LOCUS       sequence-334936-        6057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct 
            expression in mammalian cells. pAAV vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Kaberniuk AA, Baloban M, Monakhov MV, Shcherbakova DM, Verkhusha VV
  TITLE     Single-component near-infrared optogenetic systems for gene 
            transcription regulation.
  JOURNAL   Nat Commun. 2021 Jun 23;12(1):3859. doi: 10.1038/s41467-021-24212-7.
  PUBMED    34162879
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-23"; volume: "12(1):3859. doi"; pages: " 
            10.1038/s41467-021-24212-7"
COMMENT     Sequence Label: pAAV-CaMKII-GAL4-iLight-VP16
FEATURES             Location/Qualifiers
     source          1..6057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      552..561
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2697..2930
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(2989..3009)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     3191..3210
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3320..3460
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3320..3449
                     /label=AAV2 ITR
     rep_origin      3535..3990
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3622..3641)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3832..3853
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4007..4026)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4126..4148
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4186..4204)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4272..4376
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4377..4445,4446..5237)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4595..4614)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5408..5996
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5897..5916
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcag atgctgcaca cttgtggact aagtttgttc gcatcccctt
      181 ctccaacccc ctcagtacat caccctgggg gaacagggtc cacttgctcc tgggcccaca
      241 cagtcctgca gtattgtgta tataaggcca gggcaaagag gagcaggttt taaagtgaaa
      301 ggcaggcagg tgttggggag gcagttaccg gggcaacggg aacagggcgt ttcggaggtg
      361 gttgccatgg ggacctggat gctgacgaag gctcgcgagg ctgtgagcag ccacagtgcc
      421 ctgctcagaa gccccaagct cgtcagtcaa gccggttctc cgtttgcact caggagcacg
      481 ggcaggcgag tggcccctag ttctgggggc agcgtgaaaa gacggtatcg ataagctaga
      541 ttcgagctag cgccaccatg gcgcgcaaga agaagaggaa gtccggaggt ggtggaaagc
      601 tactgtcttc tatcgaacaa gcatgcgata tttgccgact taaaaagctc aagtgctcca
      661 aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg ggagtgtcgc tactctccca
      721 aaaccaaaag gtctccgctg actagggcac atctgacaga agtggaatca aggctagaaa
      781 gactggaaca gactagtgct ggtgggtccg ccggcgggag tgctggcggt agtgccggcg
      841 gtgaattcgc cgccgacctg ggctctgacg atatcagcaa gctgatcgcc gcctgcgatc
      901 aggagccaat ccacatcccc aatgccatcc agccatttgg cgccatgctg atcgtggaga
      961 aggacacaca gcagatcgtg tacgcctctg ccaacagcgc cgagtacttc agcgtggccg
     1021 acaataccat ccacgagctg tccgatttca agcaggccaa catcaattct ctgctgcccg
     1081 agcagctgat cagcggcctg acatccgcca tctctgagaa cgagcctatc tgggtggaga
     1141 ccgacaggct gagctttctg ggctggcgcc acgagaacta ctatatcatc gaggtggaga
     1201 gataccacgt gcagacatcc aattggttcg agatccagtt tcagcgggcc ttccagaagc
     1261 tgagaaactg taagacccac aacgatctga tcaataccct gacacggctg atccaggaga
     1321 tcagcggcta cgacagagtg atgatctatc agttcgatcc cgagtggaat ggcagagtga
     1381 tcgccgagag cgtgagacag ctgtttacct ccatgctgaa ccaccacttc ccagcctctg
     1441 acatccctgc acaggccagg gccatgtaca gcatcaaccc aatccgcatc atccccgatg
     1501 tgaatgccga gccccagcct ctgcacatga tccacaagcc acagaacaca gaggccgtga
     1561 atctgtcctg cggcgtgctg agggccgtgt ctccactgca catgcagtat ctgcgcaact
     1621 ttggcgtgtc tgccagcacc tccatcggca tcttcaatga ggacaagctg tggggcatcg
     1681 tggcctgtca ccacacaaag cctagggcca tcggccggag aatcaggcac ctgctggtgc
     1741 gcaccgtgga gtttgcagca gagcgcctgt ggctgatcca ctccaggaat gtggagcggt
     1801 acatggtgac agtgcaggca gcccgggagc agctgtctac cacagccgac gataagcaca
     1861 gctcccacga gatcgtgatc gagcacgccg ccgactggtg caagctgttc cggtgtgatg
     1921 gcgtgggcta cctgagaggc gaggagctga ccacatatgg cgagacccct gatcagacca
     1981 caatcaacaa gctggtggag tggctggagg agaatggcaa gaagagcctg ttttggcact
     2041 cccacatgct gaaggaggac gcacctggac tgctgccaga tggcagccgg ttcgcaggac
     2101 tgctggccat cccactgaag tctgacgccg atctgtttag ctacctgctg ctgttcaggg
     2161 tggcacagaa cgaggtgcgc acatgggcag gcaagcctga gaagctgtcc gtggagacct
     2221 ctacaggcac catggtgggc ccacggaagt cttttgaggc ctggcaggac gaggtgagcg
     2281 gcaagtccca gccttggaga accgcacagc tgtatgcagc ccgggacatc gcccgggacc
     2341 tgctgatcgt ggccgatagc atgcagctga acctgctgaa tgaccagctg gccgatgcca
     2401 acgagaatct ggagaagctg gcctccttcg acgatctgac ctctggcggc ggtaccagcg
     2461 ggggtggtgg atcaggtgga ggaggttctg gaggtggtgg atcaggagga ggtggttctg
     2521 gaggtggtgg atcaggagga agatctgcgt acagccgcgc gcgtacgaaa aacaattacg
     2581 ggtctaccat cgagggcctg ctcgatctcc cggacgacga cgcccccgaa gaggcggggc
     2641 tggcggctcc gcgcctgtcc tttctccccg cgggacacac gcgcagactg tcgacggccc
     2701 ccccgaccga tgtcagcctg ggggacgagc tccacttaga cggcgaggac gtggcgatgg
     2761 cgcatgccga cgcgctagac gatttcgatc tggacatgtt gggggacggg gattcccctg
     2821 gtccgggatt taccccccac gactccgccc cctacggcgc tctggatatg gccgacttcg
     2881 agtttgagca gatgtttacc gatgcccttg gaattgacga gtacggtggg taaataatca
     2941 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3001 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3061 tttcattttc tcctccttgt ataaatcctg gttagttctt gccacggcgg aactcatcgc
     3121 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     3181 gtttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     3241 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga
     3301 ggttttttaa agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     3361 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     3421 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
     3481 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     3541 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3601 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3661 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3721 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     3781 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3841 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
     3901 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3961 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     4021 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     4081 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     4141 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     4201 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     4261 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4321 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4381 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4441 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4501 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4561 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     4621 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     4681 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     4741 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     4801 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     4861 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     4921 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     4981 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5041 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5101 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5161 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5221 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5281 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5341 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5401 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5461 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5521 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     5581 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5641 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5701 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5761 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5821 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5881 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5941 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6001 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
