LOCUS       sequence-334943-        5607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of iLight with 6xHis N-terminal tag in bacteria..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Kaberniuk AA, Baloban M, Monakhov MV, Shcherbakova DM, Verkhusha VV
  TITLE     Single-component near-infrared optogenetic systems for gene 
            transcription regulation.
  JOURNAL   Nat Commun. 2021 Jun 23;12(1):3859. doi: 10.1038/s41467-021-24212-7.
  PUBMED    34162879
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-23"; volume: "12(1):3859. doi"; pages: " 
            10.1038/s41467-021-24212-7"
COMMENT     Sequence Label: pBAD/His-D-iLight
FEATURES             Location/Qualifiers
     source          1..5607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             328..345
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2008..2025)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2178..2264
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2356..2383
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2402..2493
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2494..2562,2563..3354)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2712..2731)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3525..4113
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4014..4033
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4267..4284
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4299..4439
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4365..4384)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4525..4547
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4703..5581)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat ggggagccac catcaccatc accatggcag atctgcagct
      361 ggtaccgcag cagatctggg tagtgatgat atcagcaaac tgattgcagc atgtgatcaa
      421 gaaccgattc atattccgaa tgcaattcag ccgtttggtg caatgctgat tgttgaaaaa
      481 gatacccagc agattgttta tgcaagcgca aatagcgcag aatatttcag cgttgcagat
      541 aacaccattc atgaactgag cgattttaaa caggccaaca ttaatagcct gctgccggaa
      601 caactgatta gcggtctgac aagcgcaatt agtgaaaatg aaccgatttg ggttgaaacc
      661 gatcgtctga gctttctggg ttggcgtcat gaaaactatt acatcattga agtggaacgc
      721 tatcatgtgc agaccagcaa ttggtttgaa attcagtttc agcgtgcctt tcagaaactg
      781 cgtaattgca aaacccataa cgatctgatt aataccctga cccgtctgat tcaagaaatc
      841 agcggttatg atcgcgtgat gatctatcaa tttgatccgg aatggaatgg tcgtgttatt
      901 gcagaaagcg ttcgtcagct gtttaccagc atgctgaatc atcattttcc ggcaagcgat
      961 attccggcac aggcacgtgc aatgtatagc attaatccga ttcgtattat cccggatgtt
     1021 aatgcagaac cgcagccgct gcacatgatt cataaaccgc aaaataccga agcagttaat
     1081 ctgagctgcg gtgttctgcg tgcagttagc cctctgcaca tgcagtatct gcgtaatttt
     1141 ggtgttagcg caagcaccag cattggcatt tttaacgaag ataaactgtg gggtatcgtt
     1201 gcatgccatc ataccaaacc gcgtgcaatt ggtcgtcgta ttcgtcatct gctggttcgt
     1261 accgttgaat ttgcagcaga acgtctgtgg ctgattcata gccgtaatgt tgaacgttat
     1321 atggttaccg ttcaggcagc acgtgaacag ctgagcacca ccgcagatga taaacattca
     1381 agccatgaaa tcgtgattga acatgcagca gattggtgta aactgtttcg ttgtgatggt
     1441 gttggttatc tgcgtggaga agaactgacc acctatggtg aaacaccgga tcagaccacc
     1501 attaacaaac tggttgaatg gctggaagag aacggtaaaa aaagcctgtt ttggcatagc
     1561 cacatgctga aagaagatgc accgggtctg ctgccggatg gtagccgttt tgcaggtctg
     1621 ctggcaattc cgctgaaaag tgatgcagac ctgtttagct atctgctgct gtttcgtgtg
     1681 gcacagaatg aagttcgtac ctgggcgggt aaaccggaaa aactgagcgt tgaaaccagc
     1741 actggcacca tggtgggtcc gcgtaaaagt tttgaagcat ggcaggatga agttagcggt
     1801 aaaagccagc cgtggcgtac cgcacagctg tatgcagcac gtgatattgc acgtgatctg
     1861 ctgattgtgg cagatagcat gcagctgaat ctgctgaatg atcagctggc agatgcaaat
     1921 gaaaatctgg aaaaactggc cagctttgat gatctgacct aggaattcga agcttggctg
     1981 ttttggtgga tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg
     2041 tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc
     2101 gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt
     2161 agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2221 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt
     2281 tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca
     2341 ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc
     2401 ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     2461 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     2521 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     2581 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     2641 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     2701 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     2761 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     2821 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     2881 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     2941 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     3001 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     3061 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     3121 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     3181 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     3241 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     3301 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     3361 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     3421 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     3481 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3541 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3601 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3661 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3721 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3781 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3841 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3901 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3961 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4021 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4081 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4141 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4201 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4261 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     4321 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     4381 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     4441 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     4501 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     4561 caccgaaacg cgcgaggcag cagatcaatt cgcgcgcgaa ggcgaagcgg catgcataat
     4621 gtgcctgtca aatggacgaa gcagggattc tgcaaaccct atgctactcc gtcaagccgt
     4681 caattgtctg attcgttacc aattatgaca acttgacggc tacatcattc actttttctt
     4741 cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga
     4801 aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg
     4861 tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa
     4921 tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg
     4981 cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct
     5041 cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca
     5101 gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc
     5161 cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc
     5221 gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc
     5281 gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt
     5341 gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc
     5401 cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc
     5461 ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg
     5521 ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca
     5581 tacttttcat actcccgcca ttcagag
//
