LOCUS       sequence-334849-       12719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion
            (lipid droplet reporter) with cmlc2:eGFP as a transgenesis marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12719)
  AUTHORS   Lumaquin D, Johns E, Montal E, Weiss JM, Ola D, Abuhashem A, White 
            RM
  TITLE     An in vivo reporter for tracking lipid droplet dynamics in 
            transparent zebrafish.
  JOURNAL   Elife. 2021 Jun 11;10. pii: 64744. doi: 10.7554/eLife.64744.
  PUBMED    34114952
REFERENCE   2  (bases 1 to 12719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-06-11"; volume: "10. pii: 64744. doi"; pages: " 
            10.7554/eLife.64744"
COMMENT     Sequence Label: pDestTol2CG2--3.5ubb:plin2-tdtomato
FEATURES             Location/Qualifiers
     source          1..12719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(622..633,634..796)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1169..1185
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1205..1225
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    4760..4784
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6137..7567
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    complement(7588..7612)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    7614..7748
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7811..7831)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(7839..7855)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    7871..8005
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(8307..9020,9021..9023,9024..9026))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8310..9020,9021..9023,9024..9026))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8352..8373)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8701..8720
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8960..8981
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(10249..10436,10437..10448)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(10482..10498)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10482..10498)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(10535..10555)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10535..10553)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10574..10590)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10587..10609)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10598..10614
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10622..10628,10629..10646,10647..10652))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10667..10688
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10805..10822)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10976..11564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11056..11075)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11735..12526,12527..12595))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12358..12377
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12596..12700)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccgggctg gctaagaact catcagcctc
      361 cccggtccat ctacccacgt accaatgcac caattggcca caatgacggc tactacatgg
      421 tgccattcct tcctctttat aggaatggag actacctcct gtccaacaag gctcttggat
      481 acgagtacgc ctacctgttg gacccaggtc attgcacaac accagaaatg ccctctgatc
      541 tgcaaaagac gtgaatatct gttcagacac ccatatccac tctgttccac acaggtcaga
      601 ggtttgtcca ggagttcttg acagaggtgt aaaaagtact caaaaatttt actcaagtga
      661 aagtacaagt acttagggaa aattttactc aattaaaagt aaaagtatct ggctagaatc
      721 ttacttgagt aaaagtaaaa aagtactcca ttaaaattgt acttgagtat taaggaagta
      781 aaagtaaaag caagaaagaa aactagagat tcttgtttaa gcttttaatc tcaaaaaaca
      841 ttaaatgaaa tgcatacaag gttttatcct gctttagaac tgtttgtatt taattatcaa
      901 actataagac agacaatcta atgccagtac acgctactca aagttgtaaa acctcagatt
      961 taacttcagt agaagctgat tctcaaaatt gttagtgtca agcctagctc ttttggggct
     1021 gaaaagcaat cctgcagtgc tgaaaagcct ctcacaggca gccgatgcgg gaagaggtgt
     1081 attagtcttg atagagaggc tgcaaatagc aggaaacgtg agcagagact ccctggtgtc
     1141 tgaaacacag gccagatggg ccctcgagca ggaaacagct atgaccatga ttacgccaag
     1201 ctatcaactt tgtatagaaa agttggctcc gaattcgccc ttaaaactcg agaccagcaa
     1261 agttctagaa tttgtcgaaa catttatgtt atatatttcc tgaaaaaaat tctgagtaag
     1321 ttcttaagtg ttattgccag caacataaac aacagacggc aaaatgaata aatgataaca
     1381 aagcagtagg cttaaataaa cctaattttt ataggctgtt ctctacaacc ctcaaacagt
