LOCUS       sequence-334957-        8525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of EGFP and sgRNAs targeted to EGFP for 
            measurement of activity of AsCas12a\EnAsCas12a..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8525)
  TITLE     Doench/Root Lab plasmid for measuring activity of 
            AsCas12a\EnAsCas12a
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRDA_221
FEATURES             Location/Qualifiers
     source          1..8525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1713..1961
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1713..1733
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1884..1903
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2097..2214
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2263..2773
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2692..2710
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(2785..2804)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3281..3301
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3421..3486
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             join(3493..3495,3496..3498,3499..4209)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3493..3495,3496..3498,3499..4209)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3799..3818)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4146..4167
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4378..4966
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4431..4451)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4849..4860)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5038..5271
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5349..5470
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5386..5405)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5440..5459
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5510..5645
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5665..5684)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5666..5684)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5694..5711)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5694..5710)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5703..5725)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5852..6307
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5939..5958)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6149..6170
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6333..6437
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6438..6506,6507..7298)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6656..6675)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7469..8057
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7958..7977
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8211..8228
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8345..8366
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8381..8386,8387..8404,8405..8411)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8419..8435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8424..8446
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8443..8459
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8443..8459
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8478..8498
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8480..8498
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccca gagagggcct atttcccatg attccttcat
     1741 atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca
     1801 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     1861 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     1921 atttcttggc tttatatatc ttgtggaaag gacgaaacac cggtaatttc tactcttgta
     1981 gatcgtcgcc gtccagctcg accaggtaat ttctactgtc gtagatctca gggcggactg
     2041 ggtgctcagt tttttgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt
     2101 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     2161 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     2221 tattacaggg acagcagaga tccactttgg cgccggctcg agggggcccg ggttgcgcct
     2281 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     2341 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     2401 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     2461 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     2521 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     2581 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
     2641 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     2701 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     2761 cctctctccc cagggggatc caccatgacc gagtacaagc ccaccgtgcg cctcgccacc
     2821 cgcgacgacg tgcccagggc cgtccgcacc ctcgccgccg ctttcgccga ctaccccgcc
     2881 acccgccaca ccgtcgatcc cgaccgccac atcgagcggg tcaccgagct gcaagaactc
     2941 ttcctcaccc gcgtcgggct cgacatcggc aaggtgtggg tcgctgacga cggcgccgca
     3001 gtggctgtct ggaccacacc cgagagcgtc gaagctgggg cggtgttcgc cgagatcggc
     3061 cctcgcatgg ccgagttgag cggttcccgg ctggccgctc agcaacagat ggaaggcctc
     3121 ctggctccac accggcccaa ggagcccgca tggttcctgg ccaccgtcgg cgtctctccc
     3181 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggctgccgag
     3241 cgcgccgggg tgcccgcctt cctggagacc tccgcacccc gcaacctccc cttctacgag
     3301 cggctcggct tcaccgtcac cgccgacgtg gaggtgcccg aaggaccacg cacctggtgc
     3361 atgacccgca agcccggtgc cctgcaggga tctggcgaag ccagacacaa acagaaaatt
     3421 gtgaaacaga ctttgaattt tgaccttctc aagttggcgg gagacgtgga gtccaaccca
     3481 gggcccgcta gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3541 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3601 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     3661 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     3721 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     3781 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     3841 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     3901 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     3961 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     4021 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4081 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     4141 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4201 ctgtacaagt ccggatcagg cggaagcggc gggagtgggt cacacggctt tcctcccgaa
     4261 gttgaggagc aggatgatgg caccctgccg atgtcctgcg ctcaggaaag tggcatggac
     4321 aggcacccag ccgcgtgtgc tagtgctaga atcaacgtgt aaacgcgtta agtcgacaat
     4381 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     4441 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     4501 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     4561 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     4621 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     4681 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4741 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     4801 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4861 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4921 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     4981 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     5041 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     5101 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     5161 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     5221 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     5281 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     5341 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5401 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5461 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     5521 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5581 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     5641 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     5701 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     5761 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     5821 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     5881 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5941 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6001 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6061 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6121 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6181 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6241 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     6301 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6361 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6421 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     6481 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6541 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     6601 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     6661 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     6721 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6781 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6841 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6901 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6961 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7021 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7081 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7141 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7201 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7261 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     7321 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     7381 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7441 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     7501 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7561 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     7621 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7681 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7741 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7801 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7861 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7921 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7981 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8041 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8101 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8161 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     8221 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     8281 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     8341 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     8401 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     8461 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     8521 cttaa
//
