LOCUS       sequence-334919-       15506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V 
            mutant signaling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15506)
  AUTHORS   Yu Y, Alvarado R, Petty LE, Bohlender RJ, Shaw DM, Below JE, Bejar 
            N, Ruiz OE, Tandon B, Eisenhoffer GT, Kiss DL, Huff CD, Letra A, 
            Hecht JT
  TITLE     Polygenic risk impacts PDGFRA mutation penetrance in non-syndromic 
            cleft lip and palate.
  JOURNAL   Hum Mol Genet. 2022 Jul 21;31(14):2348-2357. doi: 
            10.1093/hmg/ddac037.
  PUBMED    35147171
REFERENCE   2  (bases 1 to 15506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Hum Mol Genet"; date: "2022-07-21- 21"; volume: "31"; issue: "14"; 
            pages: "2348-2357"
COMMENT     Sequence Label: pCEP4-myc-PDGFRA-D842V_EGFP-PEST_reporter
FEATURES             Location/Qualifiers
     source          1..15506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    14..148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(290..312)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      898..2687
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(2993..4918)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(5353..5371)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5439..5543
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5544..5612,5613..6404)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5762..5781)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6575..7163
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7064..7083
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7925..7973
                     /note="synthetic polyadenylation signal"
     misc_feature    7987..8078
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     8027..8046
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        8257..8288
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             join(8329..8331,8332..8334,8335..9045)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8329..8331,8332..8334,8335..9045)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8374..8395)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8635..8654)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8982..9003
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    9232..9353
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        9581..9726
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9766..10791
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9766..10791
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(10812..10831)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10856..10904
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11557..11936
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11937..12140
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12091..12111
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12137..12161
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             12243..12272
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gccggcaagg ccggatccag acatgataag atacattgat gagtttggac aaaccacaac
       61 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      121 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
      181 ggttcagggg gaggtgggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
      241 gctgattatg atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
      301 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
      361 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc ggtcgactct
      421 agagccccgc cgccggacga actaaacctg actacggcat ctctgcccct tcttcgctgg
      481 tacgaggagc gcttttgttt tgtattcggg gcagtgcatg taatcccttc agttggttgg
      541 tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca aacacaaagg
      601 ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc aacactgagt
      661 ggctttcatc ctggagcaga ctttgcatgc tgtggactgc aacacaacat tgcctttatg
      721 tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc ccaggggccc
      781 aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag ctaccgataa
      841 gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt aaccctaaac
      901 gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct aattcaatag
      961 catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa gggtcctaag
     1021 gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg ggtcaggatt
     1081 ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca aggagcgggc
     1141 agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc taatagaata
     1201 actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga
     1261 cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta tgacaccaat
     1321 ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac attctgaata
     1381 tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat gtccatctca
     1441 cacgaattta tggctatggg caacacataa tcctagtgca atatgatact ggggttatta
     1501 agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc tatttgtaac
     1561 aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc taacaccccc
     1621 gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt ggccactctt
     1681 ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc tgcggttttg
     1741 gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc actgcggtca
     1801 aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata agtaggtggg
