LOCUS       sequence-334952-       12144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ty5 restrotransposon with a reversed artificial intron used for 
            Calling Cards experiment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12144)
  TITLE     Mitra lab plasmids
REFERENCE   2  (bases 1 to 12144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TY5_hisAI_2micron
FEATURES             Location/Qualifiers
     source          1..12144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        144..331
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     protein_bind    6330..6447
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     promoter        complement(6566..6584)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6605..6621)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6605..6621)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6618..6640)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6629..6645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6653..6659,6660..6677,6678..6683))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6698..6719
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6836..6853)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7007..7595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7087..7106)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7766..8557,8558..8626))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8389..8408
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8627..8731)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    9086..9133
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        10099..10319
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             10320..11123
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YAKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(11254..11709)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11391..11412)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11603..11622
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11835..11857
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11849..11866
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11850..11866
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11873..11892
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11873..11891
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     11924..11940
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11924..11940
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gtactgaaac attacccata tcatgctttt gggttatcac attcaacatc cccccataac
       61 tgaatacgca tgagatttaa ttaattcctg ctgaaccgct cttccgatct ggaagttatc
      121 gagcgcccgg cccttggcct cctctagtac actctatatt tttttatgcc tcggtaatga
      181 ttttcatttt tttttttcca cctagcggat gactcttttt ttttcttagc gattggcatt
      241 atcacataat gaattataca ttatataaag taatgtgatt tcttcgaaga atatactaaa
      301 aaatgagcag gcaagataaa cgaaggcaaa gatgacagag cagaaagccc tagtaaagcg
      361 tattacaaat gaaaccaaga ttcagattgc gatctcttta aagggtggtc ccctagcgat
      421 agagcactcg atcttcccag aaaaagaggc agaagcagta gcagaacagg ccacacaatc
      481 gcaagtgatt aacgtccaca caggtatagg gtttctggac catatgatac atgctctggc
      541 tgttataaat aataccattt gttagtaaaa attcgagctc ggtacccggg gatcctctag
      601 agtcgacctg cagaaaagcc tcctttagtc catattaaca tacccaagca ttccggctgg
      661 tcgctaatcg ttgagtgcat tggtgactta cacatagacg accatcacac cactgaagac
      721 tgcgggattg ctctcggtca agcttttaaa gaggccctag gggccgtgcg tggagtaaaa
      781 aggtttggat caggatttgc gcctttggat gaggcacttt ccagagcggt ggtagatctt
      841 tcgaacaggc cgtacgcagt tgtcgaactt ggtttgcaaa gggagaaagt aggagatctc
      901 tcttgcgaga tgatcccgca ttttcttgaa agctttgcag aggctagcag aattaccctc
      961 cacgttgatt gtctgcgagg caagaatgat catcaccgta gtgagagtgc gttcaaggct
     1021 cttgcggttg ccataagaga agccacctcg cccaatggta ccaacgatgt tccctccacc
     1081 aaaggtgttc ttatgtagtg acaccgatta tttaaagctg cagcatcgat accgtcgatc
     1141 tgttattttg cagtttctgg ttccctcacc atgacagccc tatgtgcttt gaatgattct
     1201 gctggaagga tttttgtaaa tatgtcagca acttgtctct ttgtaataac atgctctatg
     1261 gtaattatgc ctgctgccac tgcacttctt agcttgtgat atctcaaggc aatgtgtttt
     1321 gttctcgtgt gaaatacagg atgtttagat agttttatag caggttcatt atctacgtat
     1381 aatgttgatg agataagtgg ctggcctaag tgttcaaaca agttttgaat ccattctatc
