LOCUS       sequence-334839-       21551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  alphaA-crystallin promoter (cry) drives GFP expression in the lens. 
            The acta1 muscle-specific promoter drives expression of GFP-tagged 
            zebrafish dmd..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21551)
  AUTHORS   Berger J, Li M, Berger S, Meilak M, Rientjes J, Currie PD
  TITLE     Effect of Ataluren on dystrophin mutations.
  JOURNAL   J Cell Mol Med. 2020 Jun;24(12):6680-6689. doi: 10.1111/jcmm.15319. 
            Epub 2020 Apr 28.
  PUBMED    32343037
REFERENCE   2  (bases 1 to 21551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/jcmm.15319"; journalName: "J Cell Mol Med"; date: 
            "2020-06"; volume: "24"; issue: "12"; pages: "6680-6689"
COMMENT     Sequence Label: pcryGFP-acta1-dmdGFP
FEATURES             Location/Qualifiers
     source          1..21551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    1056..1177
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(1300..2013,2014..2016,2017..2019))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1303..2013,2014..2016,2017..2019))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      2016..2025
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2667..2683
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2703..2723
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(5909..5942)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    6729..6753
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6754..6763
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             12819..12827
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             join(17506..17508,17509..17511,17512..18225)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(17506..17508,17509..17511,17512..18222)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    complement(18225..18249)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    18251..18385
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(18448..18468)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(18476..18492)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    18508..18642
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(19081..19268,19269..19280)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(19314..19330)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(19314..19330)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(19367..19387)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(19367..19385)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(19406..19422)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(19419..19441)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    19430..19446
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(19454..19460,19461..19478,19479..19484))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    19499..19520
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(19637..19654)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(19808..20396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(19888..19907)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(20567..21358,21359..21427))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     21190..21209
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(21428..21532)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggcccgatt ggccttaaga tacattgatg agtttggaca
     1081 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     1141 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     1201 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     1261 atgtggtatg gctgattatg atctagagtc gcggccgctt tacttgtaca gctcgtccat
