LOCUS       sequence-334837-        9040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  alphaA-crystallin promoter (cry) drives mCherry expression in the 
            lens. The 600bp unc-45b muscle-specific promoter drives expression 
            of GFP-tagged zebrafish cct3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9040)
  AUTHORS   Berger J, Berger S, Li M, Jacoby AS, Arner A, Bavi N, Stewart AG, 
            Currie PD
  TITLE     In Vivo Function of the Chaperonin TRiC in alpha-Actin Folding 
            during Sarcomere Assembly.
  JOURNAL   Cell Rep. 2018 Jan 9;22(2):313-322. doi: 
            10.1016/j.celrep.2017.12.069.
  PUBMED    29320728
REFERENCE   2  (bases 1 to 9040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.12"; journalName: "Cell Rep"; date: 
            "2018-01-9- 9"; volume: "22"; issue: "2"; pages: "313-322"
COMMENT     Sequence Label: pcry:mCherry,-600unc:cct3GFP
FEATURES             Location/Qualifiers
     source          1..9040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    1056..1177
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1300..2010)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEDDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGHHSTGGMDELYK"
     primer_bind     complement(1380..1403)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1578..1597)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1847..1865
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2007..2016
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2657..2673
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2693..2713
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3316..3340
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4460..4462,4463..4465,4466..5176)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4460..4462,4463..4465,4466..5176)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4505..4526)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4766..4785)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5113..5134
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5714..5738)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5740..5874
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(5937..5957)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(5965..5981)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5997..6131
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(6570..6757,6758..6769)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6803..6819)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6803..6819)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6856..6876)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6856..6874)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6895..6911)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6908..6930)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6919..6935
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6943..6949,6950..6967,6968..6973))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6988..7009
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7126..7143)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7297..7885)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7377..7396)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8056..8847,8848..8916))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8679..8698
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8917..9021)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggcccgatt ggccttaaga tacattgatg agtttggaca
     1081 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     1141 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     1201 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     1261 atgtggtatg gctgattatg atctagagtc gcggccgctt tacttgtaca gctcgtccat
     1321 gccgccggtg gagtggtggc cctcggcgcg ctcgtactgt tccacgatgg tgtagtcctc
     1381 gttgtgggag gtgatgtcca gcttgatgtt gacgttgtag gcgccgggca gctgcacggg
     1441 cttcttggcc ttgtaggtgg tcttgacctc ggcgtcgtag tggccgccgt ccttcagctt
     1501 cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc gctcggagga
     1561 ggcctcccag cccatggtct tcttctgcat tacggggccg tcggagggga agttggtgcc
     1621 gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg gaggagtcct gggtcacggt
     1681 caccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct cggggaagga
     1741 cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg taggccttgg agccgtacat
     1801 gaactgaggg gacaggatgt cccaggcgaa gggcaggggg ccgcccttgg tcaccttcag
     1861 cttggcggtc tgggtgccct cgtaggggcg gccctcgccc tcgccctcga tctcgaactc
     1921 gtggccgttc acggagccct ccatgtgcac cttgaagcgc atgaactcct tgatgatggc
     1981 catgttgtca tcctcgccct tgctcaccat ggtggcgacc ggtaatgtca gacctggtaa
     2041 ctccttactg taacttattg cacctgatga acaaaagatg tgaactgaac gcaagtagga
     2101 ggctaataga ggctagtaca gtacaaagat ccccaaatga tgtgtgccgg ccttagtgga
     2161 ggcagacccc tggttatata cctgtgctgc agaaagaggg gcttacaaat ggatctctct
     2221 ggaatggcat tatctcatgg tgggaacagt gttgtcagca gaatcagcgg ggtggactct
     2281 gacctgagaa tgacagtcta gcactgaatg gctcagacaa tactcttgtg gccaactatc
     2341 accggaaaat aagtgcctgc caaaacaaac cagaccagat gaagctacat ggctgtagaa
     2401 cgtcagtatt taatgtgttg tggccacttg gttcaccaga tttaagagct aattttttta
     2461 aatatcacag ataatcaatc gctttttttt tcttgactga aaagcttaga ctggaaaatt
     2521 ctttgaaaaa gtttgcacta cagaaaataa atacataaca tcctgcactg aatgcacact
     2581 attaatagaa aagagagcat tattgtagga aatgtattgt atatgtacat tctgtgcatt
     2641 tcacaatttt ctcgagcagg aaacagctat gaccatgatt acgccaagct atcaactttg
     2701 tatagaaaag ttgccattat cgtctccctc cagcaccacc cagaaagttg ctcaatgttt
     2761 tgttacagta tgtgtgtaac accttccccc ccagcccagc tctccacttt tctcattcta
     2821 gaaagtacca gtcaactctc caccagccca gctgttggca gacgcacacc tccatccccc
     2881 tgccctcaga catttgccac tgatttctca gctgtcatcc cctctccata aatagaccct
     2941 atcagagaaa gtccattgca ctaatataag gggtgaccac atttctacaa aaccacaatt
     3001 aatgtacaat cacaggaata aacaaacact gcctaaacac acaatatcaa gaacacacaa
     3061 ttacaatctc aggttgtcat tcgcaagctg cgtagttcat tcacaatgaa caggggctta
     3121 aaacaaaagt cattccaccc ctcaccatga aatggattca tccgtaatcc tggtatgtta
     3181 acgctctatg tgtaagatat atataagagt gactcttaaa gtgctgctac tactattgaa
     3241 caaaccaaac gacaagaaac aacactgaag cacagaacag attttctctt acgacgaagc
     3301 tgaattgact tcaatgcaag tttgtacaaa aaagcaggct atgatgggcc gaccggttct