     1441 tccatgacag tttcacttgc agtaatgtat tctgcctcag tagatgatac aggaatcaca
     1501 cccttgagtt tctttgatga ccacgtaact ggagcaccag caagtagagt cacgtacccc
     1561 ccagtagagt gtgggagatc gtgaattgct ccatgagatg catcacaata tacagttagt
     1621 gccaacagag atccagaacg atacttgaga cacatacttc tggtggtata taggtaccgt
     1681 agaactcgtc gagcagactc caaatggatt gcgcgaggtt cgcgaagaaa cctggagagt
     1741 agtgagaccg gataagatat gtcaggacga ccagtatttg cacaaaagag aagctgtcca
     1801 actatgctct gataaggagt gatgtccttt aggtgcgggg aagttgtttc gaaaagaggc
     1861 tttgagtcac agagcggagt ctgtgtaggc ttacatatgt ttatttcgct ttcagatgca
     1921 gctttagcaa tatagtcttg aagtgagaga gtgatgtctc catttgaaaa ttgattaatg
     1981 ttaagaccga ggaatttgtc aactttacct agatccttca ttgagtataa cttcgttagt
     2041 ttctgcttaa ccctgtcata tattttcgga gagggagcag caacaagtaa gtcgtctacg
     2101 tatagggcaa tgtagatggg accatcagat gtggaacgaa agtataagcc atgttcgcct
     2161 tcatgtcgac gaaaaccaat cttttgaaga gtattgttga tatgttcgtt ccatagtaat
     2221 ggggcttgct tgagtccata cataccgccg tatagttccc atacatagtc gggattactt
     2281 tcattaataa atccgggtgg ttgttttacg tataccggct cattcatttt tgagtttaga
     2341 aacgcggtgt caacgtccat ctgatatact attagtttga ggcagctagc aagggccaga
     2401 aataatctaa cagagtcata tcgaatgact ggtgcaaaag tttcttgata gtcaatacca
     2461 gccttttgag tatgtccttg ggcgacaagg cgagctttgt acgtcccgga gtcttttatt
     2521 gtgaataccc agcgcgatcc aagagctctt ctttgctttg gaagagacac caatttgtac
     2581 gtattgtgat ctttgaacgc ggaaagttcc ttgaggcatg cgttgtgcca ttcttgaaag
     2641 tcaggccttc ccattgcttg ggcataggtt ttcgggtttc ctttttttgt gctatggata
     2701 atagcagtta gagccgcgtt aaatgaggta tttggaggcg atgaatccaa cgatggagga
     2761 gattcgattg aattctcttc agtaactagc ctggttacag caggagtgag gtcagtatca
     2821 cccacatctt gtgagtcgct gatatatgtt ccatcgtgaa cggatagata cctctggtca
     2881 ggtatcggca ttgcgcctac tggaaaccaa tcagtaagag aattagcttg attagttctg
     2941 gcggaattgt tcttcgcttc ctgaagagcc atgcttgaaa gtctttcgat ggacacttct
     3001 tcaccagtac tggcaacatg cagcgtgtca atgttgtttg agtctagagt tcgtagcacg
     3061 gttggaagtc tatttgtcct tgctacagta tcatttgtat tggacaccgt ctttctttgc
     3121 ttggcacttc taacttcagg taagttgtca ctcgatatta cgtttggacg tgatatatca
     3181 tatatttcta tttcgcggga tcgcgttggt ttcttattag ttcttttaac acgtctagtt
     3241 ctatttcggc atgtcggaat atattctgaa tcggatgatt ttggaatttc tcgataagtt
     3301 agtgccgctg gaactctttc aggagttttc tccatagaag aattagtttc gtcttcggta
     3361 gacacggttg gtaaggattc aaagttagaa tgttgtatcc ttgttggtgg acatccggag
     3421 tttggtaagt tgtcttggtt acactcaggg ataggttttt ccatttcgat ttccattgaa
     3481 atatcctcat catcatcaga ggaagagatg ttactggtat taggtttatg tgctggttga
     3541 tttacatttt tgggaacagt tattggcaaa tttgccaagt cagatccgtc gttctttgga
     3601 gcagagacgg ttgaccctgt ttcaggatat ttggacaccc ccccaatagc ggaagtggca
     3661 aagtcgtgag agacgtgaac tgcctctgta tcagcaagag gaaacatgtg ttcgtcaaat
     3721 cgaacttgac tgctcacaaa gatgcggcca gtttcgggat gataaatcct gtatcctcga
     3781 tggttagagt catagccaac cataacacca gatatgacag ttggggcaag tttgccgtct
     3841 cgaagactag gttgtaaggt tgcgtatgct gtacatccaa aaattcgaag atgtgcgata
     3901 ccgaaatctt ttgcggggat gttgaaccac cggcagtatg ggatactgtt atttttggac
     3961 actacagggg acctattgat taaatagact gcgcaagaaa cagcttcaga ccacaaggac
     4021 ggaggaacac gccccccgat aaggagacaa cgagtgcgtt cttcgatggt acgatgtgca
     4081 cgttcaacag caccattttg ataactatga tatggaatag ttaactgatg ctctattcct
     4141 ttagatttaa aaaacgcatg aagattttta tttacgaatt ctgtaccatt gtctgtacga
     4201 acagatccga ctttgaatcc accccgggag ctaaagtatt gttcggtttc gttgatccat
     4261 tccatgaatt ttatcggagc gtctgctttg gattttaaat gtattacaga gtagtagcga
     4321 ctataggcat cacggatagt aagaaaatat ttatttgact gaaactcttg gtagcgaaac
     4381 ccaccacaga gatcagcctg taccatttga agaggttggg tatatttttt gagcgagact
     4441 ttaccctttt tgatgattcc ttttgctagt gagcaggtag gacaaatagt gtattcagcc
     4501 gtcttaaatt ttggaaaacg cagagttcta gccaaggaat tgtaaatatc tgctccggga
     4561 tggcccatac gagcatgcca ttttaccggg agagcagtgg gagaaatgtg ttgcgacaac
     4621 attttatctg caacggtatg aaacgctgcg gaagttatca ttttagatga atccaatgtt
     4681 ttagagcaga agtaattttg cgaagagaag gtagttacgg gcgtaagatc atcgaaaatg
     4741 tacagataat tgtttaactt cccagccgta gaaatcacgt catcaggtga tttcacagtg
