LOCUS       sequence-334850-        8104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression construct of GFP tagged full length DAXX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Bouchard JJ, Otero JH, Scott DC, Szulc E, Martin EW, Sabri N, 
            Granata D, Marzahn MR, Lindorff-Larsen K, Salvatella X, Schulman BA,
            Mittag T
  TITLE     Cancer Mutations of the Tumor Suppressor SPOP Disrupt the Formation 
            of Active, Phase-Separated Compartments.
  JOURNAL   Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi: 
            10.1016/j.molcel.2018.08.027. Epub 2018 Sep 20.
  PUBMED    30244836
REFERENCE   2  (bases 1 to 8104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.08.027"; journalName: "Mol Cell"; date: 
            "2018-10-4- 4"; volume: "72"; issue: "1"; pages: "19-36.e8"
COMMENT     Sequence Label: pcDNA6.2EmGFP-DAXX
FEATURES             Location/Qualifiers
     source          1..8104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..172
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    230..254
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2484..2508)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2516..2557
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(2597..2616)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2641..2689
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      2891..3319
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2978..2997)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3188..3209
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3328..3348)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3333..3662
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3513..3648
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3575..3594
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3710..3757
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3776..4174
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4332..4453
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4369..4388)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4423..4442
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4502..4518)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4502..4518)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4515..4537)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4526..4542
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4550..4556,4557..4574,4575..4580))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4595..4616
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4733..4750)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4904..5492)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4984..5003)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5663..6454,6455..6523))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6286..6305
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6524..6628)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6703..6722)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6894..7273
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7274..7477
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7428..7448
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7474..7498
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(7602..7604,7605..7607,7608..8104,1..214)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7647..7668)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7908..7927)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
       61 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
      121 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      181 cgggatcact ctcggcatgg acgagctgta caagggctcg agcccatcaa caagtttgta
      241 caaaaaagca ggctccacca tggccaccgc taacagcatc atcgtgctgg atgatgatga
      301 cgaagatgaa gcagctgctc agccagggcc ctcccaccca ctccccaatg cggcctcacc
      361 tggggcagaa gcccctagct cctctgagcc tcatggggcc agaggaagca gtagttcggg
      421 cggcaagaaa tgctacaagc tggagaatga gaagctgttc gaagagttcc ttgaactttg
      481 taagatgcag acagcagacc accctgaggt ggtcccattc ctctataacc ggcagcaacg
      541 tgcccactct ctgtttttgg cctcggcgga gttctgcaac atcctctcta gggtcctgtc
      601 tcgggcccgg agccggccag ccaagctcta tgtctacatc aatgagctct gcactgttct
      661 caaggcccac tcagccaaaa agaagctgaa cttggcccct gccgccacca cctccaatga
      721 gccctctggg aataaccctc ccacacacct ctccttggac cccacaaatg ctgaaaacac
      781 tgcctctcag tctccaagga cccgtggttc ccggcggcag atccagcgtt tggagcagct
      841 gctggcgctc tatgtggcag agatccggcg gctgcaggaa aaggagttgg atctctcaga
      901 attggatgac ccagactccg catacctgca ggaggcacgg ttgaagcgta agctgatccg
      961 cctctttggg cgactatgtg agctgaaaga ctgctcttca ctgaccggcc gtgtcataga
     1021 gcagcgcatc ccctaccgtg gcacccgcta cccagaggtt aacaggcgca ttgagcggct
     1081 catcaacaag ccagggcctg ataccttccc tgactatggg gatgtgcttc gggctgtaga
     1141 gaaggcagct gcccgacaca gccttggcct cccccgacag cagctccagc tcatggctca
     1201 ggatgccttc cgagatgtgg gcatcaggtt acaggagcga cgtcacctcg atctcatcta
     1261 caactttggc tgccacctca cagatgacta taggccaggc gttgaccctg cactatcaga
     1321 tcctgtgttg gcccggcgcc ttcgggaaaa ccggagtttg gccatgagtc ggctggatga
     1381 ggtcatctcc aaatatgcaa tgttgcaaga caaaagtgag gagggcgaga gaaaaaagag
     1441 aagagctcgg ctccaaggca cctcttccca ctctgcagac acccccgaag cctccttgga
     1501 ttctggtgag ggccctagtg gaatggcatc ccaggggtgc ccttctgcct ccagagctga
     1561 gacagatgac gaagacgatg aggagagtga tgaggaagag gaggaggagg aggaagaaga
     1621 agaggaggag gccacagatt ctgaagagga ggaggatctg gaacagatgc aggagggtca
     1681 ggaggatgat gaagaggagg acgaagagga agaagcagca gcaggtaaag atggagacaa
     1741 gagccccatg tcctcactac agatctccaa tgaaaagaac ctggaacctg gcaaacagat
     1801 cagcagatct tcaggggagc agcaaaacaa aggacgcata gtgtcaccat cgttactgtc
     1861 agaagaaccc ctggccccct ccagcataga tgctgaaagc aatggagaac agcctgagga
     1921 gctgaccctg gaggaagaaa gccctgtgtc tcagctcttt gagctagaga ttgaagcttt
     1981 gcccctggat accccttcct ctgtggagac ggacatttcc tcttccagga agcaatcaga
     2041 ggagcccttc accactgtct tagagaatgg agcaggcatg gtctcttcta cttccttcaa
     2101 tggaggcgtc tctcctcaca actggggaga ttctggtccc ccctgcaaaa aatctcggaa
     2161 ggagaagaag caaacaggat cagggccatt aggaaacagc tatgtggaaa ggcaaaggtc
