LOCUS       sequence-334949-        3507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HSP70B Promoter fused to synthetic 3xP3 eye driver.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: p3xP3-Hsp70b
FEATURES             Location/Qualifiers
     source          1..3507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(174..965,966..1034))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     797..816
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1035..1139)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1217..1672)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1354..1375)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1566..1585
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(1698..1725)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     1866..1883
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1867..1883
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1890..1909
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1890..1908
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1972..2423)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(2003..2024)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     promoter        complement(2544..2562)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2545..2562)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2578..2594)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2578..2594)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      2665..2736
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2751..2768)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2922..3507,1..3))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3002..3021)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
       61 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
      121 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
      181 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
      241 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
      301 gcaatgatac cgcgcgaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
      361 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
      421 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
      481 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
      541 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
      601 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
      661 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
      721 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgacctag ttgctcttgc
      781 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
      841 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
      901 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
      961 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     1021 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     1081 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     1141 acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat gcgtaagtag
     1201 aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     1261 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     1321 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     1381 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcgc tggcccacta
     1441 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     1501 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     1561 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     1621 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgt
     1681 aacattgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc
     1741 tggcagttta tggcgggcgt cctgcccgcc accctttcag ccaaaaaact taagaccgcc
     1801 ggtcttgtcc actaccttgc agtaatgcgg tggacaggat cggcggtttt cttttctctt
     1861 ctcaatgtaa aacgacggcc agtgaattgt aatacgactc actatagggt gaattccgct
     1921 taggacctta gatattgatc tcaaggtctc gcattttgtt ttcctccttt gagagttctc
     1981 ttcttgtatt caataattac ttcttggcag atttcagtag ttgcagttga tttacttggt
     2041 tgctggttac ttttaattga ttcactttaa cttgcacttt actgcagatt gtttagcttg
     2101 ttcagctgcg cttgtttatt tgcttagctt tcgcttagcg acgtgttcac tttgcttgtt
     2161 tgaattgaat tgtcgctccg tagacgaagc gcctctattt atactccggc gctcttttcg
     2221 cgaacattcg aggcgcgctc tctcgaacca acgagagcag tatgccgttt actgtgtgac
     2281 agagtgagag agcattagtg cagagaggga gcgacccaaa aagaaaagag agaataacga
     2341 ataacggcca gagaaatttc tcgagttttc tttctgccaa acaaatgacc taccacaata
     2401 accagtttgt tttgggattc taggggagag ggttcgaaat cgataagctt ggatcctaat
     2461 tgaattagct ctaattgaat tagtctctaa ttgaattaga tccctcccga gaccactcat
     2521 cgcccaatca ctagtgaatt cgttctatag tgtcacctaa ataccataga gccgttggtc
     2581 atagctgttt cctgtcggac caaaacgaaa aaaggccccc ctttcgggag gcctcttttc
     2641 tggaatttgg taccgagcag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     2701 tttcgtttta tctgttgttt gtcggtgaac gctctctgac cttccgcttc ctcgctcact
     2761 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2821 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2881 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2941 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3001 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3061 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3241 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3361 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3481 cagattacgc gcagaaaaaa aggatct
//
