LOCUS       sequence-33486-e         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Aoyama M, Agari K, Sun-Wada GH, Futai M, Wada Y
  TITLE     Simple and straightforward construction of a mouse gene targeting 
            vector using in vitro transposition reactions.
  JOURNAL   Nucleic Acids Res. 2005  . 33(5):e52.
  PUBMED    15784610
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2005  . 33(5):e52."
COMMENT     Sequence Label: pGPS21loxFRTNeo
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..29
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    121..168
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    complement(185..218)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     mobile_element  231..396
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gtgcccagcg gggctgctaa agcgcatgct ccagactgcc ttgggaaaag cgcctcccct
       61 acccggtaga attaattcct cgatcgagtc tagaggagca tgcggcggcc atcaagcttt
      121 gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcag aaagcttcat
      181 ggagataact tcgtatagca tacattatac gaagttatgt cgacctcgac aaccagataa
      241 gtgaaatcta gttccaaact attttgtcat ttttaatttt cgtattagct tacgacgcta
      301 cacccagttc ccatctattt tgtcactctt ccctaaataa tccttaaaaa ctccatttcc
      361 acccctccca gttcccaact attttgtccg cccacaccgt aagatgcttt tctgtgactg
      421 gtaccctgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc
      481 cagcagccgc acgcggcgca tctcgggcag cgttgggtcc tggccacggg tgcgcatgat
      541 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa
      601 tgaatcaccg atacg
//
