LOCUS       sequence-334966-        4029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes NL63 spike protein, for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Dacon C, Tucker C, Peng L, Lee CD, Lin TH, Yuan M, Cong Y, Wang L, 
            Purser L, Williams JK, Pyo CW, Kosik I, Hu Z, Zhao M, Mohan D, 
            Cooper AJR, Peterson M, Skinner J, Dixit S, Kollins E, Huzella L, 
            Perry D, Byrum R, Lembirik S, Drawbaugh D, Eaton B, Zhang Y, Yang 
            ES, Chen M, Leung K, Weinberg RS, Pegu A, Geraghty DE, Davidson E, 
            Douagi I, Moir S, Yewdell JW, Schmaljohn C, Crompton PD, Holbrook 
            MR, Nemazee D, Mascola JR, Wilson IA, Tan J
  TITLE     Broadly neutralizing antibodies target the coronavirus fusion 
            peptide.
  JOURNAL   Science. 2022 Jul 12:eabq3773. doi: 10.1126/science.abq3773.
  PUBMED    35857439
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2022 Jul 12:eabq3773. doi: 10.1126/science.abq3773."
COMMENT     Sequence Label: pcDNA3.3-NL63-D14
FEATURES             Location/Qualifiers
     source          1..4029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaagctgt tcctgatcct gctggtgctg cctctggcca gctgcttctt cacctgtaac
       61 agcaacgcca acctgtccat gctccagctg ggcgtgccag acaatagcag caccatcgtg
      121 acaggcctgc tgcctacaca ctggttctgc gccaatcaga gcaccagcgt gtacagcgcc
      181 aacggcttct tctacatcga cgtgggcaac cacagaagcg ccttcgcact gcacaccggc
      241 tactacgatg ccaaccagta ctacatctac gtgaccaacg agatcggcct gaacgccagc
      301 gtgaccctga agatctgcaa gttcagccgg aataccacct tcgactttct gagcaacgcc
      361 tccagcagct tcgactgcat cgtgaacctg ctgttcaccg agcagctggg agcccctctg
      421 ggcatcacaa tttctggcga aacagtgcgg ctgcacctgt acaacgtgac ccggaccttt
      481 tatgtgccag ccgcctacaa gctgaccaag ctgagcgtga agtgctactt caactacagc
      541 tgcgtgttca gcgtggtcaa tgccaccgtg accgtgaacg tgaccacaca caatggccgg
      601 gtcgtgaact acaccgtgtg cgacgattgc aacggctaca ccgacaacat cttcagcgtg
      661 cagcaggacg gcagaatccc caatggcttc cccttcaaca actggttcct gctgaccaac
      721 ggcagcaccc tggttgatgg cgtgtccaga ctgtaccagc ctctgagact gacctgcctg
      781 tggcctgtgc ctggactgaa gtctagcacc ggcttcgtgt acttcaacgc caccggcagc
      841 gacgtgaact gcaatggcta ccagcacaac agcgtggtgg acgtgatgcg gtacaacctg
      901 aacttctccg ccaacagcct ggacaatctg aagtccggcg tgatcgtgtt caagaccctc
      961 cagtacgacg tgctgttcta ctgctccaac agcagcagcg gcgtgctgga caccaccatt
     1021 ccttttggcc ctagcagcca gccttactac tgcttcatca actccaccat caacaccaca
     1081 cacgtgtcca ccttcgtggg catcctgcct ccaaccgtgc gggaaattgt ggtggctaga
     1141 accggccagt tttacatcaa cgggttcaag tacttcgacc tgggcttcat cgaggccgtg
     1201 aactttaatg tgaccaccgc cagcgccacc gatttctgga cagtggcctt tgccaccttt
     1261 gtggacgtgc tggtcaatgt gtccgccacc aacatccaga atctgctgta ctgcgacagc
     1321 cccttcgaga agctccagtg cgaacacctc cagttcggcc tgcaagacgg cttctactcc
     1381 gccaatttcc tggacgacaa cgtgctgccc gagacatatg tggccctgcc tatctactat
     1441 cagcacaccg acatcaattt caccgccaca gccagcttcg gcggcagctg ttacgtgtgc
     1501 aaaccccacc aagtgaacat cagcctgaac ggcaacacct ccgtgtgtgt gcggaccagc
