LOCUS       sequence-334961-       10146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses rat ACE2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Yurkovetskiy L, Wang X, Pascal KE, Tomkins-Tinch C, Nyalile TP, Wang
            Y, Baum A, Diehl WE, Dauphin A, Carbone C, Veinotte K, Egri SB, 
            Schaffner SF, Lemieux JE, Munro JB, Rafique A, Barve A, Sabeti PC, 
            Kyratsous CA, Dudkina NV, Shen K, Luban J
  TITLE     Structural and Functional Analysis of the D614G SARS-CoV-2 Spike 
            Protein Variant.
  JOURNAL   Cell. 2020 Sep 15. pii: S0092-8674(20)31229-0. doi: 
            10.1016/j.cell.2020.09.032.
  PUBMED    32991842
REFERENCE   2  (bases 1 to 10146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Sep 15. pii: S0092-8674(20)31229-0. doi: 
            10.1016/j.cell.2020.09.032."
COMMENT     Sequence Label: pscALPSpuro-RnACE2 (Rat)
FEATURES             Location/Qualifiers
     source          1..10146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        43..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        424..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     577..597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             640..820
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    867..992
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1489..1722
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1906..1950
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2248..2365
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2502..2909
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             5352..5381
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5885..6484
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5885..5904)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6381..6401
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6494..7082
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6547..6567)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6965..6976)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7600..7780
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7851..8439)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7931..7950)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8610..9401,9402..9470))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9233..9252
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9471..9575)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9650..9669)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9843..9863)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(9848..10146,1..31)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10028..10146,1..17)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10090..10109
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 aggctttttt ggaggcctag gcttttgcaa aaagctcgcg ttgacattga ttattgacta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      601 ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag
      661 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      721 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      781 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
      841 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
      901 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
      961 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     1021 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     1081 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     1141 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     1201 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     1261 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     1321 cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     1381 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     1441 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     1501 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     1561 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     1621 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     1681 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     1741 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     1801 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaattaa caattacaca
     1861 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     1921 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     1981 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     2041 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     2101 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     2161 agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg
     2221 cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     2281 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     2341 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     2401 gttaattaac tgcagccccg ataaaataaa agattttatt tagtctccag aaaaaggggg
     2461 gaatgaaaga ccccacctgt aggtttggca agctagctgc agtaacgcca ttttgcaagg
     2521 catggaaaaa taccaaacca agaatagaga agttcagatc aagggcgggt acatgaaaat
     2581 agctaacgtt gggccaaaca ggatatctgc ggtgagcagt ttcggccccg gcccggggcc
     2641 aagaacagat ggtcaccgca gtttcggccc cggcccgagg ccaagaacag atggtcccca
     2701 gatatggccc aaccctcagc agtttcttaa gacccatcag atgtttccag gctcccccaa
     2761 ggacctgaaa tgaccctgcg ccttatttga attaaccaat cagcctgctt ctcgcttctg
     2821 ttcgcgcgct tctgcttccc gagctctata aaagagctca caacccctca ctcggcgcgc
     2881 cagtcctccg acagactgag tcgcccgggg gtctagaagc cgccaccatg tccagttcat
     2941 gttggctctt gttgtccctt gtggccgtgg cgacagctca gtccctgatc gaagagaaag
     3001 ccgagtcctt cttgaacaaa tttaatcagg aagcagagga tcttagctat caatcaagtc
     3061 ttgccagctg gaactacaac actaacatta cggaagaaaa cgcacaaaaa atgaacgagg
     3121 ctgccgccaa gtggtccgcg ttttacgagg agcagtcaaa gatagcgcaa aacttttccc
     3181 tgcaagagat ccagaatgca accatcaaga ggcaattgaa ggccttgcag cagtcaggct
     3241 cctcagcact ctccccggac aagaacaaac aacttaatac tatccttaac acgatgtcaa
     3301 ctatatactc aacggggaag gtctgcaact ctatgaaccc tcaggaatgc tttcttctcg
     3361 aacccggtct cgatgagata atggctacct ctaccgacta caaccgcagg ttgtgggcat
     3421 gggaaggctg gagggcggaa gtggggaaac aactgagacc actttatgaa gaatacgttg
     3481 tacttaaaaa cgaaatggca agagcgaaca actatgagga ctatggtgac tactggcgag
     3541 gggattatga agcagagggc gttgagggtt ataactataa tcgcaatcaa cttatcgaag
     3601 acgttgagaa cacatttaaa gaaataaagc ctttgtatga gcaactgcac gcctacgtca
     3661 ggaccaaact gatggaggta tacccttcat atatcagtcc cactggctgt ctcccagcgc
     3721 atctgttggg cgacatgtgg ggaagatttt ggactaactt gtatcccctt accactccct
     3781 ttctccagaa gcctaacata gatgttacgg acgcaatggt taatcagtct tgggatgcag
     3841 aacggatttt taaagaggca gagaagtttt tcgttagtgt cggtctccca cagatgaccc
     3901 ctgggttttg gacaaacagc atgcttactg aacccggaga cgataggaaa gtagtttgcc
