LOCUS       sequence-334965-        9473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  encodes NL63 spike protein, for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Dacon C, Tucker C, Peng L, Lee CD, Lin TH, Yuan M, Cong Y, Wang L, 
            Purser L, Williams JK, Pyo CW, Kosik I, Hu Z, Zhao M, Mohan D, 
            Cooper AJR, Peterson M, Skinner J, Dixit S, Kollins E, Huzella L, 
            Perry D, Byrum R, Lembirik S, Drawbaugh D, Eaton B, Zhang Y, Yang 
            ES, Chen M, Leung K, Weinberg RS, Pegu A, Geraghty DE, Davidson E, 
            Douagi I, Moir S, Yewdell JW, Schmaljohn C, Crompton PD, Holbrook 
            MR, Nemazee D, Mascola JR, Wilson IA, Tan J
  TITLE     Broadly neutralizing antibodies target the coronavirus fusion 
            peptide.
  JOURNAL   Science. 2022 Jul 12:eabq3773. doi: 10.1126/science.abq3773.
  PUBMED    35857439
REFERENCE   2  (bases 1 to 9473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2022 Jul 12:eabq3773. doi: 10.1126/science.abq3773."
COMMENT     Sequence Label: pcDNA3.3-NL63-D14
FEATURES             Location/Qualifiers
     source          1..9473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        8..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..591
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     542..562
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     588..612
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(4817..4836)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4861..4909
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5111..5539
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5198..5217)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5408..5429
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5548..5568)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5553..5882
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5733..5868
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5795..5814
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5949..6743
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6003..6022)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6613..6632
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6919..7040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     7010..7029
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7089..7105)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7089..7105)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7102..7124)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7113..7129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7137..7143,7144..7161,7162..7167))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7182..7203
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7320..7337)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7491..8079)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7571..7590)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8250..9041,9042..9110))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8873..8892
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9111..9215)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9290..9309)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      421 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      481 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      541 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
      601 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      661 gaccgatcca gcctccggac tgagctcctt aaggttaact ctagagaatt cggatccgcc
      721 accatgaagc tgttcctgat cctgctggtg ctgcctctgg ccagctgctt cttcacctgt
      781 aacagcaacg ccaacctgtc catgctccag ctgggcgtgc cagacaatag cagcaccatc
      841 gtgacaggcc tgctgcctac acactggttc tgcgccaatc agagcaccag cgtgtacagc
      901 gccaacggct tcttctacat cgacgtgggc aaccacagaa gcgccttcgc actgcacacc
      961 ggctactacg atgccaacca gtactacatc tacgtgacca acgagatcgg cctgaacgcc
     1021 agcgtgaccc tgaagatctg caagttcagc cggaatacca ccttcgactt tctgagcaac
     1081 gcctccagca gcttcgactg catcgtgaac ctgctgttca ccgagcagct gggagcccct
     1141 ctgggcatca caatttctgg cgaaacagtg cggctgcacc tgtacaacgt gacccggacc
     1201 ttttatgtgc cagccgccta caagctgacc aagctgagcg tgaagtgcta cttcaactac
     1261 agctgcgtgt tcagcgtggt caatgccacc gtgaccgtga acgtgaccac acacaatggc
     1321 cgggtcgtga actacaccgt gtgcgacgat tgcaacggct acaccgacaa catcttcagc
     1381 gtgcagcagg acggcagaat ccccaatggc ttccccttca acaactggtt cctgctgacc
     1441 aacggcagca ccctggttga tggcgtgtcc agactgtacc agcctctgag actgacctgc
     1501 ctgtggcctg tgcctggact gaagtctagc accggcttcg tgtacttcaa cgccaccggc
     1561 agcgacgtga actgcaatgg ctaccagcac aacagcgtgg tggacgtgat gcggtacaac
     1621 ctgaacttct ccgccaacag cctggacaat ctgaagtccg gcgtgatcgt gttcaagacc
     1681 ctccagtacg acgtgctgtt ctactgctcc aacagcagca gcggcgtgct ggacaccacc
     1741 attccttttg gccctagcag ccagccttac tactgcttca tcaactccac catcaacacc
     1801 acacacgtgt ccaccttcgt gggcatcctg cctccaaccg tgcgggaaat tgtggtggct
     1861 agaaccggcc agttttacat caacgggttc aagtacttcg acctgggctt catcgaggcc
     1921 gtgaacttta atgtgaccac cgccagcgcc accgatttct ggacagtggc ctttgccacc
     1981 tttgtggacg tgctggtcaa tgtgtccgcc accaacatcc agaatctgct gtactgcgac
     2041 agccccttcg agaagctcca gtgcgaacac ctccagttcg gcctgcaaga cggcttctac
     2101 tccgccaatt tcctggacga caacgtgctg cccgagacat atgtggccct gcctatctac
     2161 tatcagcaca ccgacatcaa tttcaccgcc acagccagct tcggcggcag ctgttacgtg
     2221 tgcaaacccc accaagtgaa catcagcctg aacggcaaca cctccgtgtg tgtgcggacc
     2281 agccacttca gcatccggta catctacaac agagtgaagt ctggcagccc tggcgacagc
     2341 tcctggcaca tctacctgaa aagcggcaca tgccccttca gcttcagcaa gctgaacaac
     2401 ttccagaagt ttaagaccat ctgcttcagc accgtggaag tgcccggcag ctgcaacttt
     2461 ccactggaag ccacctggca ctacaccagc tacacaatcg tgggcgccct gtatgtgact
     2521 tggagcgagg gcaatagcat caccggcgtg ccatatcctg tgtccggcat ccgcgagttc
     2581 agcaatctgg tgctgaacaa ttgcaccaag tacaatatct acgactacgt cggcaccggc
     2641 atcatcagaa gcagcaacca gtctctggct ggcggcatca cctacgtgtc caactctggc
     2701 aatctgctgg gctttaagaa tgtgtccacc ggcaatatct tcatcgtcac cccttgcaac
     2761 cagcctgacc aggtggcagt gtaccagcag tctatcatcg gcgccatgac cgccgtgaac
     2821 gagagcagat atggcctcca gaacctcctc cagctgccta acttctacta tgtgtccaat
     2881 ggcggcaaca actgcaccac agccgtgatg acctacagca acttcggcat ctgcgccgac
     2941 ggcagcctga ttcctgtcag acctagaaac agcagcgaca acggcatcag cgccatcatc
     3001 accgctaacc tgagcatccc cagcaactgg accacaagcg tgcaggtcga gtacctccag
     3061 atcaccagca cacccatcgt ggtcgattgc gccacctatg tgtgcaacgg caaccccaga
     3121 tgcaaaaacc tgctgaaaca gtacaccagc gcctgcaaaa ccatcgagga cgccctgaga
     3181 ctgtctgccc acctggaaac aaacgacgtg tcctccatgc tgaccttcga cagcaatgcc