     1561 cacttcagca tccggtacat ctacaacaga gtgaagtctg gcagccctgg cgacagctcc
     1621 tggcacatct acctgaaaag cggcacatgc cccttcagct tcagcaagct gaacaacttc
     1681 cagaagttta agaccatctg cttcagcacc gtggaagtgc ccggcagctg caactttcca
     1741 ctggaagcca cctggcacta caccagctac acaatcgtgg gcgccctgta tgtgacttgg
     1801 agcgagggca atagcatcac cggcgtgcca tatcctgtgt ccggcatccg cgagttcagc
     1861 aatctggtgc tgaacaattg caccaagtac aatatctacg actacgtcgg caccggcatc
     1921 atcagaagca gcaaccagtc tctggctggc ggcatcacct acgtgtccaa ctctggcaat
     1981 ctgctgggct ttaagaatgt gtccaccggc aatatcttca tcgtcacccc ttgcaaccag
     2041 cctgaccagg tggcagtgta ccagcagtct atcatcggcg ccatgaccgc cgtgaacgag
     2101 agcagatatg gcctccagaa cctcctccag ctgcctaact tctactatgt gtccaatggc
     2161 ggcaacaact gcaccacagc cgtgatgacc tacagcaact tcggcatctg cgccgacggc
     2221 agcctgattc ctgtcagacc tagaaacagc agcgacaacg gcatcagcgc catcatcacc
     2281 gctaacctga gcatccccag caactggacc acaagcgtgc aggtcgagta cctccagatc
     2341 accagcacac ccatcgtggt cgattgcgcc acctatgtgt gcaacggcaa ccccagatgc
     2401 aaaaacctgc tgaaacagta caccagcgcc tgcaaaacca tcgaggacgc cctgagactg
     2461 tctgcccacc tggaaacaaa cgacgtgtcc tccatgctga ccttcgacag caatgccttc
     2521 agcctggcca atgtgacctc cttcggcgac tacaatctga gcagcgtgct gccacagcgg
     2581 aacatcagat ccagcagaat cgccggaaga agcgccctcg aggatctgct gtttagcaag
     2641 gtcgtgacaa gcggcctggg cacagtggac gtggactaca agagctgtac caagggcctg
     2701 tctatcgccg atctggcctg cgctcagtac tataatggca tcatggtgct ccccggcgtg
     2761 gccgatgctg aaagaatggc catgtacacc ggaagcctga tcggcggaat ggtgctcgga
     2821 ggactgactt ctgccgccgc tattcccttt agcctggctc tccaggccag actgaattac
     2881 gtggccctcc agaccgatgt gctgcaagag aaccagaaga tcctggccgc cagcttcaac
     2941 aaggccatca acaacatcgt ggccagcttc tccagcgtga acgacgccat tacacagacc
     3001 gccgaggcca tccacacagt gacaatcgcc ctgaacaaga tccaggacgt cgtgaaccag
     3061 cagggcagcg ccctgaatca cctgacaagc cagctgcggc acaactttca ggccatcagc
     3121 aattccatcc aggccatcta cgaccggctg gactctatcc aggcagatca gcaggtcgac
     3181 agactgatca ccggcagact ggccgctctg aacgcctttg tgtcccaagt gctcaacaag
     3241 tacaccgaag tgcggggctc tagaaggctg gcccagcaaa agatcaacga gtgcgtgaag
     3301 tcccagagca acagatacgg cttctgcggc aacggcaccc acatcttctc tatcgtgaac
     3361 agcgccccag acggcctcct gtttctgcat acagtgctgc tgcccaccga ttacaagaac
     3421 gtgaaggctt ggagcggcat ctgtgtggac ggcatctacg gctatgtgct gagacagccc
     3481 aacctggtgc tgtactccga taacggggtg ttcagagtga ccagcagagt gatgttccag
     3541 cctagactgc ccgtgctgag cgacttcgtg cagatctaca actgtaacgt gaccttcgtg
     3601 aacatctccc gcgtggaact gcacactgtg atccccgatt atgtggatgt gaacaagacc
     3661 ctgcaagagt tcgcccagaa cctgcctaaa tacgtgaagc ccaacttcga tctgacccct
     3721 ttcaacctga cctacctgaa tctgtccagc gaactgaaac agctcgaggc caagaccgcc
     3781 agcctgtttc agaccacagt ggaactccag ggcctgatcg accagatcaa ctctacctac
     3841 gtggacctga aactgctgaa ccgcttcgag aactacatca agtggccttg gtgggtctgg
     3901 ctgatcatct ccgtggtgtt cgtggtcctg ctgagcctgc tcgtgttctg ctgtctgtct
     3961 accggctgct gcggctgttg caactgtctg accagctcca tgagaggctg ctgtgattgc
     4021 ggctcctaa
//