     1321 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
     1381 gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac
     1441 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
     1501 ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat
     1561 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
     1621 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
     1681 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
     1741 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
     1801 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
     1861 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
     1921 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
     1981 caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg gtaatgtcag
     2041 acctggtaac tccttactgt aacttattgc acctgatgaa caaaagatgt gaactgaacg
     2101 caagtaggag gctaatagag gctagtacag tacaaagatc cccaaatgat gtgtgccggc
     2161 cttagtggag gcagacccct ggttatatac ctgtgctgca gaaagagggg cttacaaatg
     2221 gatctctctg gaatggcatt atctcatggt gggaacagtg ttgtcagcag aatcagcggg
     2281 gtggactctg acctgagaat gacagtctag cactgaatgg ctcagacaat actcttgtgg
     2341 ccaactatca ccggaaaata agtgcctgcc aaaacaaacc agaccagatg aagctacatg
     2401 gctgtagaac gtcagtattt aatgtgttgt ggccacttgg ttcaccagat ttaagagcta
     2461 atttttttaa atatcacaga taatcaatcg cttttttttt tcttgactga aaagcttaga
     2521 ctggaaaatt ctttgaaaaa gtttgcacta cagaaaataa atacataaca tcctgcactg
     2581 aatgcacact attaatagaa aagagagcat tattgtagga aatgtattgt atatgtacat
     2641 tctgtgcatt tcacaatttt ctcgagcagg aaacagctat gaccatgatt acgccaagct
     2701 atcaactttg tatagaaaag ttggcacgcg cagattccgt gtgggcctgg taatatctta
     2761 ccattttaag ctattttaag attagggaaa aaaattaaat aaaactactt taaaattact
     2821 ttccttaaaa agcaacatat ttatttagtt tttgtttaac tggctcaaga ttgtaatatt
     2881 ttacttttac atgttttttt taagtaccta aatgtgttca aaagtaaagt atgttattgt
     2941 tttttttttt ttaatgttag ctaagcttta aattgtaata tattactttt aaaagtgact
     3001 ttttaaaaat gtaaataaaa aagtaactat aaatgtgtaa tgcatgttat ttctgtgggt
     3061 tttttaattt tcatctcaac tgggcttaca attttaacat aataaaaatc aaatcaaatc
     3121 aaatcaggcc cttatttgtc acatacactc ttgtatactg aaataaacta aatgttgaga
     3181 aaaatttaaa tacaccaaaa taacgcatac cttttttgta attacctaag taaaacattt
     3241 agttcctttt ttaagtgact caagattgta atctattaat tgtaaaagat aaaactagtg
     3301 atcaatctga cgtgcttcta tgcagcctgt gagattaatt gtgatgttta attattaaac
     3361 aatttaaatg gatcaaacat ttcacatgtg cttattgtgt ccaacaaaac cagcagaagc
     3421 acttaaaaat attgcatgac aacatgagtt tttaatgtaa attatatata cttcctaaac
     3481 gaagttaatc atattttcta aaataataat gtcattgaaa tatttaaata aatgataasc
     3541 tttagttgat tcaacataaa attgaaagca ataaggactt tttttttttt acagcggtga
     3601 agcaaaagcm tgkaaataat catataaata taataaataa ataaataaaa taatggttct
     3661 tggtgcaaat atgaacactt taaataaata tagaataaat atgatcaata agtcataacg
     3721 acgtgtttta tgttgattat aatttaaaca tgtttctaac gcaagtttta aaaactgttt
     3781 taaagtatta ttttaagtta taagatcaat ttgactcaac attttgtagt gaaaatatgt
     3841 tgtgtaaaat taatataata tacactataa aaatgcaagg ttccacacaa ttcattcatg
     3901 ttgtcccaac acaaatcgat taagttaaca aatatatgtg gattgaacat aaaaaattaa
     3961 gttgtcccaa tgaaatctca agaattgtgt tgtttcagct cattttaaat aagtagtttt
     4021 aacaagtaaa aaataacata tttttgagtg tgtatatata tatatttata tattacaaaa
     4081 tataataata taaaatgtaa aagatataaa ccatgctaca tatatttaag agctattttg
