LOCUS       sequence-334964-        8160 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a PSD95:GFP fusion protein and DsRedExpress from separate 
            UAS cassettes; for long-term two-photon time-lapse imaging of 
            dendrite growth and synaptogenesis in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Niell CM, Meyer MP, Smith SJ
  TITLE     In vivo imaging of synapse formation on a growing dendritic arbor.
  JOURNAL   Nat Neurosci. 2004 Mar;7(3):254-60. Epub 2004 Feb 1.
  PUBMED    14758365
REFERENCE   2  (bases 1 to 8160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci."; date: "2004-03"; volume: "7(3)"; pages: "254-60. Epub 
            2004 Feb 1"
COMMENT     Sequence Label: 14UAS PSD95:GFP 5UAS DSRedExpress
FEATURES             Location/Qualifiers
     source          1..8160
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      134..143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             140..817
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(270..288)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(320..340)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     538..557
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     732..755
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    938..1059
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      3970..3979
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3976..3978,3979..3981,3982..4695)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3976..3978,3979..3981,3982..4692)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4021..4042)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4282..4301)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4629..4650
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4818..4939
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4946..5401)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5083..5104)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5295..5314
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5428..5532
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5534..5891
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5534..5725
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5557..5577)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5742..5877
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5926..6720
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5926..6720
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5980..5999)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6590..6609
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6908..6927)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6952..6999
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7328..7916
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7817..7836
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggagtact gtcctccggc aagggtcgac tctagagggt atataatgga tcccatcgcg
       61 tctcagcctc actttgagct cctccacacg aattctgcag tcgacggtac cgcgggcccg
      121 ggatccaccg gtcgccacca tggcctcctc cgaggacgtc atcaaggagt tcatgcgctt
      181 caaggtgcgc atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
      241 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct
      301 gcccttcgcc tgggacatcc tgtcccccca gttccagtac ggctccaagg tgtacgtgaa
      361 gcaccccgcc gacatccccg actacaagaa gctgtccttc cccgagggct tcaagtggga
      421 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
      481 ggacggctcc ttcatctaca aggtgaagtt catcggcgtg aacttcccct ccgacggccc
      541 cgtaatgcag aagaagacta tgggctggga ggcctccacc gagcgcctgt acccccgcga
      601 cggcgtgctg aagggcgaga tccacaaggc cctgaagctg aaggacggcg gccactacct
      661 ggtggagttc aagtccatct acatggccaa gaagcccgtg cagctgcccg gctactacta
      721 cgtggactcc aagctggaca tcacctccca caacgaggac tacaccatcg tggagcagta
      781 cgagcgcgcc gagggccgcc accacctgtt cctgtagcgg ccgcgactct agatcataat
      841 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
      901 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
      961 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     1021 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa gctaccggac tcagatctcg
     1081 agctcaagct taggcctcca aggcggagta ctgtcctccg ggctggcgga gtactgtcct
     1141 ccggcaaggt cggagtactg tcctccgaca ctagaggtcg gagtactgtc ctccgacgca
     1201 aggcggagta ctgtcctccg ggctgcggag tactgtcctc cggcaaggtc ggagtactgt
     1261 cctccgacac tagaggtcgg agtactgtcc tccgacgcaa ggtcggagta ctgtcctccg
     1321 acactagagg tcggagtact gtcctccgac gcaaggtcgg agtactgtcc tccgacacta
     1381 gaggtcggag tactgtcctc cgacgcaagg cggagtactg tcctccgggc tggcggagta
     1441 ctgtcctccg gcaagggtcg actctagagg gtatataatg gatcccatcg cgtctcagcc
     1501 tcactttgag ctcctccaca cgaattcgtc gccaccatgc ctctcaaacg agaagatacg
     1561 gagcgtgcgc tgcaggccat ggaagcctgt cagtctgcag gtgatgaggg tttcaggact
     1621 cgcgcagaga gacttctgac catctttcag agtgacctgt ttcaggctct gctggacatc
     1681 caggagttct atgagctgac ggtgtttgag aatcagacgg caggtagagc tctcacaccg
     1741 ggactgaagt accgttacca tgacgaggag acgccgccac tgcagcacag tcccgctcac
     1801 ctgagcacag gtaaatctgc agagatgctc cacctgggtg actctggcca cgcccccatc
     1861 gacggcatcc acgcatacac acctcagatg cacgtgtcac ctgcaaagcc agtgttactc
     1921 ccgagtggac acgctcccta ctacgcaacc tctactctga tgaacggtat ggacggagat
     1981 gttgaatatg aggagatcac gctggagagg gggaactctg ggctgggctt cagtatcgcc
     2041 ggagggactg ataaccctca tataggagac gatcccagta tcttcatcac taagatcata
     2101 ccaggaggag ctgcggcaca ggacggacga ctcagagtga acgacagcat tctgttcgtc
     2161 aatgacgtgg atgtgcggga agtcacacac agctttgcgg tggaggcgct gaaggaagca
     2221 ggaccgatcg tccggctgta cgtgctgcgc cacaaaccat cagcagagaa aatcactgag
     2281 ctcaagctca tcaaaggccc taaaggtctg ggcttcagta ttgcgggtgg tgtggggaac
     2341 cagcacgtgc ccggagacaa cagcatctac gttaccaaga tcatcgaggg cggagctgca
     2401 cataaagacg gacggctgca gatcggagac aagatcctcg ctgtgaacaa catgtatctg
     2461 gaggaggtga tgcatgaaga cgctgtagcg gcgctgaaga acactggaga cgtggtgttt
     2521 ctgagagtcg ccaaaacact ccatcagcat catcatcagg acgcctacaa ccctcctgac