     1441 gattagtttt gtacttataa acttgccctt tcattcatat ttcaagaaaa ttggttcaga
     1501 agatctggat attctagcag ttgttcaagc tcatggaggg atcagtgacc tgattccaca
     1561 atgactaggc ctaatccaga aattagatga ctgtcaacat aaaaaggcac agcactcact
     1621 agctgcccta tatattttat tatattttac atatattatt ttatttattt agctctgagt
     1681 gctgtacttt ctggttaaag aaaactgctt acaacagcta acctgtacta cctcaggctc
     1741 agggaatttg gaacaggttt gtctggtttg tttctttaac catgcatgct tgttttcaac
     1801 tatggcaaca cagtcacatg ggacattaca gaaatgattt gtcgatgaca tgcgactttt
     1861 ctttaataaa gcgcaaagat cccaaaaagc aaacttttaa caaaaatcat ataattatat
     1921 tttcaatcca gctttgtagc aactttgtgc tgctgttcac tcagcaacag atagtcagta
     1981 taaggtcagt gtgtctcaaa gcagtgccat ctgtttcaca cattgcgttc tatatataag
     2041 tgtgctggtt gacacgacac tgtataaggc ctaggctaaa acacaaacaa tgtagaatga
     2101 cactgtgttt tttttgtaaa caaatgttgt ttttggttaa acatctttgt gaaaacatcc
     2161 tcctgtcatg tatttgctat attcaaatgt taaacccgtg cagaatagaa catatacaaa
     2221 aaaaaacaac acaacacatt tttaaacatt attaaatatc aagtattgct ggcagttctg
     2281 tttctgtttt acagtaccct ttgccacagt tctccgcttt tcctggtcca gattccacaa
     2341 gtctgattca ccaatagcaa agcgaataaa caaccaaagc agccaatcac tgcttgtaga
     2401 ctgtcctgcg agaccggccc attccagcac attctggaaa cttcctttat atgataatta
     2461 taaatacatt taaattattg atacaaaaca tgtaattcct agaacataac catagcaatc
     2521 attagttttc agggtaatta tgtattttta ggatttgact gcggaaagat ctggtcatgt
     2581 gacgtctcat gaacgtcacg gccctgggtt tctataaata cagtaggact ctcgaccatc
     2641 ggcagatttt tcgaagaaga agatcagttt caggagccgt actgttccgt ttcaacgcaa
     2701 atattaacgg taagagcgaa tttcctagtt tgttttcatg ccattcttta aaaccatagc
     2761 gtattacttt aattatagta aactttcgct ttctttatta caagagacgt tttgtgttga
     2821 ttctccgcgg acattttcgg tcagacaatc agaaaatgac cgcggaggac cagtaacttg
     2881 cattacacgt aagttaaatc ttcgtgtatt aaaatggtta ggttgttaac gtcaaatagg
     2941 ttaccgtgtt tgcgtgtgat caggttggtt ttgttagatt tttgtcagta tttttaattt
     3001 atttgtttta gtttatttat tttttttgct gaatcatagt ttgtgaacaa agaacccgga
     3061 tgttacatac agtacagccg ccatgttaca gagagttata acttaatcat tttaaaaata
     3121 attttgcctt acttttagtt tgtcatgttg agaaatgagg aaatgttaaa atgaggaaat
     3181 atccaattaa tttaatatat caaaataatc catgattaca atgcactgaa ctggagaaaa
     3241 ttaagatgtt ttctagtgtc atgaaacaaa tgtaagagat gtacattgta gatgttttat
     3301 gtcaagaatt ggctagttga tgcagcatac tggcgatact cagttgtaat aacagtaacg
     3361 ttacatgtta atagactact gagtatgctg ttctgtctat gtatgctctg taagctacga
     3421 gaaggacttt tttaaacagt aaagggtgca atatttttac aaattgaatt aaataaaggc
     3481 tgtctattaa gtaatatgct tgatattttt cttacttgat cggaaataag aaaaaatata
     3541 aacgttgttg ctctaaaaat cctagttcag tttagccaac cacaaatacc tttttgttcc
     3601 tccaacagtt tttttttctt ctctataata tttggcagtc tatagtactc caaatgtttc
     3661 cccacagtct aactaattgg tacagccaaa atcatgacac ttattgcaat aataattttg
     3721 gttcattggc attgttgata gcctgtgcca ctaatatggt cgattgatca tgcttcagga
     3781 agaaaactat attgtttgat gtaagattat taaatcttca cctgcttcca ttacaaacta
     3841 ttcccatctt attgaattct ggtatgtctt aaaggattag ttcacttccc aaatcaaaat
     3901 ttacttagcc ttttttcatc catgatccct ttttttcatc attaatgaag aaattgtttt
     3961 tgaaaaagtt tcaagatttt tttctctata ttgtggagct tgttagttta aaattccaaa
     4021 atgcaatatg tggcttcaaa tggttctaaa tgatcccagt caaggaataa cagtcttatc
     4081 taatgaaacc attagacctt taaaaataaa aaaaataaaa gtatttattt ttaaatgact
     4141 gagtgattaa gttgaatttc agcgtttcct tactgtgtag aagtccttcc ttactggccc
     4201 caccctttgg ttctctgcca atctgctacc taatgtaatg ttgtggaaca ttattattct
     4261 ttatttctta attttttatt ttttatttta aaacaatgta aactgcacag atgtgcagtt
     4321 tgtttaaaat ggccaatgct ttggaaatgc atgacataat tagatttcat gatgcacaaa
     4381 gccaaatctc agagcttgtg caaaatgagc tatcatttca ctaggtaaga ccctaaattt
     4441 tcatatagga tcatttggac aattttgctg caggtaaaat gcattctata gtccactgtc
     4501 agccattgtt ttggatagta tttatttttc tctacaagta tagtcaatag ttttctatta
     4561 ttttaaaggt ttgtaacatt taagggtgac caaatgcaaa gtaaaatttc attttcgggt
     4621 gaactatctc gtttaacatg ggagaagtgc aaaacataca ttattggcta gaacattgta
     4681 gtatttttta aatggaaatg tgtgattgct aatcttactt tgaatttgtt tacagggatc
     4741 caaaaagggc gaattcgacc caagtttgta caaaaaagca ggctccgcgg ccgccccctt
     4801 caccatgagc tttcttctgt acttgaaact gcacaacaga ttttctgcta acatgggttc
     4861 tatggaggat gtaaataacc agaatgtggt tgcccgaatt gttcaccttc ccctggttgt
     4921 ttccacctat gacatgatgt cgaacgtgta cgtcaacacc aagaacaacc acccatacct