     1861 cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca cacttgcgcc
     1921 tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag agcacggtgg
     1981 gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata tgctatccta
     2041 atctatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     2101 atctatatct gggtagtata tgctatccta atttatatct gggtagcata ggctatccta
     2161 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     2221 atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata tgctatccta
     2281 atttatatct gggtagcata tactacccaa atatctggat agcatatgct atcctaatct
     2341 atatctgggt agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     2401 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     2461 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     2521 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctcatgc
     2581 atatacagtc agcatatgat acccagtagt agagtgggag tgctatcctt tgcatatgcc
     2641 gccacctccc aagggggcgt gaattttcgc tgcttgtcct tttcctgcat gctggttgct
     2701 cccattctta ggtgaattta aggaggccag gctaaagccg tcgcatgtct gattgctcac
     2761 caggtaaatg tcgctaatgt tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac
     2821 gtgacaacat gggtatgccc aattgcccca tgttgggagg acgaaaatgg tgacaagaca
     2881 gatggccaga aatacaccaa cagcacgcat gatgtctact ggggatttat tctttagtgc
     2941 gggggaatac acggctttta atacgattga gggcgtctcc taacaagtta catcactcct
     3001 gcccttcctc accctcatct ccatcacctc cttcatctcc gtcatctccg tcatcaccct
     3061 ccgcggcagc cccttccacc ataggtggaa accagggagg caaatctact ccatcgtcaa
     3121 agctgcacac agtcaccctg atattgcagg taggagcggg ctttgtcata acaaggtcct
     3181 taatcgcatc cttcaaaacc tcagcaaata tatgagtttg taaaaagacc atgaaataac
     3241 agacaatgga ctcccttagc gggccaggtt gtgggccggg tccaggggcc attccaaagg
     3301 ggagacgact caatggtgta agacgacatt gtggaatagc aagggcagtt cctcgcctta
     3361 ggttgtaaag ggaggtctta ctacctccat atacgaacac accggcgacc caagttcctt
     3421 cgtcggtagt cctttctacg tgactcctag ccaggagagc tcttaaacct tctgcaatgt
     3481 tctcaaattt cgggttggaa cctccttgac cacgatgctt tccaaaccac cctccttttt
     3541 tgcgcctgcc tccatcaccc tgaccccggg gtccagtgct tgggccttct cctgggtcat
     3601 ctgcggggcc ctgctctatc gctcccgggg gcacgtcagg ctcaccatct gggccacctt
     3661 cttggtggta ttcaaaataa tcggcttccc ctacagggtg gaaaaatggc cttctacctg
     3721 gagggggcct gcgcggtgga gacccggatg atgatgactg actactggga ctcctgggcc
     3781 tcttttctcc acgtccacga cctctccccc tggctctttc acgacttccc cccctggctc
     3841 tttcacgtcc tctaccccgg cggcctccac tacctcctcg accccggcct ccactacctc
     3901 ctcgaccccg gcctccactg cctcctcgac cccggcctcc acctcctgct cctgcccctc
     3961 ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg
     4021 cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc cctcctcctg
     4081 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gctcctgccc
     4141 ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgctcctg
     4201 cccctcctgc tcctgcccct cctgctcctg cccctcctgc ccctcctgcc cctcctcctg
     4261 ctcctgcccc tcctgctcct gcccctcctg cccctcctgc ccctcctgct cctgcccctc
     4321 ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc
     4381 ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc
     4441 ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct cctgcccctc
     4501 ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc
     4561 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
     4621 ctcctgcccc tcccgctcct gctcctgctc ctgttccacc gtgggtccct ttgcagccaa
     4681 tgcaacttgg acgtttttgg ggtctccgga caccatctct atgtcttggc cctgatcctg
     4741 agccgcccgg ggctcctggt cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc
     4801 catggttatc accccctctt ctttgaggtc cactgccgcc ggagccttct ggtccagatg
     4861 tgtctccctt ctctcctagg ccatttccag gtcctgtacc tggcccctcg tcagacatga
     4921 ttcacactaa aagagatcaa tagacatctt tattagacga cgctcagtga atacagggag
     4981 tgcagactcc tgccccctcc aacagccccc ccaccctcat ccccttcatg gtcgctgtca
     5041 gacagatcca ggtctgaaaa ttccccatcc tccgaaccat cctcgtcctc atcaccaatt
     5101 actcgcagcc cggaaaactc ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc
     5161 caggcctcaa attcctcgtc cccctttttg ctggacggta gggatgggga ttctcgggac
     5221 ccctcctctt cctcttcaag gtcaccagac agagatgcta ctggggcaac ggaagaaaag
     5281 ctgggtgcgg cctgtgagga tcagcttatc gatgataagc tgtcaaacat gagaattctt
     5341 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     5401 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5461 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5521 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5581 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5641 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5701 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5761 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
     5821 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5881 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5941 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6001 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6061 acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa
