LOCUS       sequence-33495-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Lizier M, Sarra PG, Cauda R, Lucchini F
  TITLE     Comparison of expression vectors in Lactobacillus reuteri strains.
  JOURNAL   FEMS Microbiol Lett. 2010 Jul 1. 308(1):8-15.
  PUBMED    20455948
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol Lett. 2010 Jul 1. 308(1):8-15."
COMMENT     Sequence Label: pBS-ermGFP
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..40
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     25..41
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(624..652)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaagctgggt accgggcccc ccctcgaggt cgacggtatc gataagctta gtctagaatc
       61 gatacgattt tgaagtggca acagataaaa aaaagcagtt taaaattgtt gctgaacttt
      121 taaaacaagc aaatacaatc attgtcgcaa cagatagcga cagagaaggc gaaaacattg
      181 cctggtcgat cattcataaa gcaaatgcct tttctaaaga taaaacgtat aaaagactat
      241 ggatcaatag tttagaaaaa gatgtgatcc gtagcggttt tcaaaatttg caaccaggaa
      301 tgaattacta tcccttttat caagaagcgc acaaaaagaa aaacgaaatg atacaccaat
      361 cagtgcaaaa aaagatataa tgggagataa gacggttcgt gttcgtgctg acttgcacca
      421 tatcataaaa atcgaaacag caaagaatgg cggaaacgta aaagaagtta tggaaataag
      481 acttagaagc aaacttaaga gtgtgttgat agtgcagtat cttaaaattt tgtataatag
      541 gaattgaagt taaattagat gctaaaaatt tgtaattaag aaggagtgaa ttcatgagta
      601 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
      661 atgggtacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
      721 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
      781 ctctcactta tggtgttcaa tgcttttcaa gatatccaga tcatatgaag cggcacgact
      841 tcttcaagag cgccatgcct ga
//