     4141 cacattactt ttttgcaagc catttgagtg tttttttaca aatatatatt agctaaatat
     4201 acaatatctt aaatatacaa ttaatcagac atgcaaaaaa tgcatttaac ctacataaaa
     4261 atacatgatc aacatatgtt ttatgctgat tacaactaaa tcaaacgtaa tgaaattaat
     4321 ttataaaagc gatttaaata tgtcaatatt ttaagttaaa atcacttgaa acctactata
     4381 aatgaactta aatttaaaaa atctccttta attccaatgc agcactgttg ttttttcagc
     4441 tgaggacccc cggtgcagat ggttggcccc cggcccgcta ttctaaggaa gcctttctta
     4501 attttagggc ggcgctgcat gcgctcagat cagattcacg ggtggagagg gttattttct
     4561 cttttttatt ttttcttgcc tgctcatatt tgggcctgtg tgaaccccag gctccgcctc
     4621 ggcccccagg agccgctctt atttaaaggg gatcctcgcc ttcagtcttc gtcccagtgg
     4681 aagcatcagt cttgtgataa caacttggtg agtcctacag accagcgctc gactctaggc
     4741 tttaaatgca ttgatttctc tggattagct accgggagag ttttaacttt gcttattagt
     4801 cttgtgcttg aactgctagg tttaaagagg gttttctcta atgcatgcat gaagcgacag
     4861 ctgaaaacca gaaatagagc gtttgtcctc ctgttaaact ctagcctttc tttggcattt
     4921 gtcagtccga gtcttcagct gccggcagga gattgggttt cagtaacact tgctgagcac
     4981 aaactacacg tgcttccttc agatttaatt cattattaat cattctagat ttaaatgatc
     5041 ttcttactca ttgctgatta ttttagcctt gcggagcaga ttaaatgagc acgtgtttat
     5101 taagattgat ttaaatataa ttaatatttc accactgtag ttttaaaatt gccttctaag
     5161 tcatggttga tttttaatag gtatcggctc taattagatt actattttgg tttttaaatg
     5221 ttatttctat ttcatttgta ggctcgtttt taatgtgatt ttattagatt ctagttttaa
     5281 aatcatcttt tagccttact gagcagactg aatgaacatg tttcattaag attaacttca
     5341 ttatagttaa ttatttaatc attctagtgg attataaatc agctctattt aaattgattg
     5401 tcgtgtttat ttctatttga tttttgatta ttttttaagg tcgtttttat gtgatttttc
     5461 cccgattcta attttaaact aattgtttga tttttatatg attcgactct ttttaaatga
     5521 attgttttgc tttttttatt gagcatatat atatattata caatttcaat caacaattgt
     5581 tgtgcttttt aaatgttgtt tctatttcat ttctagggtc gattttattc gattctagtt
     5641 ttaaaatcgt gatttctaac tcctaacctt actgagcaga ctgaatgaac atgtttcatc
     5701 aagattaact tcattatagt taattattga atcgggctag tggtttataa atcagctcta
     5761 ttataaatta attgtcatca ttatttctat ttaattttta ttatttcttt aggattattt
     5821 ttatgtgatt tttttcacgt tctagtttta aactcaatgc tcattactgc tcacccttgc
     5881 tgagcagtct ttcgttaaga ttaaattaat aacttcgtat agcatacatt atacgaagtt
     5941 attaataacc cattctagtt ttaaaacagt gtcttaaggc atagctgata ttttacaagt
     6001 ttactattta atatatctcc tttctttttt tagggtcgat ttcatctgat ttgattaatt
     6061 tttattatct tcactaagct ttttgttcta ggttttattc tcagtattat tactgttata
     6121 tttgaactga atttctcctc atagtttatt cattttattt atttattttt tgtttattcg
     6181 ctattttgta ttttagtttt taattagaaa ttttattaga atcgttttga actagttttt
     6241 atgcactcta acattaatat taaatcataa ctattatttt tattataatt tatttgtgtt
     6301 tatctttttg tgtagctttc atgtgttttt attttagttt tattatgttt gcattgaatt
     6361 ttttggacta gtttatataa cgatatacag aaaagccatg acaacaaatg tttttataat
     6421 acctattaag tataagctaa tcagatttcc actattctgt tcaaagttaa cattattagc
     6481 cattctgatg gatcatttga catttgtcat atgtctttat gaacactgct ctttcagatg
     6541 ccacttttaa agctgttttt tacagtaaat gaacgtttat tattatgaca ttatttccta
     6601 aggcctctag agtacagcag gatgtggcgt gtgcattacc gtgtaattat gctgactgta
     6661 aatgtgcatt tatagactgt cagcatgtct gactgtgcgc tgttgtgatt ggtcctgcac
     6721 agaccaaggc aagtttgtac aaaaaagcag gctgccgcca tggccgaagc agtgaaccca
     6781 caggaccaat gggaggaagg ttttgaggac gaattcggag aaatcatcaa aactcgatca
     6841 gatgaaagag aagacgttca aaagaaaacc ttcaccaaat ggataaactc acagtttgct
     6901 aagacaagaa gacctcctat tgatgacctc ttcactgacc tgtgtgatgg ccgacgtctt