     3961 atccaacagc ctgggatctt ggacatggag acttcagaat taagatgtgt accaaggtaa
     4021 ctatggacaa ttttctgacg gcgcatcatg agatggggca tatccaatac gatatggcat
     4081 atgcaaagca gcctttcctg ctgcgcaatg gggcgaatga aggttttcac gaggccgtcg
     4141 gtgagataat gagtctctct gctgccactc caaaacactt gaaatctata ggtttgctcc
     4201 ccagtaactt ccaagaagac aatgaaacag agataaattt ccttctgaaa caagcactta
     4261 cgatagtagg caccttgcct tttacctaca tgctcgaaaa atggcgatgg atggtgttcc
     4321 aggataaaat accccgcgag cagtggacaa aaaaatggtg ggagatgaag cgagagatcg
     4381 tcggagttgt cgagcccctt ccacacgatg agacctattg cgatcctgcc agtctgttcc
     4441 acgtcagcaa tgattacagc ttcattcgat attacactcg aacgatatat cagtttcagt
     4501 ttcaagaggc gctctgccag gccgccaaac acgacggtcc acttcacaag tgtgatataa
     4561 gcaattccac cgaggcgggg caaaagcttt tgaatatgct ttcattggga aactccggac
     4621 cgtggacctt ggcattggag aatgtagtag ggagtcggaa tatggatgtc aaaccactcc
     4681 ttaattattt ccagcctctg tttgtgtggc ttaaggaaca gaacagaaat tcaactgttg
     4741 ggtggtcaac tgactggtct ccttatgcgg accagtctat aaaggtgagg ataagcctca
     4801 aatctgcgtt gggcaagaac gcctatgaat ggactgacaa cgaaatgtat ctgtttcgca
     4861 gttcagtagc ctatgctatg cgcgaatact tttcacgcga gaagaaccaa accgtcccgt
     4921 tcggcgaagc cgacgtttgg gtcagcgacc tgaagcccag ggttagtttc aatttttttg
     4981 taacttcccc taaaaatgtg agtgacatta ttccacgcag tgaggttgaa gaagcgatta
     5041 gaatgtctcg gggtcgaatt aacgacatct ttggtttgaa tgacaatagt ctggagtttt
     5101 tgggaatcta cccgacactc aagcccccgt acgagccgcc tgttacaatc tggctcatca
     5161 tatttggagt agtaatgggc acggtagttg taggaatagt aatacttatc gtgacgggaa
     5221 ttaaaggccg aaagaagaag aacgaaacga aaagagagga gaatccatac gactctatgg
     5281 acattggaaa gggcgaatca aacgctggtt tccaaaacag tgatgacgca cagacgtctt
     5341 tcggagcagc tgagcagaag ctgatcagcg aggaggacct gtaggaattc tcgaccttga
     5401 ctggcggcgc gaccttgagg cctgcgttcg cctcagttgc cccctctgtg caatggggag
     5461 acgcgcctca tcgcttgaca acggccgaag agccgccgcg cttccgtctc ccgcgtgcgc
     5521 gcgccatgct gcccaccccc gttccgcact gaccctcccc cgtgccccgc gtcccgtact
     5581 gccgccccgc cccgagtccc atgccgcagc caccgcgacg gagcccgcag gcgggaacct
     5641 gcctccgcgc gttagcgcgc acgcgcgcct catgtgtcgt ccccatcagc gccggcttcc
     5701 gtctataggc cagatgcact gtcactctgg cgaagtcgca gacccgattg gccgggacgg
     5761 aggcgcgaga ccgggttgcg ggcggggccg aacgtggtat aaaaggggcg ggaggccagg
     5821 ctcgtgccgt tttgcagacg ccaccgccga ggaaaaccgt gtactattag ccacgcgtgc
     5881 caccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     5941 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     6001 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     6061 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     6121 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     6181 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     6241 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     6301 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     6361 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     6421 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     6481 ctgagcgatc gctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6541 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6601 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     6661 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     6721 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     6781 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     6841 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     6901 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     6961 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7021 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7081 gcttaatcgc gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     7141 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     7201 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7261 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     7321 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     7381 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     7441 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     7501 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     7561 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     7621 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7681 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7741 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt catgtgagca
     7801 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7861 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7921 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7981 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8041 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8101 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8161 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8221 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8281 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8341 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8401 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8461 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8521 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8581 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8641 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8701 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8761 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8821 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8881 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8941 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9001 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9061 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9121 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9181 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9241 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9301 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9361 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9421 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9481 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9541 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9601 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     9661 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     9721 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     9781 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgct
     9841 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9901 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     9961 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
    10021 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    10081 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
    10141 gtgagg
//
