LOCUS       sequence-33493-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Aoyama M, Agari K, Sun-Wada GH, Futai M, Wada Y
  TITLE     Simple and straightforward construction of a mouse gene targeting 
            vector using in vitro transposition reactions.
  JOURNAL   Nucleic Acids Res. 2005  . 33(5):e52.
  PUBMED    15784610
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2005  . 33(5):e52."
COMMENT     Sequence Label: pBSDT-AII
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          146..211
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(288..860)
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
ORIGIN
        1 acgactcact atagggcgaa ttggagctgt ttaaacggac cgcggtggcg gccgctctag
       61 gatccggccg gcccgggcga tcccccgact ctagtggatc tgcattccac cactgctccc
      121 attcatcagt tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa
      181 cacattatac acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt
      241 tgtccaatta tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga
      301 tttcctgcac aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg
      361 gtttcaaaat taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata
      421 tattcaacgc tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca
      481 tcaccgaacc ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga
      541 cttaaaccta actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc
      601 gtcagtcctg gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca
      661 ttatctacag agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa
      721 tcatcgtcat aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa
      781 tctacataac caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta
      841 gaagaatcaa caacatcatc agggtccatg gcgaggacgt gcag
//