     6961 cttgaacttc tggaggttct ggttggccat gaaatagtta aagaacgtgg cttcactcga
     7021 gtgcactccc ttaacaatgt taacagggcc ctgcagatcc ttcagaagaa caatgttgat
     7081 ctggtgaaca ttggaggagc tgacattgta gatgggaatc ataaactgac cctggggctc
     7141 atctggagca tcattctcca ctggcaggtt aaggatgtga tgaaagatgt catggcggac
     7201 ttacagcaga ccaacagcga aaagatcctg ttaagctggg tcaggcagtc cctcaaaaac
     7261 taccaagacg tcaacgtggt caacttctcc agcagctggg ccgacggttt cgctttcaac
     7321 gctctcatcc acagccacag gccggagctg ttcagctgga gtgtagtgga gcaacaggat
     7381 aatgccattg agagactgga tcatgccttc ggtgttgcgg agaagagttt aggaatcgac
     7441 cggctgctgg accccgagga tgttgctaca gtccatccag ataagaaatc catcatcatg
     7501 tacgtcacct ccctcttcaa agtgctgccc catggcgtga gtttggaggc catccaagag
     7561 gtggagaccc tccctcgagc cgcagtgacc aaggaggagc acgtcctcta tcaaacccaa
     7621 cagcggtact ctcagcagat cacagtcagc gtggcccaga gtcgcgttcg cagcccgtcg
     7681 ccctcctata agccgagatt taaaagctat gccttcactc aagccgccta cgtcaagaca
     7741 cctgaacagc agaggaagtt cctcatcgca cagtctccag acaaggccga tgaactgcga
     7801 ccgtccccca gtcccctgcc gcaggggcta aatgagctgg aaagctacca gagtgccctg
     7861 gaggaggtcc tgacctggct gctctctgcg gaggacggcc tgcaagcaca gccacccatc
     7921 tcttcttttg tagaagaagt caaagagcag tttcacactc atgagggcta catggtggag
     7981 ctgacgtctc accagggcag tgtgggccgg gtcctgaaag cgggaagtgt gttgttgtct
     8041 ggagggcagc tgactgatga tgaagagagg gaggtgcggg aacagatgaa tctgctcaac
     8101 tcgcgctggg aacatctgcg tgtggccagt atggagcgac agagcaggct tcatgaagtt
     8161 ttgatggacc ttcagcacca gcagctgaag cagctctctg attggctgga cacgacggag
     8221 actcggatta aaaggatggg agcgcaggct ttggggcctg aacttgatga cataaagcgg
     8281 caaatcgaag agcagaaact cttacaggaa gatctggagc tggaacaagt gcgagtgaat
     8341 tcgctgactc atatggtggt ggttgtagat gagaatagtg gggatggcgc caccgcagcc
     8401 ttggaggaaa agctgcagaa ccttggggaa cgatgggcag cgatctgtaa atggactgaa
     8461 gagcgttgga tcctccttca aaagatcctg ctgtgctggc agcatttctc tgaggagcag
     8521 ctgctgtttg attcttggct gactcaaaag gaggagttgg tccagtccat ccaaagcagt
     8581 ggcacaaatg acccaaatga agtagccgcc aacctgcgga agctggcgat tttaaaagca
     8641 gacctggagc tgaagaggca gaccatggac aagctttgct cgctcgtcca ggatctgctg
     8701 actaacatca agagcaaaga ggccgctggg aagctggaag caaagctgga gaggtttgct
     8761 cagcgctggg acaaattggt gcaggcgctt cagctcacca gcaccaagat ttcaactatt
     8821 gtcaccacat cccagtcgga gatcacgcac acaaccatgg caactgtcac caaggtgacc
     8881 acgaaccaga agaagatggt gaagcatact aaggagggca tgtccacccc tccacctcag
     8941 aaaaagagac agattgttgt ggattcagaa ctgcggaaaa gatttgatgt ggacttcact
     9001 gaaattcaca gttttatgac tcggtccgag gctgttttgc aaaatcccga gttctcaatc
     9061 tcccgtaaag aaggcagtgt ggcagatctc tatgaaaaag tgctggcaat tgacagggaa
     9121 aaacccgaaa agttcaggaa gttgcaagaa gccactcgct cagcccaggc tttggtggat
     9181 caacttacca gcgacggtca gaactcagag gacatccagc aggcggctca gcagttacgt
     9241 gctcgatggg tggacttctg cgctctcctg gccgagcggc tggcgtggtt ggcctaccag
     9301 accaaagtgc tggctttcta caatctcttt cagcagctgg agcaggctgc ggccacggcg
     9361 gagaactggc tgaaggtgca gtcgccgccc gcatgtgaac ccgaacctct gaggatccag
     9421 ctggagcgct gcagggatga aatctctcgc ctctcggctc ttcaacctca agtggccaaa
     9481 ctgcacgaac aactcgagga gctgcggcag aaagaagaga cgccggttct gtttgacgcg
     9541 gacatttctg ccttccaaga gcactaccac caagtcctgg aggacctgag ggccagggaa
     9601 cgacagctcg ttttggtcca gtctagtctt cctccagcac gttataagga tgtgatggca
     9661 gcgctgttgg cttggctaca gcagtgtgag aataaactag ccatcccttc tacagcagtg
     9721 actgagtatc ccgtcatgga gcagagactc aaagatatca aggcaatcca agcatcccag