     2581 atcaccagct catattctcc tcatatggac atgtctgatt acccgcaagc cctcagtccc
     2641 tcgtctccgc gccgttactc ccccatccct aaaggcctgt ttctggacga cgacatttcc
     2701 agggagccgc ggcgtgtggt gatccacaga ggctctacag gtctgggctt taatatcgtg
     2761 ggtggagaag atggagaggg aatcttcatc tccttcatac tggctggagg agctgctgat
     2821 ctgagcggag agctgaggaa aggagaccag atactgagtg tgaacggtgt agatctgcgt
     2881 cacgccactc atgaacaggc cgcggcggca ctgaagaacg caggacagac ggtcaccatc
     2941 atcacacagt acagacctga agaatacagc aggttcgagg ccaagatcca tgacctgcgg
     3001 gagcagctga tgaacagcag tttagtgtct gcagcggctt cattacggag cggaaagaga
     3061 agcttcttca tcagggctct gtttgactat gataagacgg cagacggtgg ttttctgtcg
     3121 caggccgtga gcttcaggtt cggggatgtt ctgcaggtct ttgactgcag tgatgaggaa
     3181 tggtggcagg ccggaaaact ggctccacac ggagaactgg aggagaccgg gtacattccc
     3241 agcaagagga gggtggagcg gaaggaatgg tctcgtctga agactcgagg ccgagagccc
     3301 gtcagcggac gcagcgatta catcgtgagc catgagacgg tgacgcagag tgaagtgcac
     3361 tacgcgcgtc ccgtcataat cctcggaccc agcaaggacc gtgtgaacga tgacctcctc
     3421 tccgagttcc cggacaagtt tggctcctgt gtgccccaca cgacgcggcc gaagcgcgag
     3481 tacgagatgg acggacgcga ttaccacttt gtgtcgtctc gagagcagat ggagaaggac
     3541 attcagagcc atcgcttcat cgaggcggga cagtacaaca gtcacctgta cggcaccagc
     3601 gtccagagcg tcagacaggt ggcagagcag caagggaagc actgtatcct ggatgtttca
     3661 gcgaacgcag tgcggaggct acaggccgct cagctttatc ccatcgccat ctttatacga
     3721 ccctcatcac tgcagaacgt actaaacatt agcaagcgtc tgacggagga acaggccaga
     3781 agagcgttag acagagccgt caaactggag caggacttca tcgagtgttt ctcagcgatc
     3841 gtggagggcg agagcttcga ggagatctac catcatgtga agtctgtgat cgaggagcag
     3901 tccggaccgt atagctggat cccagcacga gagagactgg gaggaggacc cgggatccac
     3961 cggccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     4021 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     4081 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     4141 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     4201 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     4261 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     4321 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     4381 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     4441 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     4501 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     4561 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     4621 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     4681 gagctgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag
     4741 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     4801 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4861 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4921 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
     4981 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     5041 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     5101 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     5161 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     5221 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     5281 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     5341 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     5401 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5461 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5521 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     5581 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5641 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5701 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     5761 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5821 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5881 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     5941 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     6001 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     6061 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     6121 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     6181 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     6241 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     6301 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     6361 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     6421 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     6481 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     6541 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     6601 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     6661 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     6721 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     6781 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     6841 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
     6901 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     6961 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     7021 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     7081 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     7141 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
     7201 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7261 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7321 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7381 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7441 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7501 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7561 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7621 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7681 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7741 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7801 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7861 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7921 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7981 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat tagttattac
     8041 tagcgctacc ggactcagat ctcgagctca agcttaggcc tccaaggcgg agtactgtcc
     8101 tccgggctgg cggagtactg tcctccggca aggtcggagt actgtcctcc gacactagag
//
