LOCUS       sequence-334944-        5042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Renilla reniformisluciferase (RLuc8) expression in mammlian cells. 
            Reporter plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Kaberniuk AA, Baloban M, Monakhov MV, Shcherbakova DM, Verkhusha VV
  TITLE     Single-component near-infrared optogenetic systems for gene 
            transcription regulation.
  JOURNAL   Nat Commun. 2021 Jun 23;12(1):3859. doi: 10.1038/s41467-021-24212-7.
  PUBMED    34162879
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-23"; volume: "12(1):3859. doi"; pages: " 
            10.1038/s41467-021-24212-7"
COMMENT     Sequence Label: pG12-Rluc8
FEATURES             Location/Qualifiers
     source          1..5042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        15..33
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     15..32
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          1926..1991
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2121..2141
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2566..2700
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2603..2622)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2657..2676
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2710..2729)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2711..2729)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2816..2833)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2987..3575)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3067..3086)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3746..4537,4538..4606))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4369..4388
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4607..4711)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4779..4797
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4835..4857)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4957..4976
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atcatacaca tacgatttag gtgacactat agaactcgag cagctgaagc ttcggagtac
       61 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagt cgactctagc
      121 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagtcga
      181 ctctagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc
      241 gagtcgactc tagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      301 gtcctccggc tagtagcgga gactctagag ggtatataat ggatccccgg gtacccgggg
      361 atccaccatg aaggtgtacg accccgagca gaggaagagg atgatcaccg gcccccagtg
      421 gtgggccagg tgcaagcaga tgaacgtgct ggacagcttc atcaactact acgacagcga
      481 gaagcacgcc gagaacgccg tgatcttcct gcacggcaac gccactagca gctacctgtg
      541 gaggcacgtg gtgccccaca tcgagcccgt ggccaggtgc atcatccccg atctgatcgg
      601 catgggcaag agcggcaaga gcggcaacgg cagctacagg ctgctggacc actacaagta
      661 cctgaccgcc tggttcgagc ttctgaacct gcccaagaag atcatcttcg tgggccacga
      721 ctggggcgcc gccctggcct tccactacgc ctacgagcac caggacagga tcaaggccat
      781 cgtgcacatg gagagcgtgg tggacgtgat cgagagctgg gacgagtggc cagacatcga
      841 ggaggacatc gccctgatca agagcgagga gggcgagaag atggtgctgg agaacaactt
      901 cttcgtggag accgtgctgc ccagcaagat catgagaaag ctggagcccg aggagttcgc
      961 cgcctacctg gagcccttca aggagaaggg cgaggtgaga agacccaccc tgagctggcc
     1021 cagagagatc cccctggtga agggcggcaa gcccgacgtg gtgcagatcg tgagaaacta
     1081 caacgcctac ctgagagcca gcgacgacct gcccaagctg ttcatcgagg gcgaccccgg
     1141 cttcttcagc aacgccatcg tggagggcgc caagaagttc cccaacaccg agttcgtgaa
     1201 ggtgaagggc ctgcacttcc tccaggagga cgcccccgac gagatgggca agtacatcaa
     1261 gagcttcgtg gagagagtgc tgaagaacga gcagtaagcg gccgcgactc tagaattccg
     1321 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
     1381 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
     1441 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     1501 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
     1561 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     1621 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     1681 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     1741 gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggttg
     1801 ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgccgg atctttgtga
     1861 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct
     1921 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
     1981 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta
     2041 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg
     2101 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc
     2161 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct
     2221 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat
     2281 attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta
     2341 ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta
     2401 gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag
     2461 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     2521 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     2581 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     2641 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     2701 tgccggtctc cctatagtga gtcgtattaa tttcgataag ccaggttaac ctgcattaat
     2761 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     2821 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2881 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2941 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3001 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3061 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3121 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3181 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3241 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3301 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3361 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3421 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3481 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3541 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3601 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3661 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3721 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3781 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3841 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3901 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3961 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4021 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4081 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4141 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4201 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4261 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4321 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4381 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4441 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4501 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4561 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4621 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4681 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     4741 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     4801 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     4861 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     4921 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     4981 gagtgcacca tatggacata ttgtcgttag aacgcggcta caattaatac ataaccttat
     5041 gt
//