     9781 aaggagcatc agggtgacgt ggatgacctc aataagatgg ctgagcaggt tttccagaaa
     9841 gctccacctg agatctgtca gaagtaccgg acggagttgg ataacgtgat ggtgcgctgg
     9901 agacgcatat cggagcagct ggaggagaac atccagaaac tgcaggatca catgaccaag
     9961 ctacagcagt tccagaatga cacaaagact ctgcagaagt ggatggcaga ggtggatgtt
    10021 ttcctgaatg aggaatggcc agctctggga gacgcagagg ctttggagaa acagttggaa
    10081 cagtgcacgg ctctggtgaa tgacatccac accgtccaac ccagtctgaa cggcatcaat
    10141 gaggtgggac tggccttgaa gagggaagct gagacgccgt ttgccatcaa aatacagaag
    10201 atgcttgatg agctcaatgc gcagtgggag ctcatttgca aacaggctta tgcaaagaaa
    10261 tcagccctga aaggagggct ggacatgacg gtgagtttga ggaaggagat gcaggagatg
    10321 caggagtgga tcactcaggc cgaggaagag tacctggaga gagacttcca gtacaaaaca
    10381 cctgaggagc ttcataaagc agtggaggag ctgaagcggg ctcaagagga agtccatcaa
    10441 aaggaaacaa aggtgaagct gctgacggat aaagtcacca atttcatttc aaaggctcct
    10501 cccgcggccc atgatgccct gaaggcagaa ctggatgttt tgacttctaa ctaccagcgc
    10561 ctgtgcagcc gactcgatgg aaaatgcaag actttagagg aagtgtgggc atgctggtgt
    10621 gagctgctgt cttatttgga gttggagaat gcctggatgg acctacttga aaagaaactt
    10681 gatgaaacgg aagggcttca aggaggcata gaggaaattg aagaagcttt gacttcttta
    10741 gacaccatga ttagagagca tcctgaatac aaccgtaacc agatacgtga attagcgcag
    10801 accctgatgg atggccgagt cctggatgag ctcatccaca aaaaggtgga ggactacaac
    10861 acacgctggg atgaactgat gcaaagggct tcacaaaggc gccagcagct ggagaagagc
    10921 ttgcattggg cccaggagaa cgacaaaacc ctgcgtctca ttcaggactc tctgaacacc
    10981 actgaccgac atctgaccgc ttacatagca gatggcatag acgctgcaca gataccacag
    11041 gaagcacaga aaattcagac tgagttgaac ggccatgagg tgacactgga tgacatgaag
    11101 aaaaaggcta tggaggttga cgcctcagag aaagtgattg gagagatcga tgcaacatta
    11161 gataaacttt tgcaagtaaa gggcaagttc cggcttttcc aaaagccagc aaactttgac
    11221 cagcggctga gggagtgtga gcgagtgctg gaggaggtga aagtgaagct gggagtgctg
    11281 agtattcgca gtgtggagca ggaagtggtg cagtcacagc ttgagcagtg catgaaattt
    11341 tataagaacc tgagtgaggt gaagtctgag gtggagacgg tgataaagac ggggaggcag
    11401 attgtgcaga agcagcagac ggagcaaccc aaggagttag acgacaggct gacggccctc
    11461 aaactcgctt acaatgattt gggttcacag gtaacagaag gaaagcagga actggagaag
    11521 attttgaagt tactgaggaa gtttaggaag gaggtgaaca gtctaaccga atggctggct
    11581 actactgatg gggaactgac gcgccgctca tcggtagagg gtatgccaag tgatttggat
    11641 gcggagttgg cttgggcaaa ggcagcgcag gaggacactg agcatcataa gccacagttg
    11701 aaactggtga gggagttggc agggaccctg aagggtttac ttcatagcca agagaacctg
    11761 attgatgaca aggtcagttt gctgaactgc aactggatcg cagtgacgtc acggagcgaa
    11821 cagtggctca aactcctgct ggattatcaa aatcaaatga aaacacttga gcagaacatt
    11881 tctcagatca acacctggat ggatcgcgca gaagagaagc tggatgagat ggacagccag
    11941 ggctgcattg agcacgttat aaagggtttg cgaatggagt tggaggagat gaaggggaaa
    12001 gttgaagcag tgcaagttct agcagaggat cttataaaga acacagggga acattgtaaa
    12061 gctcaagtga aaccaaagct tgaacagctt aaccaacgtt ttgacattgt tgcaagaagg
    12121 attttaatgg gacaggcctc ctcccaagag ttagacgagt accacagaca ggctaatata
    12181 tggctccagg tgctggacga ggagattaaa ttaggtgaaa gtctaaaaga ggaagatttc
    12241 ctggaggatg cgactataga cgaggaggct ctaaatgaac tgttcctaaa aggggagaat
    12301 ctcctcaaga gaactcccgg tggagagaaa cgagaggccg ttcgagagaa acacaacctt
    12361 ctccatgaca gatatgacac tctaaagaat ctgagagccc tgagaaagaa gaaggctctg
    12421 gctctcgcat cgcagtggta tcagttctgc aagaaaagcg atgacatgat gcagtggctg
    12481 gacagaatcg agaaaagcat acgagagctt ccagatcccc cggaggagcc cagagtgaaa
    12541 gcagttggat atgagattga ccagcagcgg cccaagctgg aggatttacg tggattgggg