     3361 cgtgcttagt cagaacatca agagagaatc tggacggaag gtccagattg gaaacatctc
     3421 tgcggctaag accatagcag acatcatcag gacatgccta ggaccaaggg ctatgatgaa
     3481 gatgctgctg gatcccatgg gtggtatcgt catgaccaat gatggcaatg ccattttgag
     3541 agagatccag gtccagcatc ctgctgccaa gtccatgatt gagattagcc gcacacagga
     3601 tgaagaagtt ggagatggga ccacctctgt catcattcta gcgggtgaga tgctgtcagt
     3661 ggctgagcag tttttggagc agcagatgca tcctactgtg gtgatcggtg catacagaca
     3721 agctctggat gacatgttga acatactcaa agacataagc actccagtag atgtgagtaa
     3781 tcgagacatg atgttgaaga taataaactc tgcaatcaac accaaagcac tcagccgctg
     3841 gtccacattg gcctgtaaca tcgctctgga tgctgtccgt actgttgagc tggaggaaaa
     3901 tggacgcaaa gagattgaca ttaagaagta tgccaaagtt gaaaaggttc ctggtggcat
     3961 tatcgaagat tcatgcgtgc tgaggggtgt gatggtgaat aaggatgtaa ctcatccccg
     4021 catgcgcaga ctcatcaaga accccagaat tgttctgctg gactgttctc tggagtacaa
     4081 gaagggcgag agccagactg atattgagat tgctcgtgag gaagacttcg cccgcattct
     4141 gcagatggag gaagaatacg ttcagcagat ctgtgaagac atcattcggc tcaaacctga
     4201 cctaatcttc acagagaagg gcatttctga tctggctcag cactatttga tgaaagcaaa
     4261 catcactgct atccgccgta tcaggaagac ggacaacaac cgcattgcaa gagcgtgtgg
     4321 tgcacgtatt gccagcagaa ccgatgagct cactgaagat gatgtgggta caggagcagg
     4381 cctttttgaa gtcaagaaga tcggcgacga gtacttcacc tttgtgacag agtgtaaaga
     4441 ccccggatca ggttcgggaa tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     4501 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4561 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     4621 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     4681 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     4741 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4801 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4861 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     4921 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     4981 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     5041 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     5101 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     5161 ggacgagctg tacaagggat caggtaaagc atgtaccatc ctgctcagag gagccagcaa
     5221 ggaaatcttg gcggaagtgg aacgaaacct acaggatgcc atgcaagtgt gtcgtaatgt
     5281 cctgctggac ccatatctgc tgccgggtgg tggcgcagtg gagatggagg tgtcccatcg
     5341 gctgacggag cgctctcgag ctatgactgg tgtggagcag tggccgtacc gcgctgtagc
     5401 tcaggctctg gaggttgtcc ctcgcacact catccagaac tgtggagctt cagccattcg
     5461 tgtgctcact tctctgaggg ccaagcacac tcaggaggga aactcctcat ggggtgttaa
     5521 tggagagaca ggaactcttg ctgatatgga gcagctggga atctgggaac cgctggctgt
     5581 taaagcccag acttacaaaa ctgcagtgga gacggctatc ctgttactgc gcattgatga
     5641 catcgtttct ggccacaaga agaagggaga tggagagcag acgggtggag caccgatgga
     5701 ggacagagag tagacccagc tttcttgtac aaagtggggg atccagacat gataagatac
     5761 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     5821 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     5881 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttccaac
     5941 tttattatac atagttgata attcactggc cgtcgtttta cggtaccatc gatgatgatc
     6001 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     6061 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     6121 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     6181 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc
     6241 tagatcagat ctgcgaagat acggccacgg gtgctcttga tcctgtggct gattttggac
     6301 tgtgctgctc gcagctgctg atgaatcaca tacttcctcc attttcttcc actgattgac
     6361 tgttataatt tccctaattt ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca
     6421 tgacgtcact tccaaaggac caatgaacat gtctgaccaa tttcatataa tgtgaaaacg
     6481 attttcatag gcagaataaa taacatttaa attaaactgg gcatcagcgc aattcaattg
     6541 gtttggtaat agcaagggaa aatagaatga agtgatctcc aaaaaataag tactttttga
     6601 ctgtaaataa aattgtaagg agtaaaaagt actttttttt ctaaaaaaat gtaattaagt
     6661 aaaagtaaaa gtattgattt ttaattgtac tcaagtaaag taaaaatccc caaaaataat
     6721 acttaagtac agtaatcaag taaaattact caagtacttt acacctctgg ttcttgaccc
     6781 cctaccttca gcaagcccag cagatccact agttctagag cggccgccac cgcggtggag
     6841 ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
     6901 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6961 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7021 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7081 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7141 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7201 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7261 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7321 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7381 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7441 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7501 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7561 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7621 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7681 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     7741 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7801 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7861 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7921 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7981 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8041 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8101 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8161 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8221 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8281 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8341 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8401 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8461 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8521 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8581 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8641 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8701 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8761 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8821 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8881 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8941 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9001 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
//