     6121 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6181 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6241 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6301 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6361 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6421 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6481 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6541 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6601 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6661 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6721 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6781 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6841 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6901 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6961 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7021 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7081 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7141 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7201 tttgctgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg
     7261 gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt
     7321 gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca
     7381 ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac
     7441 ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc
     7501 gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct
     7561 acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga
     7621 atcataatgg ggaaggccat ccagcctcgc gtcgcaagga tcttgccgct attgagatcc
     7681 agttcgatat agcccactct tgcacccagt tgatcttcag catcttttac tttcaccagc
     7741 gtttcggggt gtgcaaaaac aggcaagcaa aatgccgcaa agaagggaat gagtgcgaca
     7801 cgaaaatgtt ggatgctcat actcgtcctt tttcaatatt attgaagcat ttatcagggt
     7861 tactagtacg tctctcaagg ataagtaagt aatattaagg tacgggaggt attggacagg
     7921 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat
     7981 agtactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc
     8041 tgtccccagt gcaagtgcag gtgccagaac atttctctgg cctaactggc cggtacctga
     8101 gctcactagc aggatgtcca tattaggaca tctaggatgt ccatattagg acatctagga
     8161 tgtccatatt aggacatcta ggatgtccat attaggacat ctaggatgtc catattagga
     8221 catctagatc tggcctcggc ggccaagctt agacactaga gggtatataa tggaagctcg
     8281 acttccagct tggcaatccg gtactgttgg taaagccacc ggcgcgccat ggtgagcaag
     8341 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     8401 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     8461 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     8521 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     8581 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     8641 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     8701 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     8761 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     8821 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     8881 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     8941 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     9001 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagagtca tggttttccc
     9061 ccggaagtcg aagagcagga cgacgggacg ctgccaatgt catgcgcaca agagtctggt
     9121 atggatcgcc acccggctgc gtgtgccagc gcgagaatca atgtataatg aggcgcgccc
     9181 taaggccgcg actctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca
     9241 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     9301 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     9361 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     9421 agtaaaacct ctacaaatgt ggtaaaatcg ataagcgaac gccagcaaga cgtagcccag
     9481 cgcgtcggcc gccatgccct gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt
     9541 gtccccggaa gaaatatatt tgcatgtctt tagttctatg atgacacaaa ccccgcccag
     9601 cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc
     9661 cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc
     9721 gcttaacagc gtcaacagcg tgccgcagat cccgggcaat gagatatgaa aaagcctgaa
     9781 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
     9841 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     9901 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     9961 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag
    10021 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
    10081 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
    10141 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
    10201 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
    10261 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
    10321 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
    10381 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
    10441 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
    10501 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
    10561 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
    10621 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
    10681 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
    10741 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata ggggagatgg
    10801 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
    10861 ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg
    10921 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
    10981 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
    11041 caacgtcggg gcggcaggcc ctgccatagc cactggcccc gtgggttagg gacggggtcc
    11101 cccatgggga atggtttatg gttcgtgggg gttattattt tgggcgttgc gtggggtctg
    11161 gtccacgact ggactgagca gacagaccca tggtttttgg atggcctggg catggaccgc