    12601 cgtgtgcttt cagagggtgg agctagcaga atggttgagc cccgcctcct gcctatcaat
    12661 aaacgatgga cggagctaga cgttaacttc actcgagtgc gccacaaaag tgagctgcag
    12721 tttctgctgc agtgtatctc tgagaatgag gctctgctga actctcccga gcgctggtct
    12781 actgcattca tcaaccttcc ccaacaagag aagtgcctta aggaggttaa agttaacctg
    12841 gataaactgg agggtcccgt ggcagaggct gttggaagag gtgcatctca acctgaggag
    12901 ggtttgctgg ttcagctgct caggaccaac tgggaaaacc ttaaaatgct ctaccaggac
    12961 agactcaaac gccttgaaaa agccaagaag ttcaatgagg agctgaaaat gctggacaac
    13021 tggctcacag atgctgagag gacgattatg aagtatgaac aagaccccat aaataataga
    13081 gaccacctca aggagcttca ggcgggtttg gagaaacagg aagcagcagt gaagggactc
    13141 aatgctcttg ggactgatct gagtccacag tgcagtaaag acgacaggga ccacattaaa
    13201 cagcagctcg cctcgatcaa ctcccgctgg gccaaagtgt ccaaccagct cactgagatc
    13261 aagagacggt ctgccggggc aaaaatgctt ctcgcagagt taaatgaaga catgggggag
    13321 tttcaatcct ggctggatga tgcggaggct gtggctgcac ttcctgtgga agcgggtcgc
    13381 aaggaacagc tcagtgcaac tttagaaaaa gtcaaggccc gggtggtgga actgccgagc
    13441 aggaagcaag ctcttcatca gatcaacagc aaaggctcat ctctccccgc tgataaagtc
    13501 aaaccattgg agaagcaact aaaggttatc aacatgcgct gggcgaaggt gtccactgat
    13561 cttcctgaga agcagagaca aatcgaggat ctcctcaggg atttgtcttt gtaccaagaa
    13621 cagctctcca aactctcaat ttgggcctcc aatacaaaga ctcagcttga gcagtcgcct
    13681 actgcagtgg acccaaagat tgaggatgac gtaaaagaaa agaagccagc tgtggaaacc
    13741 cttttggcaa aagagagacc gccgtgccag cctgagaagg gccagtatga cggtctgagt
    13801 gcagattgga catccattca agttctcctt aaagactgga aagacaaatg ccagctcgca
    13861 gctgtaactt tgactggtag tgcagcagga gatgcggctc tcgacaaatt caacaaatcc
    13921 tggacagagc ttgacgattg gttggcgctg ctggatcaca tggttcagac ccagagagta
    13981 atggtggggg atctggacga gatcaacgaa atgacggtca aactcaagtc agcactgcaa
    14041 gacatggagc aaagatgccc acagctgaac aaacaaatca ctgcggctca gaacctgaag
    14101 aacaaaacca ataaccctga gacacgggca actattacag accgcattga gaagttacag
    14161 gcacattggg aagattctca tgccaagctg acggccagag ttcttacact gcagaacatg
    14221 tataaggact ccagcgattg gttggaggcc agaaaaaggg tggagcctct cattaagaaa
    14281 gccaatgaaa aactcgagag ctggaagaaa gtttcgcaca gcgttgaaga tttaaaaggc
    14341 caaaatgcag atgtcaagca actttctaag gatctacagc agtggcagac tcagatgaat
    14401 gtcaccaatg agctggcaaa caaactactg actctttatg cagacgacga cacaagcaaa
    14461 gtgaaacaaa tgaccgagag catgaatctg gcttgggcca acatcaaaaa gcgtgcaggg
    14521 gacaaggagg cagatctgga agctggactt cggcagttgc agcattacta cttggacctg
    14581 gagaaattcc ttaattggct aacggaggca gaaaccacag ccaatgtctt acaagatgcc
    14641 acctttaagg agggacttct ggaaaatcct gccacagttc gacatttact cgagcaatgg
    14701 caggatctcc aggcagagat tgatgctcac cgggagacgt accattcatt agatgaaaac
    14761 ggacatcgta ttgtgtcgtc tctggaggga acggacaatg ctgtggtgct acaaaaacgg
    14821 cttgatgaca tggggcagcg ctggcatgag ttgtgcaaca aagttatgag tataaggccc
    14881 tatctagatg ccggcgttga tcagtggaaa cacttgcaca tgtccttaca agagctgctc
    14941 aactggctgc agctgaagag ggaagagctg gagaagcaga agccagtagg gggcgacgtg
    15001 ccgaccgttc accaacaact cctcacgcac aaggctttca gaagagagct gggtgccaaa
    15061 gaacctgtaa tcaatggaac tcttgacaat gcgaaaacct tcctcgctga gatgcctcgt
    15121 gagggcctga aacagagacc tggacaaaaa gatgtaagtc ctgaggagcg ggttcaaaac
    15181 gtgggccgca tattgcgtaa agaagtggag gatgtaacgg tgcgatggaa gaatctgggt
    15241 gcagcttctg tggactggca gcaacagctg gaactggcct tggagaggct gatggagctc
    15301 caggacgccc aggatcagct ggactacaag ctacgacagg ctgagagcgt gaagaattct
    15361 tggaagcctg ttggggagct gcttgtagac gatttgcaaa accacataga cagagtgaag
