LOCUS       sequence-33487-e         648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Aoyama M, Agari K, Sun-Wada GH, Futai M, Wada Y
  TITLE     Simple and straightforward construction of a mouse gene targeting 
            vector using in vitro transposition reactions.
  JOURNAL   Nucleic Acids Res. 2005  . 33(5):e52.
  PUBMED    15784610
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2005  . 33(5):e52."
COMMENT     Sequence Label: pGPS21loxFRTNeo
FEATURES             Location/Qualifiers
     source          1..648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    178..211
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(234..281)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gatctctcgt gggatcattg tttttctctt gattcccact ttgtggttct aagtactgtg
       61 gtttccaaat gtgtcagttt catagcctga agaacgagat cagcagcctc tgttccacat
      121 acacttcatt ctcagtattg ttttgccaag ttctaattcc atcagaagct ggtcgagata
      181 acttcgtata atgtatgcta tacgaagtta taggatccgg ccgatcgaat tctgaagttc
      241 ctatactttc tagagaatag gaacttcgga ataggaactt caaagaattc ctgcagcccg
      301 ggaccccacg cccctcttta atacgacggg caatttgcac ttcagaaaat gaagagtttg
      361 ctttagccat aacaaaagtc cagtatgctt tttcacagca taactggact gatttcagtt
      421 tacaactatt ctgtctagtt taagacttta ttgtcatagt ttagatctat tttgttcagt
      481 ttaagacttt attgtccgcc cacatacgga tggcatgaca gtaagagctc tgcctgttca
      541 tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg
      601 gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtg
//
