LOCUS       sequence-33492-e         534 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Aoyama M, Agari K, Sun-Wada GH, Futai M, Wada Y
  TITLE     Simple and straightforward construction of a mouse gene targeting 
            vector using in vitro transposition reactions.
  JOURNAL   Nucleic Acids Res. 2005  . 33(5):e52.
  PUBMED    15784610
REFERENCE   2  (bases 1 to 534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2005  . 33(5):e52."
COMMENT     Sequence Label: pMODloxZeo3DAmp
FEATURES             Location/Qualifiers
     source          1..534
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(140..159)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     259..281
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(319..337)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
       61 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
      121 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
      181 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
      241 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
      301 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      361 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      421 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      481 tgcttcaata atactcacca gtcacagaaa agcatcttac ggatggcatg acag
//