     4981 gaagtctgtg tgcgaggttg ctgagaatgg tgtcaaatcc ataacctcag ctgcctttca
     5041 caggtcttta ccaatcattg gcaaactgga gcctcagatc tctatggcaa atgacctggc
     5101 gtgcaaagga ctggataaga tagaaaagac tctgcccatc ctacacaagc catctgaaca
     5161 gattatggcc aatgctaaag atgcagtgac aggagccaag gaggctgtca gcgggactgt
     5221 gagcggtgcc aaggattctg tctccttcac cctaaatgat gtgatggacc gaacacgcgg
     5281 ggcagtgcag ggcagcgtgg agaagaccaa gactgtggtc agcggtggag tccagacagt
     5341 gatggacagc aggatgctca aactggtgtc cagcagagtg gacaatgcac tgagcacatc
     5401 tgagacgctg ctggagcact acttgcctga aaatgaggag gaaaaagaag gtgaagagag
     5461 gaatactgaa ggattggaaa atgacagcga tttggccaat tattatgtcc gactgggctc
     5521 tctgtccatt aaagtcagag acaggactta tcagagagcc gtggagaaag ttcgtcatgc
     5581 cgtgcagcgc ggacaggagt ccatttcaca gcttaaccac acaatggata taatcgaatt
     5641 taccaggaag aatattgatg ggtctaacca gaagctgcag gagaaactgg gttcactaat
     5701 gggctggaag agcgtttctc aaagccaaga aacagagagc gaaacagaca gtgaagctga
     5761 gcaaattgag tcgcgcacac tgaccatcgc ccacagcctc agccagcagc ttcagagcac
     5821 atgtgtggtg ctggtgtcca gtctcagggg tctccctcag cacgtccagc tccaggtggg
     5881 ctccgtcagc cgctccgcat tggagatcta cagcagcttc agtaaagcgg ccgtgctggg
     5941 ggatctgtcc agcaccgtcc tgtcctccag ccgctcacag ctcagcagaa tcacagagtc
     6001 catggacaac gtgatggact acctgctcaa caacactcct ctcaactggc tggtcggacc
     6061 gttttaccca cgtgtggagc gcgccgttga aaacgaggcc aaatgtagca aacagaggac
     6121 ccttcaagtc actgaaatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt
     6181 caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga
     6241 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct
     6301 gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa
     6361 gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga
     6421 gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca
     6481 ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc
     6541 cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga
     6601 cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct
     6661 ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta
     6721 cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     6781 cgagcgctcc gagggccgcc accacctgtt cctggggcat ggcaccggca gcaccggcag
     6841 cggcagctcc ggcaccgcct cctccgagga caacaacatg gccgtcatca aagagttcat
     6901 gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga
     6961 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
     7021 ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta
     7081 cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa
     7141 gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc
     7201 cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga
     7261 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc
     7321 ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca
     7381 ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta
     7441 ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     7501 acagtacgag cgctccgagg gccgccacca cctgttcctg tacggcatgg acgagctgta
     7561 caagtagaag agcccgggcg atcgcgaacc cagctttctt gtacaaagtg ggggatccag
     7621 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     7681 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     7741 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     7801 aggttttttc caactttatt atacatagtt gataattcac tggccgtcgt tttacggtac
     7861 catcgatgat gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     7921 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     7981 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     8041 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     8101 tgattatgat cctctagatc agatctcttg tttattgcag cttataatgg ttacaaataa
     8161 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8221 ttgtccaaac tcatcaatgt atcttatcat gtctggatct acgtaatacg actcactata
     8281 gttctagagg ctcgagaggg gccgctttac ttgtacagct cgtccatgcc gagagtgatc
     8341 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
     8401 ctcagggcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
     8461 atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg
     8521 cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg
     8581 tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
     8641 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
     8701 gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc
     8761 atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc
     8821 acgccgtagg tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg
     8881 cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg
     8941 ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg
     9001 aacagctcct cgcccttgct caccatggtc actgtctgct ttgctgttgg tctgggctcc
     9061 tgggtcactg gcttactaat ggagtcttta tgtatgagga ctcttatcaa ttgttcttct
     9121 ataaaggtct gcagtgtttc tgttcgtccc ctacatggac acccagagcc tcctaaatac
     9181 aggagccctg ataactgcac aagtgctcag attccagcag ggtggaaaat gagataaagt
     9241 gtgcagatgg ggagggggac gtgaatgaga gatttgaggg atgaaaagga tggatgaacg
     9301 cattgaaaat agcccctttc acaaaataat accagtaaat tgtcatataa ttaatgacca
     9361 gacctttacc tgtaaatgat gtgatgttca tatacagaag aatgccaaca atttacagat
     9421 gattttacaa attcttaatt ttccttttct aaatggattt gttcacacat tatctcttca
     9481 aagcaattga attttctgga aaagattggc tgtgtgtgta aaaggtgcta ctgaggaatt
     9541 taatgtgaca tggaatgaag caaacagcag cctaagacag gatggcagga aaaatgtcac
     9601 atgtttaaaa taagagtgaa accaacgacc tggacttata gagactgtgt gtccttaggc
     9661 aaagctcttg tacaaacagt gcttgtgact gtgaaattcg gggtttgcct ggattgtgtt
     9721 aaatgtgtgc ttggatgtat cataaaaaag aatcggttct ctgtatttga cattttctgt
     9781 aatttatcta aagttacaac tggctatgcc tgatttttat cactattgaa tcaatcattt
     9841 taaagaatgt taagtctgct gatttggaga gctcatttac atttattttg aatgtctctt
     9901 atttaacaca actgatttaa gctttagatc tgcgaagata cggccacggg tgctcttgat
     9961 cctgtggctg attttggact gtgctgctcg cagctgctga tgaatcacat acttcctcca
    10021 ttttcttcca ctgattgact gttataattt ccctaatttc caggtcaagg tgctgtgcat
    10081 tgtggtaata gatgtgacat gacgtcactt ccaaaggacc aatgaacatg tctgaccaat
    10141 ttcatataat gtgaaaacga ttttcatagg cagaataaat aacatttaaa ttaaactggg
    10201 catcagcgca attcaattgg tttggtaata gcaagggaaa atagaatgaa gtgatctcca
    10261 aaaaataagt actttttgac tgtaaataaa attgtaagga gtaaaaagta cttttttttc
    10321 taaaaaaatg taattaagta aaagtaaaag tattgatttt taattgtact caagtaaagt
    10381 aaaaatcccc aaaaataata cttaagtaca gtaatcaagt aaaattactc aagtacttta
    10441 cacctctggt tcttgacccc ctaccttcag caagcccagc agatccacta gttctagagc
    10501 ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct
    10561 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
    10621 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
    10681 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
    10741 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    10801 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    10861 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    10921 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    10981 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    11041 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    11101 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    11161 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    11221 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    11281 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    11341 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    11401 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    11461 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    11521 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    11581 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    11641 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    11701 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    11761 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    11821 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    11881 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    11941 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    12001 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    12061 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    12121 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    12181 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    12241 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    12301 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    12361 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    12421 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    12481 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    12541 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    12601 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    12661 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtg
//
