LOCUS       sequence-334986-       11092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector expressing AAVR (KIAA0319L) with a C-terminal Flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11092)
  AUTHORS   Pillay S, Meyer NL, Puschnik AS, Davulcu O, Diep J, Ishikawa Y, Jae 
            LT, Wosen JE, Nagamine CM, Chapman MS, Carette JE
  TITLE     An essential receptor for adeno-associated virus infection.
  JOURNAL   Nature. 2016 Feb 4;530(7588):108-12. doi: 10.1038/nature16465. Epub 
            2016 Jan 27.
  PUBMED    26814968
REFERENCE   2  (bases 1 to 11092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature16465"; journalName: "Nature"; date: "2016-02-4- 4"; 
            volume: "530"; issue: "7588"; pages: "108-12"
COMMENT     Sequence Label: pLenti-CMV-Puro-AAVR-FLAG
FEATURES             Location/Qualifiers
     source          1..11092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3151..3174
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3204..3792
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3257..3277)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3675..3686)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3806..4305
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3841..3864)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4238..4257
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4326..4925
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4326..4345)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4822..4842
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5063..5296
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5368..5489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5405..5424)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5459..5478
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5529..5664
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5684..5703)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5685..5703)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5713..5730)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5713..5729)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5722..5744)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5871..6326
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5958..5977)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6168..6189
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6352..6456
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6457..6525,6526..7317)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6675..6694)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7488..8076
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7977..7996
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8230..8247
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8364..8385
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8400..8405,8406..8423,8424..8430)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8438..8454
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8443..8465
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8462..8478
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8462..8478
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8497..8517
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8499..8517
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8545..8771
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8772..8952
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8999..9124
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9617..9850
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10035..10079
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10346..10462
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        10485..10788
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10789..10992
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10943..10963
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     10989..11013
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 accatggaga agaggctggg agtcaagcca aatcctgctt cctggatttt atcaggatat
       61 tattggcaga catctgcgaa gtggttgaga agcctgtacc tgttttatac ttgcttttgc
      121 ttcagcgttc tgtggttgtc aacagatgcc agtgagagca ggtgccagca ggggaagaca
      181 caatttggag ttggcctgag atctggggga gaaaatcacc tctggcttct tgaaggaacc
      241 ccctctctcc agtcatgttg ggctgcctgc tgccaggact ctgcctgcca tgtcttttgg
      301 tggctagaag ggatgtgcat tcaggcagac tgcagcaggc cccagagctg ccgggctttt
      361 aggacacact cctccaattc catgctggtg tttttaaaaa aattccaaac tgcagatgat
      421 ttgggctttc tacctgaaga tgatgtacca catcttctgg ggctaggttg gaactgggca
      481 tcttggaggc agagcccacc cagagctgca ctcagacctg ctgtatcttc cagtgaccag
      541 cagagcttaa tcaggaagct tcagaagaga ggtagtccca gtgacgtagt tacacctata
      601 gtgacacagc attctaaagt gaatgactcc aacgaattag gtggtctgac taccagtggc
      661 tctgcagagg tccacaaggc gattacaatt tccagtcccc taaccacaga cctgactgca
      721 gagctgtctg gtgggccaaa gaatgtatca gtgcaacctg aaatatcaga gggtcttgct
      781 actacgccca gcactcaaca agtaaaaagt tctgagaaaa cccagattgc tgtcccccag
      841 ccagtggctc cctcctacag ttatgctacc cctacccccc aggcctcttt ccagagcacc
      901 tcagcaccat acccagttat aaaggaactg gtggtatctg ctggagagag tgtccagata
      961 accctgccta agaatgaagt tcaattaaat gcatatgttc tccaagaacc acctaaagga
     1021 gaaacctaca cctacgactg gcagctgatt actcatccta gagactacag tggagaaatg
     1081 gaagggaaac attcccagat cctcaaacta tcgaagctca ctccaggcct gtatgaattc
     1141 aaagtgattg tagagggtca aaatgcccat ggggaaggct atgtgaacgt gacagtcaag
     1201 ccagagcccc gtaagaatcg gccccccatt gctattgtgt cacctcagtt ccaggagatc
     1261 tctttgccaa ccacttctac agtcattgat ggcagtcaaa gcactgatga tgataaaatc
     1321 gttcagtacc attgggaaga acttaagggg cctctaagag aagagaagat ttctgaagat
     1381 acagccatat taaaactaag taaactcgtc cctgggaact acactttcag cttgactgta
     1441 gtagactctg atggagctac caactctact actgcaaacc tgacagtgaa caaagctgtg
     1501 gattaccccc ctgtggccaa cgcaggcccc aaccaagtga tcaccctgcc ccaaaactcc
     1561 atcaccctct ttgggaacca gagcactgat gatcatggca tcaccagcta tgagtggtca
     1621 ctcagcccaa gcagcaaagg gaaagtggtg gagatgcagg gtgttagaac accaacctta
     1681 cagctctctg cgatgcaaga aggagactac acttaccagc tcacagtgac tgacacaata
     1741 ggacagcagg ccactgctca agtgactgtt attgtgcaac ctgaaaacaa taagcctcct
     1801 caggcagatg caggcccaga taaagagctg acccttcctg tggatagcac aaccctggat
     1861 ggcagcaaga gctcagatga tcagaaaatt atctcatatc tctgggaaaa aacacaggga
     1921 cctgatgggg tgcagctcga gaatgctaac agcagtgttg ctactgtgac tgggctgcaa
     1981 gtggggacct atgtgttcac cttgactgtc aaagatgaga ggaacctgca aagccagagc
     2041 tctgtgaatg tcattgtcaa agaagaaata aacaaaccac ctatagccaa gataactggg
     2101 aatgtggtga ttaccctacc cacgagcaca gcagagctgg atggctctaa gtcctcagat
     2161 gacaagggaa tagtcagcta cctctggact cgagatgagg ggagcccagc agcaggggag
     2221 gtgttaaatc actctgacca tcaccctatc ctttttcttt caaacctggt tgagggaacc
     2281 tacacttttc acctgaaagt gaccgatgca aagggtgaga gtgacacaga ccggaccact
     2341 gtggaggtga aacctgatcc caggaaaaac aacctggtgg agatcatctt ggatatcaac
     2401 gtcagtcagc taactgagag gctgaagggg atgttcatcc gccagattgg ggtcctcctg
     2461 ggggtgctgg attccgacat cattgtgcaa aagattcagc cgtacacgga gcagagcacc
     2521 aaaatggtat tttttgttca aaacgagcct ccccaccaga tcttcaaagg ccatgaggtg
     2581 gcagcgatgc tcaagagtga gctgcggaag caaaaggcag actttttgat attcagagcc
     2641 ttggaagtca acactgtcac atgtcagctg aactgttccg accatggcca ctgtgactcg
     2701 ttcaccaaac gctgtatctg tgaccctttt tggatggaga atttcatcaa ggtgcagctg
     2761 agggatggag acagcaactg tgagtggagc gtgttatatg ttatcattgc tacctttgtc
     2821 attgttgttg ccttgggaat cctgtcttgg actgtgatct gttgttgtaa gaggcaaaaa
     2881 ggaaaaccca agaggaaaag caagtacaag atcctggatg ccacggatca ggaaagcctg
     2941 gagctgaagc caacctcccg agcaggcatc aaacagaaag gccttttgct aagtagcagc
     3001 ctgatgcact ccgagtcaga gctggacagc gatgatgcca tctttacatg gccagaccga
     3061 gagaagggca aactcctgca tggtcagaat ggctctgtac ccaacgggca gacccctctg
     3121 aaggccagga gcccgcggga ggagatcctg gattacaagg atgacgacga taagtaaatc
     3181 cagcacagtg gcggccgctc gacaatcaac ctctggatta caaaatttgt gaaagattga
