LOCUS       sequence-334885-        3968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible gene expression, pCDF origin, CymRAM repressor, PCymRC 
            promoter, mRFP, Carbenicillin Resistance Marker. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3968)
  TITLE     Reisch Lab Duet Plasmids
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDCyR4
FEATURES             Location/Qualifiers
     source          1..3968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(56..794)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     primer_bind     993..1010
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     994..1010
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1813..1840
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1871..1898
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     misc_RNA        1902..1951
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             1996..2007
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2014..2691
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     promoter        2926..2997
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2998..3066,3067..3858)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3216..3235)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggtggcactt ttcggggaag atagcccagt agctccgggc gtgagactgc ggcgggcgct
       61 gcggacacat acaaagttac ccacagattc cgtggataag caggggacta acatgtgagg
      121 caaaacagca gggccgcgcc ggtggcgttt ttccataggc tccgccctcc tgccagagtt
      181 cacataaaca gacgcttttc cggtgcatct gtgggagccg tgaggctcaa ccatgaatct
      241 gacagtacgg gcgaaacccg acaggactta aagatcccca ccgtttccgg cgggtcgctc
      301 cctcttgcgc tctcctgttc cgaccctgcc gtttaccgga tacctgttcc gcctttctcc
      361 cttacgggaa gtgtggcgct ttctcatagc tcacacactg gtatctcggc tcggtgtagg
      421 tcgttcgctc caagctgggc tgtaagcaag aactccccgt tcagcccgac tgctgcgcct
      481 tatccggtaa ctgttcactt gagtccaacc cggaaaagca cggtaaaacg ccactggcag
      541 cagccattgg taactgggag ttcgcagagg atttgtttag ctaaacacgc ggttgctctt
      601 gaagtgtgcg ccaaagtccg gctacactgg aaggacagat ttggttgctg tgctctgcga
      661 aagccagtta ccacggttaa gcagttcccc aactgactta accttcgatc aaaccacctc
      721 cccaggtggt tttttcgttt acagggcaaa agattacgcg cagaaaaaaa ggatctcaag
      781 aagatccttt gatcttttct actgaaccgc tctagatttc agtgcaattt atctcttcaa
      841 atgtagcacc tgaagtcagc cccatacgat ataagttgta attctcatgt tagtcatgcc
      901 ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc
      961 cggtgcctaa tgagtgagct aagtcacgac gttgtaaaac gacggccagt gatcaacgtt
     1021 tgaattttgc ataacgttca cgtgcaattt ccagggtgct attacgcaca cgttcaaaac
     1081 gttctttatc tttctgccac aggctacgaa ctaccagacc acgaacgctg ttaaaaatca
     1141 gccacagaat atcttcggca tcatcacggc tcagaccacg gctcaccagc acacccagcc
     1201 acatatcttc aacaacaaaa cgattacgtt caacggtacg ctgaatacct tcacgcagtg
     1261 ccggatcacg atctgctgca acaatcagat ccaggccgat gctaaaatca tcatccagaa
     1321 aaaattctgc tgcatcatcc agcatctgct gaataacatc atcttccggt ttcagttttg
     1381 ccagacgtgc acggctacgt tcggtaatct gctcatacag ccattcaaag gttgccagca
     1441 gcagttccag tttggtcgga aaatgatggc tctgtgcacc acggctaaca ccggctgcac
     1501 ccggaacatc tgcaatacga aaacctgcat aacctttttc acgcagaaca cccagtgctg
     1561 ctgcaatcag tttaccctgg gtttccattg cacgttctgc ctgggtacga cgtttcgggc
     1621 tcataagaat ctccgtttgg tatttactat gaatattcca ttgggcgcta tcatgccata
     1681 ccgcgaaagg ttttgcgcca ttcgatggcg cgccgcccca attattgaag gcctccctaa
     1741 cggggggcct ttttttgttt ctggtctccc gcttaacgat cgttggctgg gatacatatg
     1801 tcaatgtacc ggaacaaaca gacaatctgg tctgtttgta ttatggaaaa tttttctgta
     1861 taatagattc aacaaacaga caatctggtc tgtttgtaaa gctgtcaccg gatgtgcttt
     1921 ccggtctgat gagtccgtga ggacgaaaca gcctctacaa ataattttgt ttaagaactg
     1981 ggaagagcgc agattaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa
     2041 gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc
     2101 gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc
     2161 aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc
     2221 aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa
     2281 ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag
     2341 gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc
     2401 ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt
     2461 atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac
     2521 ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg
     2581 ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc
     2641 atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga
     2701 tagtgctagt gtagatcgcg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     2761 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgcatgcata aaaactgttg
     2821 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
     2881 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccattc aaatatgtat
     2941 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     3001 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     3061 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     3121 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     3181 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     3241 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     3301 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     3361 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     3421 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3481 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3541 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3601 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3661 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3721 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3781 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3841 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3901 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3961 aaaatcca
//
