LOCUS       sequence-33488-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Aoyama M, Agari K, Sun-Wada GH, Futai M, Wada Y
  TITLE     Simple and straightforward construction of a mouse gene targeting 
            vector using in vitro transposition reactions.
  JOURNAL   Nucleic Acids Res. 2005  . 33(5):e52.
  PUBMED    15784610
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2005  . 33(5):e52."
COMMENT     Sequence Label: pGPS21loxFRTNeo
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        20..67
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      81..90
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(150..169)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     760..779
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tctccgggcc tttcgacctg ttgacaatta atcatcggca tagtatatcg gcatagtata
       61 atacgactca ctataggagg gccaccatgg gatcggccat tgaacaagat ggattgcacg
      121 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
      181 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
      241 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
      301 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
      361 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
      421 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
      481 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
      541 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
      601 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
      661 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
      721 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
      781 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
      841 ccgattcgca gcgcatcgct c
//