     3241 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     3301 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     3361 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     3421 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     3481 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     3541 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     3601 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     3661 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     3721 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     3781 ccgcctcccc gcctggaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     3841 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
     3901 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
     3961 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
     4021 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
     4081 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
     4141 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
     4201 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
     4261 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc
     4321 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     4381 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     4441 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     4501 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     4561 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     4621 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     4681 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     4741 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     4801 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     4861 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     4921 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat
     4981 ctcgagggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     5041 tttttaaaag aaaagggggg actggaaggg ctagctcact cccaacgaag acaagatctg
     5101 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     5161 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     5221 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     5281 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     5341 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     5401 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5461 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     5521 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5581 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     5641 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     5701 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     5761 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     5821 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     5881 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     5941 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     6001 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     6061 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     6121 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     6181 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     6241 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     6301 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     6361 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     6421 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     6481 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     6541 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     6601 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     6661 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     6721 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6781 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6841 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6901 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6961 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     7021 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     7081 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     7141 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     7201 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     7261 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     7321 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7381 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     7441 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7501 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7561 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7621 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7681 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7741 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7801 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7861 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7921 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7981 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8041 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8101 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8161 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     8221 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     8281 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     8341 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     8401 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     8461 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     8521 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     8581 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     8641 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     8701 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
     8761 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8821 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     8881 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8941 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     9001 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     9061 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     9121 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     9181 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     9241 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     9301 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     9361 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     9421 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     9481 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     9541 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     9601 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     9661 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     9721 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     9781 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     9841 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     9901 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     9961 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
    10021 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
    10081 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
    10141 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
    10201 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
    10261 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
    10321 acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg
    10381 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
    10441 aaattacaaa attcaaaatt ttatcgataa gcttgggagt tccgcgttac ataacttacg
    10501 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
    10561 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
    10621 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
    10681 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
    10741 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
    10801 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
    10861 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
    10921 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
    10981 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
    11041 gacctccata gaagacaccg actctagtcc agtgtggtgg aattctgcag at
//