     4801 acaccctctt ttgtgaacgt aacagaagag ttggatttag cacatagttt ccaaatggat
     4861 attaggttga ctggtaaatc agggacgtag gatacgtcat ttaattgaac agtgccgatg
     4921 tttacagtcc cggagcccat gatgggtatg gaaccgccga ctcctctgac aaagtctttc
     4981 ttagaggatc tagtaaatga tgagaacata gaacggtcgt ggcacatgtg tgaagtacat
     5041 cctgtgtcaa aaatccaatc tgaggatttg gtagctggga cggtcgaaga taacatacag
     5101 agccaggact ttggtgttat ctgtcctgct gtttggcctt gcgtttcata gtcaggaatc
     5161 gcaacactag cagatgaaga tctattcgtc ttgtcattag gatataattt aaagattctg
     5221 gcttctggta ttcttctttt aagcagagaa cagttgctta agcgatgatt agccccacaa
     5281 atcatacatc ccttatttcc tttcttttga tagcttcttc tgtcttgtgc aaccaatgca
     5341 tctgagactg aagataatga attcgatggc gcctttaatt tacagaatct aatgacttgt
     5401 gacagctgaa gagctttaga gtcaccgaat ttctcgtgga atgattcctt taactcgggt
     5461 aagaaggtca ttaatagcaa cgcgatattg gtatcgcgtc ttttcttttt gtcctgttct
     5521 tcgctattgt cttcaagcat tgtgaatagg gttttcaaaa aggaagcgat ttcatctaca
     5581 ggcttggagg atatatttgg taagtcctct ataagagtaa gcctggatcc aatagtgact
     5641 tcattatagt tttcttcgaa gtgcgagaaa atatcataag cagagaccct tccaagtgaa
     5701 tcatcacata tccagttcat ttcacatgca gcttcaccca cgatagtttt cttgtagagc
     5761 tttaccagga aactgttaaa tacggttttg acaaagcttt catcattcgc gctgagattg
     5821 caatcccttt tcattgattg gatatctccg gtatgaacga attcgttcat atattcgtta
     5881 gcatattcag cagcggtctc gataccgtac agccactgtt tataattgtt gggattagat
     5941 agattaatta tggtctctga agcattgctc tcagggctca taacctgttg acgtagtgaa
     6001 ttacgatcta gcttatatgt cataatgttg taagtttatt ggacctttct atatgttatg
     6061 taaatgtaag aattacatag gtactactct tgtacacaga aactatggtt tcttaagtac
     6121 tgtcggatcg gaggtttgct gctctatgtt ttccttgtct aaaacattac tgaaacaatt
     6181 ctgaaacagt actgaaacat tacccatcgg atcgactcat cctatggttg ttaatttgat
     6241 tcgttcattt gagggtttgt ggggccaggt tactgccaat ttctcctctt cataaccata
     6301 aaagctagta ttgtagaatc tttattgttc ggagcagtgc ggcgcgaggc acatctgcgt
     6361 ttcaggaacg cgaccggtga agacgaggac gcacggagga gagtcttcct tcggagggct
     6421 gtcacccgct cggcggcttc taatccgtac ttcaatatag caatgagcag ttaagcgtat
     6481 tactgaaagt tccaaagaga aggttttttt aggctaagat aatggggctc gacctcgagg
     6541 gggggcccgg taccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
     6601 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6661 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6721 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6781 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6841 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6901 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6961 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7021 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7081 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7141 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7201 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7261 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7321 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7381 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7441 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7501 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7561 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7621 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7681 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7741 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7801 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7861 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7921 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7981 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8041 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     8101 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8161 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8221 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8281 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8341 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     8401 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8461 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8521 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8581 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8641 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8701 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg cctgatccaa