    15421 gcatttcagg aagagatagc tcccattcag gataacgtaa atcatgtcaa ccagctggcc
    15481 tccacattta gaccgtctga cattcagctt tctccagaca acctgagcag aatcgatgac
    15541 ctcaacatga gatggaggct tctgcagatc tccattgagg agcatctgag tcagctgacc
    15601 acagccttta aggacttggg accctctcag aatttccttc atgcgtctgt tgaaagccct
    15661 ctcgaacgct ctatttcacc caacaatgtc ccctactata tcaatcacca gacccaaaca
    15721 acatgttggg accacccaaa gatggcagaa ctctaccagt cattagcgga tctcaacaac
    15781 gtgcggttct cggcatacag gacggcaatg aagctcagac gaatgcagaa agccctctgt
    15841 ttggatcttc tgagcatgcc tgcagcctgt gaagcctttg agcagcacaa tctcaaacag
    15901 aacgagcagt tcatggacat cgtgcaggtg atcaactgtc tgaccagcat ctacgaccgt
    15961 ctggagcagc agcacagcag cctggtcaac gtgcctctct gtgtggacat gtgtctcaac
    16021 tggctgctca acgtttacga tacgggacga gctgggaaga ttcgtaccct atccttcaaa
    16081 acaggaataa tctctttgtg caaagctcac cttgaagata agtacagatt tttatttcga
    16141 gaggtggcca gtgccacagg cttctgtgac cagcggcgcc tcggcctcct cctgcatgat
    16201 gccattcaga tccccaggca gctgggtgaa gtggcgtctt tcggagggag caatattgag
    16261 cccagtgtgc gcagctgctt tcagttcgcc aataacaaac cggagttaga ggcttcagtc
    16321 ttcctggact ggatgcgttt agaacctcag tcgatggttt ggcttcctgt ccttcaccgt
    16381 gtagcggccg ctgagacagc aaagcaccag gctaagtgca acatttgtaa ggaatgtcct
    16441 attattggct tcaggtaccg aagtttaaag cactttaact atgatatctg ccaaagctgc
    16501 ttcttttctg gcagagtggc caaaggtcac aagatgcagt accctatggt tgaatattgc
    16561 acaccgacga cgtcaggaga ggatgtgaga gactttgcca aggtgttaaa gaacaagttc
    16621 aggacaaagc gctattttgc caagcaccct cgcatgggtt accttcccgt ccagaccatc
    16681 cttgaaggag acaacatgga gactcctgtt acactgatca acttctggcc tgttgaccat
    16741 ccgcctgcat cttcccctca actctcccat gacgacaccc actctcgcat cgagcattac
    16801 gctagcaggc tagctgaaat ggagaaccga aatggctcgt atgtgaatga caatgtatcg
    16861 cccaatgaaa gcatggatga tgagcatctg ctgatccagc actactgcca gagtctgaat
    16921 caaggctctc ctctcagcca gccgcagagc cccgctcaga tcctcatctc aatggagact
    16981 gaagagaagg gagagctgga gagagtgctc aatgatctgg agcaggaaaa caggaagctg
    17041 caagcggagt atgatcgtct gaaaaaggca cacgatcaca agggtctgtc accgttgcct
    17101 tcacctccac agatgcttcc ggtgtcgcct cagagtccac gcgacgcaga actgatcgca
    17161 gaagccaaac tactgcggca acacaaggga cgattggagg caagaatgca aatcctggag
    17221 gatcacaaca agcagctgga atcgcagctt acacgcctaa gacagttact cgagcagact
    17281 gagtccaagg tgaatggcac tgccctgtcc tcaccctcca ctgcctctcc gagatctgac
    17341 accagcctgg cctcactgcg tgtggccgca agccaaacca cagagaccat gggtgatgat
    17401 gagctgtcca gtcccaccca ggatgcaagc actggattag aagacgtcat cgagcagctc
    17461 aacaactcct tccctcacag ccaaggaggc ggacggctaa atccaatggt gagcaagggc
    17521 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
    17581 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcta cctacggcaa gctgaccctg
    17641 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
    17701 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
    17761 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
    17821 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
    17881 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
    17941 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
    18001 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
    18061 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
    18121 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
    18181 accgccgccg ggatcactct cggcatggac gagctgtaca agtaacccag ctttcttgta
    18241 caaagtgggg gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