    11221 atgtactggc gcgacacgaa caccgggcgt ctgtggctgc caaacacccc cgacccccaa
    11281 aaaccaccgc gcggatttct ggcgtgccaa gctagtcgac caattctcat gtttgacagc
    11341 ttatcatcgc agatccgggc aacgttgttg cattgctgca ggcgcagaac tggtaggtat
    11401 ggaagatcta tacattgaat caatattggc aattagccat attagtcatt ggttatatag
    11461 cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca
    11521 tttatattgg ctcatgtcca atatgaccgc catgttgaca ttgattattg actagttatt
    11581 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
    11641 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
    11701 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
    11761 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc
    11821 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
    11881 tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
    11941 tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
    12001 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
    12061 caaaatgtcg taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg
    12121 aggtctatat aagcagagct cgtttagtga accgtcagat ctctagaagc tgggtaccag
    12181 ctgctagcgc caccatgaag accatcatcg ccctgagcta catcttctgc ctggtgttcg
    12241 ccgagcagaa attgatcagc gaggaggacc tgggcagccc tggaggcgcc tctggccagc
    12301 tttcattacc ctctatcctt ccaaatgaaa atgaaaaggt tgtgcagctg aattcatcct
    12361 tttctctgag atgctttggg gagagtgaag tgagctggca gtaccccatg tctgaagaag
    12421 agagctccga tgtggaaatc agaaatgaag aaaacaacag cggccttttt gtgacggtct
    12481 tggaagtgag cagtgcctcg gcggcccaca cagggttgta cacttgctat tacaaccaca
    12541 ctcagacaga agagaatgag cttgaaggca ggcacattta catctatgtg ccagacccag
    12601 atgtagcctt tgtacctcta ggaatgacgg attatttagt catcgtggag gatgatgatt
    12661 ctgccattat accttgtcgc acaactgatc ccgagactcc tgtaacctta cacaacagtg
    12721 agggggtggt acctgcctcc tatgacagca gacagggctt taatgggacc ttcactgtag
    12781 ggccctatat ctgtgaggcc accgtcaaag gaaagaagtt ccagaccatc ccatttaatg
    12841 tttatgcttt aaaagcaaca tcagagctgg atctagaaat ggaagctctt aaaaccgtgt
    12901 ataagtcagg ggaaacgatt gtggtcacct gtgctgtttt taacaatgag gtggttgacc
    12961 ttcaatggac ttaccctgga gaagtgaaag gcaaaggcat cacaatgctg gaagaaatca
    13021 aagtcccatc catcaaattg gtgtacactt tgacggtccc cgaggccacg gtgaaagaca
    13081 gtggagatta cgaatgtgct gcccgccagg ctaccaggga ggtcaaagaa atgaagaaag
    13141 tcactatttc tgtccatgag aaaggtttca ttgaaatcaa acccaccttc agccagttgg
    13201 aagctgtcaa cctgcatgaa gtcaaacatt ttgttgtaga ggtgcgggcc tacccacctc
    13261 ccaggatatc ctggctgaaa aacaatctga ctctgattga aaatctcact gagatcacca
    13321 ctgatgtgga aaagattcag gaaataaggt atcgaagcaa attaaagctg atccgtgcta
    13381 aggaagaaga cagtggccat tatactattg tagctcaaaa tgaagatgct gtgaagagct
    13441 atacttttga actgttaact caagttcctt catccattct ggacttggtc gatgatcacc
    13501 atggctcaac tgggggacag accgtacggt gcacagctga aggcacgccg cttcctgata
    13561 ttgagtggat gatatgcaaa gatattaaga aatgtaataa tgaaacttcc tggactattt
    13621 tggccaacaa tgtctcaaac atcatcacgg agatccactc ccgagacagg agtaccgtgg
    13681 agggccgtgt gactttcgcc aaagtggagg agaccatcgc cgtgcgatgc ctggctaaga
    13741 atctccttgg agctgagaac cgagagctga agctggtggc tcccaccctg cgttctgaac
    13801 tcacggtggc tgctgcagtc ctggtgctgt tggtgattgt gatcatctca cttattgtcc
    13861 tggttgtcat ttggaaacag aaaccgaggt atgaaattcg ctggagggtc attgaatcaa
    13921 tcagcccgga tggacatgaa tatatttatg tggacccgat gcagctgcct tatgactcaa
    13981 gatgggagtt tccaagagat ggactagtgc ttggtcgggt cttggggtct ggagcgtttg
    14041 ggaaggtggt tgaaggaaca gcctatggat taagccggtc ccaacctgtc atgaaagttg
    14101 cagtgaagat gctaaaaccc acggccagat ccagtgaaaa acaagctctc atgtctgaac
    14161 tgaagataat gactcacctg gggccacatt tgaacattgt aaacttgctg ggagcctgca
    14221 ccaagtcagg ccccatttac atcatcacag agtattgctt ctatggagat ttggtcaact
    14281 atttgcataa gaatagggat agcttcctga gccaccaccc agagaagcca aagaaagagc
    14341 tggatatctt tggattgaac cctgctgatg aaagcacacg gagctatgtt attttatctt
    14401 ttgaaaacaa tggtgactac atggacatga agcaggctga tactacacag tatgtcccca
    14461 tgctagaaag gaaagaggtt tctaaatatt ccgacatcca gagatcactc tatgatcgtc
    14521 cagcctcata taagaagaaa tctatgttag actcagaagt caaaaacctc ctttcagatg
    14581 ataactcaga aggccttact ttattggatt tgttgagctt cacctatcaa gttgcccgag
    14641 gaatggagtt tttggcttca aaaaattgtg tccaccgtga tctggctgct cgcaacgtcc
    14701 tcctggcaca aggaaaaatt gtgaagatct gtgactttgg cctggccaga gtcatcatgc
    14761 atgattcgaa ctatgtgtcg aaaggcagta cctttctgcc cgtgaagtgg atggctcctg
    14821 agagcatctt tgacaacctc tacaccacac tgagtgatgt ctggtcttat ggcattctgc
    14881 tctgggagat cttttccctt ggtggcaccc cttaccccgg catgatggtg gattctactt
    14941 tctacaataa gatcaagagt gggtaccgga tggccaagcc tgaccacgct accagtgaag
    15001 tctacgagat catggtgaaa tgctggaaca gtgagccgga gaagagaccc tccttttacc
    15061 acctgagtga gattgtggag aatctgctgc ctggacaata taaaaagagt tatgaaaaaa
    15121 ttcacctgga cttcctgaag agtgaccatc ctgctgtggc acgcatgcgt gtggactcag
    15181 acaatgcata cattggtgtc acctacaaaa acgaggaaga caagctgaag gactgggagg
    15241 gtggtctgga tgagcagaga ctgagcgctg acagtggcta catcattcct ctgcctgaca
    15301 ttgaccctgt ccctgaggag gaggacctgg gcaagaggaa cagacacagc tcgcagacct
    15361 ctgaagagag tgccattgag acgggttcca gcagttccac cttcatcaag agagaggacg
    15421 agaccattga agacatcgac atgatggacg acatcggcat agactcttca gacctggtgg
    15481 aagacagctt cctgtaatga ctcgag
//
