LOCUS       sequence-33496-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Lizier M, Sarra PG, Cauda R, Lucchini F
  TITLE     Comparison of expression vectors in Lactobacillus reuteri strains.
  JOURNAL   FEMS Microbiol Lett. 2010 Jul 1. 308(1):8-15.
  PUBMED    20455948
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol Lett. 2010 Jul 1. 308(1):8-15."
COMMENT     Sequence Label: pBS-slpGFP
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..40
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     25..41
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(388..416)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaagctgggt accgggcccc ccctcgaggt cgacggtatc gataagcttg atatcgaatt
       61 cgtggtaagt aataggacgt gcttcaggcg tgttgcctgt acgcatgctg attcttcagc
      121 aagactacta cctcatgaga gttatagact catggatctt gctttgaagg gttttgtaca
      181 ttataggctc ctatcacatg ctgaacctat ggcctattac atttttttat atttcaagga
      241 ggaaaagacc acatgaagaa aaatttaaga atcgttagcg ctgctgctgc tgctttactt
      301 gctgttgctc cagttgctgc ttctgctgta tctactgtta gcagatccac agccaccatg
      361 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
      421 gttaatgggt acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      481 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
      541 actactctca cttatggtgt tcaatgcttt tcaagatatc cagatcatat gaagcggcac
      601 gacttcttca agagcgccat gcctgaggga tacgtgcagg agaggaccat cttcttcaag
      661 gacgacggga actacaagac acgtgctgaa gtcaagtttg agggagacac cctcgtcaac
      721 aggatcgagc ttaagggaat cgatttcaag gaggacggaa acatcctcgg ccacaagttg
      781 gaatacaact acaactccca caacgtatac atcatggccg acaagcaaaa gaacggcatc
      841 aaagccaact tcaagacccg ccacaacatc gaagacggcg gcgtgcaact cgctgatcat
      901 tatcaacaaa atactccaat tggcgatg
//