     8761 tatcaaagga aatgatagca ttgaaggatg agactaatcc aattgaggag tggcagcata
     8821 tagaacagct aaagggtagt gctgaaggaa gcatacgata ccccgcatgg aatgggataa
     8881 tatcacagga ggtactagac tacctttcat cctacataaa tagacgcata taagtacgca
     8941 tttaagcata aacacgcact atgccgttct tctcatgtat atatatatac aggcaacacg
     9001 cagatatagg tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg
     9061 aagcgctcgt tttcggaaac gctttgaagt tcctattccg aagttcctat tctctagaaa
     9121 gtataggaac ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa
     9181 aaccaaaaac gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa
     9241 acattgctca aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac
     9301 ccatccacct ttcgctcctt gaacttgcat ctaaactcga cctctacatt ttttatgttt
     9361 atctctagta ttactcttta gacaaaaaaa ttgtagtaag aactattcat agagtgaatc
     9421 gaaaacaata cgaaaatgta aacatttcct atacgtagta tatagagaca aaatagaaga
     9481 aaccgttcat aattttctga ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa
     9541 agtatgcgca atccacatcg gtatagaata taatcgggga tgcctttatc ttgaaaaaat
     9601 gcacccgcag cttcgctagt aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg
     9661 gaagagaaaa tagacaccaa agtagccttc ttctaacctt aacggaccta cagtgcaaaa
     9721 agttatcaag agactgcatt atagagcgca caaaggagaa aaaaagtaat ctaagatgct
     9781 ttgttagaaa aatagcgctc tcgggatgca tttttgtaga acaaaaaaga agtatagatt
     9841 ctttgttggt aaaatagcgc tctcgcgttg catttctgtt ctgtaaaaat gcagctcaga
     9901 ttctttgttt gaaaaattag cgctctcgcg ttgcattttt gttttacaaa aatgaagcac
     9961 agattcttcg ttggtaaaat agcgctttcg cgttgcattt ctgttctgta aaaatgcagc
    10021 tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgttct acaaaatgaa
    10081 gcacagatgc ttccgctttt caattcaatt catcattttt tttttattct tttttttgat
    10141 ttcggtttcc ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga
    10201 aggagcacag acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt
    10261 gcccagtatt cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca
    10321 tgtcgaaagc tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc
    10381 tatttaatat catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca
    10441 ccaaggaatt actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac
    10501 atgtggatat cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat
    10561 ccgccaagta caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag
    10621 tcaaattgca gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg
    10681 cacacggtgt ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa
    10741 caaaggaacc tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta
    10801 ctggagaata tgctaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg
    10861 gctttattgc tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga
    10921 cacccggtgt gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg
    10981 atgatgtggt ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg
    11041 gaagggatgc taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga
    11101 gaagatgcgg ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact
    11161 cacaaattag agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg
    11221 cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa
    11281 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
    11341 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
    11401 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
    11461 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
    11521 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
    11581 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
    11641 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
    11701 agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    11761 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
    11821 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga
    11881 ctcactatag ggcgaattgg agctccaccg cggtggcggc cgctctagaa ctagtggatc
    11941 ctgttgacgt agtgaattac gatctagctt atatgtcata atgttgtaag tttattggac
    12001 ctttctatat gttatgtaaa tgtaagaatt acataggtac tactcttgta cacagaaact
    12061 atggtttctt aagtactgtc ggatcggagg tttgctgctc tatgttttcc ttgtctaaaa
    12121 cattactgaa acaattctga aaca
//