     3241 ttcagcctgg ccaatgtgac ctccttcggc gactacaatc tgagcagcgt gctgccacag
     3301 cggaacatca gatccagcag aatcgccgga agaagcgccc tcgaggatct gctgtttagc
     3361 aaggtcgtga caagcggcct gggcacagtg gacgtggact acaagagctg taccaagggc
     3421 ctgtctatcg ccgatctggc ctgcgctcag tactataatg gcatcatggt gctccccggc
     3481 gtggccgatg ctgaaagaat ggccatgtac accggaagcc tgatcggcgg aatggtgctc
     3541 ggaggactga cttctgccgc cgctattccc tttagcctgg ctctccaggc cagactgaat
     3601 tacgtggccc tccagaccga tgtgctgcaa gagaaccaga agatcctggc cgccagcttc
     3661 aacaaggcca tcaacaacat cgtggccagc ttctccagcg tgaacgacgc cattacacag
     3721 accgccgagg ccatccacac agtgacaatc gccctgaaca agatccagga cgtcgtgaac
     3781 cagcagggca gcgccctgaa tcacctgaca agccagctgc ggcacaactt tcaggccatc
     3841 agcaattcca tccaggccat ctacgaccgg ctggactcta tccaggcaga tcagcaggtc
     3901 gacagactga tcaccggcag actggccgct ctgaacgcct ttgtgtccca agtgctcaac
     3961 aagtacaccg aagtgcgggg ctctagaagg ctggcccagc aaaagatcaa cgagtgcgtg
     4021 aagtcccaga gcaacagata cggcttctgc ggcaacggca cccacatctt ctctatcgtg
     4081 aacagcgccc cagacggcct cctgtttctg catacagtgc tgctgcccac cgattacaag
     4141 aacgtgaagg cttggagcgg catctgtgtg gacggcatct acggctatgt gctgagacag
     4201 cccaacctgg tgctgtactc cgataacggg gtgttcagag tgaccagcag agtgatgttc
     4261 cagcctagac tgcccgtgct gagcgacttc gtgcagatct acaactgtaa cgtgaccttc
     4321 gtgaacatct cccgcgtgga actgcacact gtgatccccg attatgtgga tgtgaacaag
     4381 accctgcaag agttcgccca gaacctgcct aaatacgtga agcccaactt cgatctgacc
     4441 cctttcaacc tgacctacct gaatctgtcc agcgaactga aacagctcga ggccaagacc
     4501 gccagcctgt ttcagaccac agtggaactc cagggcctga tcgaccagat caactctacc
     4561 tacgtggacc tgaaactgct gaaccgcttc gagaactaca tcaagtggcc ttggtgggtc
     4621 tggctgatca tctccgtggt gttcgtggtc ctgctgagcc tgctcgtgtt ctgctgtctg
     4681 tctaccggct gctgcggctg ttgcaactgt ctgaccagct ccatgagagg ctgctgtgat
     4741 tgcggctcct aactcgagcc atggaagctt gatatctaac tgactgaacc ggtggtacca
     4801 aacgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     4861 cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg
     4921 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     4981 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     5041 gcagccaacg tcggggcggc aggccctgcc atagcagatc tgcgcagctg gggctctagg
     5101 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5161 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5221 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg
     5281 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5341 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5401 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     5461 tttgatttat aagggatttt ggggatttcg gcctattggt taaaaaatga gctgatttaa
     5521 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     5581 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     5641 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5701 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     5761 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     5821 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     5881 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     5941 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     6001 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     6061 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     6121 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     6181 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     6241 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     6301 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     6361 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     6421 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     6481 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     6541 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     6601 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     6661 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     6721 gccttcttga cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg
     6781 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6841 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6901 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6961 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7021 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     7081 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7141 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7201 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7261 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7321 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7381 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7441 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7501 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7561 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7621 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7681 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7741 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7801 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7861 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7921 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7981 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8041 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8101 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8161 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8221 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8281 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8341 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8401 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8461 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8521 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8581 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8641 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8701 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8761 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8821 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8881 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8941 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9001 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9061 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9121 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9181 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9241 gacgtcgacg gatcgggaga tctcccgatc ccctatggtc gactctcagt acaatctgct
     9301 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     9361 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     9421 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tat
//
