg cctacaacaa gcacagagac
    10801 aagcctatca gagagcaggc cgagaatatc atccacctgt ttaccctgac caatctggga
    10861 gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaagaggta caccagcacc
    10921 aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg
    10981 atcgacctgt ctcagctggg aggcgacgcc tatccctatg acgtgcccga ttatgccagc
    11041 ctgggcagcg gctcccccaa gaaaaaacgc aaggtggaag atcctaagaa aaagcggaaa
    11101 gtggacggca ttggtagtgg gagcaacgga gcggccgcaa ctcccacctg caacatgcgt
    11161 gactgactga ggccgcgact ctagagtcga cctgcaggca tgcaagcttg atatcaagct
    11221 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
    11281 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
    11341 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
    11401 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
    11461 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
    11521 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
    11581 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
    11641 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
    11701 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
    11761 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
    11821 ataccgtcga cctcgaggga attaattcga gctcggtacc tttaagacca atgacttaca
    11881 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
    11941 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
    12001 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
    12061 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
    12121 ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg
    12181 tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta
    12241 gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca
    12301 gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg
    12361 agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat
    12421 cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
    12481 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
    12541 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
    12601 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
    12661 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc
    12721 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
    12781 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
    12841 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
    12901 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
    12961 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    13021 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    13081 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    13141 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    13201 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    13261 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    13321 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
    13381 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    13441 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    13501 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    13561 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    13621 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    13681 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    13741 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    13801 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    13861 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    13921 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    13981 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    14041 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    14101 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    14161 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    14221 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    14281 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    14341 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    14401 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    14461 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    14521 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    14581 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    14641 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    14701 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    14761 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    14821 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc g
//