    18301 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
    18361 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
    18421 tcagggggag gtgtgggagg ttttttccaa ctttattata catagttgat aattcactgg
    18481 ccgtcgtttt acggtaccat cgatgatgat ccagacatga taagatacat tgatgagttt
    18541 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
    18601 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
    18661 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
    18721 tacaaatgtg gtatggctga ttatgatcct ctagatcaga tctgcgaaga tacggccacg
    18781 ggtgctcttg atcctgtggc tgattttgga ctgtgctgct cgcagctgct gatgaatcac
    18841 atacttcctc cattttcttc cactgattga ctgttataat ttccctaatt tccaggtcaa
    18901 ggtgctgtgc attgtggtaa tagatgtgac atgacgtcac ttccaaagga ccaatgaaca
    18961 tgtctgacca atttcatata atgtgaaaac gattttcata ggcagaataa ataacattta
    19021 aattaaactg ggcatcagcg caattcaatt ggtttggtaa tagcaaggga aaatagaatg
    19081 aagtgatctc caaaaaataa gtactttttg actgtaaata aaattgtaag gagtaaaaag
    19141 tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt tttaattgta
    19201 ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa gtaaaattac
    19261 tcaagtactt tacacctctg gttcttgacc ccctaccttc agcaagccca gcagatccac
    19321 tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
    19381 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
    19441 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    19501 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
    19561 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    19621 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    19681 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    19741 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    19801 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    19861 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    19921 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    19981 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    20041 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    20101 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    20161 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    20221 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
    20281 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    20341 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    20401 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    20461 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    20521 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    20581 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    20641 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    20701 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    20761 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    20821 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    20881 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    20941 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    21001 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    21061 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    21121 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    21181 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    21241 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    21301 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    21361 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    21421 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    21481 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    21541 cccgaaaagt g
//