     2221 agtgcatgag aagaatggga aaaagatatg taccctgccc agcccacctt cccccttggc
     2281 ttccttggcc ccagttgctg attcctccac gagggtggac tctcccagcc atggcctggt
     2341 gaccagctcc ctctgcatcc cttctccagc ccggctgtcc caaacccccc attcacagcc
     2401 tcctcggcct ggtacttgca agacaagtgt ggccacacaa tgcgatccag aagagatcat
     2461 cgtgctctca gactctgatt tggacccagc tttcttgtac aaagtggttg ataacgggaa
     2521 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgagttta
     2581 aacgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     2641 cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg
     2701 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     2761 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     2821 gcagccaacg tcggggcggc aggccctgcc atagcagatc tgcgcagctg gggctctagg
     2881 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     2941 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3001 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     3061 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     3121 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     3181 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     3241 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     3301 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     3361 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     3421 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3481 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3541 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     3601 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     3661 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
     3721 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
     3781 caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag
     3841 catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat
     3901 cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct
     3961 gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga
     4021 gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc
     4081 tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga
     4141 attgctgccc tctggttatg tgtgggaggg ctaagcactt cgtggccgag gagcaggact
     4201 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     4261 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     4321 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4381 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4441 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     4501 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4561 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4621 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4681 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4741 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4801 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4861 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4921 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4981 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     5041 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5101 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5161 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5221 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5281 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5341 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5401 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5461 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5521 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5581 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5641 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     5701 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5761 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5821 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5881 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5941 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     6001 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     6061 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6121 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6181 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     6241 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     6301 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     6361 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     6421 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6481 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6541 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     6601 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     6661 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     6721 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     6781 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     6841 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     6901 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     6961 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     7021 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     7081 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     7141 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     7201 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     7261 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     7321 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     7381 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     7441 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     7501 ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct
     7561 ccggactcta gaggatccct accggtgata tcctcgagac catggtgagc aagggcgagg
     7621 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     7681 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     7741 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcacct
     7801 acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     7861 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     7921 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     7981 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     8041 acagccacaa ggtctatatc accgccgaca agcagaagaa cggcatcaag gtgaacttca
     8101 agac
//
