LOCUS       sequence-33491-e         550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Aoyama M, Agari K, Sun-Wada GH, Futai M, Wada Y
  TITLE     Simple and straightforward construction of a mouse gene targeting 
            vector using in vitro transposition reactions.
  JOURNAL   Nucleic Acids Res. 2005  . 33(5):e52.
  PUBMED    15784610
REFERENCE   2  (bases 1 to 550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2005  . 33(5):e52."
COMMENT     Sequence Label: pMODloxZeo3DAmp
FEATURES             Location/Qualifiers
     source          1..550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    358..479
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(395..414)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     449..468
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    490..523
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
       61 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
      121 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
      181 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
      241 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgaccgacg
      301 ccgaccaaca ccgccggtcc gacgcggccc gacgggtccg aggggggtcg acctcgaaac
      361 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
      421 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgcatcttat
      481 catgtctgga taacttcgta taatgtatgc tatacgaagt tatgatcgat gatggttgag
      541 atgtgtataa
//
