LOCUS       sequence-334987-        9331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector expressing a minimal AAVR (KIAA0319L) construct PKD1-3 with a
            C-terminal Flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Pillay S, Meyer NL, Puschnik AS, Davulcu O, Diep J, Ishikawa Y, Jae 
            LT, Wosen JE, Nagamine CM, Chapman MS, Carette JE
  TITLE     An essential receptor for adeno-associated virus infection.
  JOURNAL   Nature. 2016 Feb 4;530(7588):108-12. doi: 10.1038/nature16465. Epub 
            2016 Jan 27.
  PUBMED    26814968
REFERENCE   2  (bases 1 to 9331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature16465"; journalName: "Nature"; date: "2016-02-4- 4"; 
            volume: "530"; issue: "7588"; pages: "108-12"
COMMENT     Sequence Label: pLenti-CMV-Puro-miniAAVR-FLAG
FEATURES             Location/Qualifiers
     source          1..9331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(4..503)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(52..71)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     445..468
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    517..1105
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             623..634
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1032..1052
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1135..1158)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2627..2651)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(2648..2851)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2677..2697)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2852..3155
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    3178..3294
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(3561..3605)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3790..4023
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4516..4641
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4688..4868
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4869..5095)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(5123..5143)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5123..5141)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5162..5178)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5162..5178)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5175..5197)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5186..5202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5210..5216,5217..5234,5235..5240))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5255..5276
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5393..5410)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5564..6152)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5644..5663)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6323..7114,7115..7183))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6946..6965
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7184..7288)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7314..7769)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7451..7472)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7663..7682
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7896..7918
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7910..7927
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7911..7927
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7937..7956
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7937..7955
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7976..8111
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8151..8272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8162..8181)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8216..8235
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             8344..8577
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(8715..9314)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8798..8818)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     9295..9314
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ggtcgaaagg cccggagatg aggaagagga gaacagcgcg gcagacgtgc gcttttgaag
       61 cgtgcagaat gccgggcctc cggaggacct tcgggcgccc gccccgcccc tgagcccgcc
      121 cctgagcccg cccccggacc caccccttcc cagcctctga gcccagaaag cgaaggagca
      181 aagctgctat tggccgctgc cccaaaggcc tacccgcttc cattgctcag cggtgctgtc
      241 catctgcacg agactagtga gacgtgctac ttccatttgt cacgtcctgc acgacgcgag
      301 ctgcggggcg ggggggaact tcctgactag gggaggagta gaaggtggcg cgaaggggcc
      361 accaaagaac ggagccggtt ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag
      421 gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct
      481 tgggaaaagc gcctccccta cccggtagaa ttccaggcgg ggaggcggcc caaagggaga
      541 tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc
      601 cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc
      661 gcagaatcca ggtggcaaca caggcgagca gccatggaaa ggacgtcagc ttccccgaca
      721 acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg
      781 cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct
      841 gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca
      901 cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat
      961 acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag
     1021 cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac
     1081 aaattttgta atccagaggt tgattgtcga gcggccgcca ctgtgctgga tttacttatc
     1141 gtcgtcatcc ttgtaatcca ggatctcctc ccgcgggctc ctggccttca gaggggtctg
     1201 cccgttgggt acagagccat tctgaccatg caggagtttg cccttctctc ggtctggcca
     1261 tgtaaagatg gcatcatcgc tgtccagctc tgactcggag tgcatcaggc tgctacttag
     1321 caaaaggcct ttctgtttga tgcctgctcg ggaggttggc ttcagctcca ggctttcctg
     1381 atccgtggca tccaggatct tgtacttgct tttcctcttg ggttttcctt tttgcctctt
     1441 acaacaacag atcacagtcc aagacaggat tcccaaggca acaacaatga caaaggtagc
     1501 aatgataaca tataacacgc tccactcaca cttattgttt tcaggttgca caataacagt
     1561 cacttgagca gtggcctgct gtcctattgt gtcagtcact gtgagctggt aagtgtagtc
     1621 tccttcttgc atcgcagaga gctgtaaggt tggtgttcta acaccctgca tctccaccac
     1681 tttccctttg ctgcttgggc tgagtgacca ctcatagctg gtgatgccat gatcatcagt
     1741 gctctggttc ccaaagaggg tgatggagtt ttggggcagg gtgatcactt ggttggggcc
     1801 tgcgttggcc acaggggggt aatccacagc tttgttcact gtcaggtttg cagtagtaga
     1861 gttggtagct ccatcagagt ctactacagt caagctgaaa gtgtagttcc cagggacgag
     1921 tttacttagt tttaatatgg ctgtatcttc agaaatcttc tcttctctta gaggcccctt
     1981 aagttcttcc caatggtact gaacgatttt atcatcatca gtgctttgac tgccatcaat
     2041 gactgtagaa gtggttggca aagagatctc ctggaactga ggtgacacaa tagcaatggg
     2101 gggccgattc ttacggggct ctggcttgac tgtcacgttc acatagcctt ccccatgggc
     2161 attttgaccc tctacaatca ctttgaattc atacaggcct ggagtgagct tcgatagttt
     2221 gaggatctgg gaatgtttcc cttccatttc tccactgtag tctctaggat gagtaatcag
     2281 ctgccagtcg taggtgtagg tttctccttt aggtggttct tggagaacat atgcatttaa
     2341 ttgaacttca ttcttaggca gggttatctg gacactctct ccagcagata cctcactggc
     2401 atctgttgac aaccacagaa cgctgaagca aaagcaagta taaaacaggt acaggcttct
     2461 caaccacttc gcagatgtct gccaataata tcctgataaa atccaggaag caggatttgg
     2521 cttgactccc agcctcttct ccatggtatc tgcagaattc caccacactg gactagagtc
     2581 ggtgtcttct atggaggtca aaacagcgtg gatggcgtct ccaggcgatc tgacggttca
     2641 ctaaacgagc tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
     2701 atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac
     2761 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac
     2821 gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga
     2881 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
     2941 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg
     3001 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
     3061 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
     3121 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctcccaagct tatcgataaa
     3181 attttgaatt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg
     3241 tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt aaaagttaac
     3301 cgataccgtc gagatccgtt cactaatcga atggatctgt ctctgtctct ctctccacct
     3361 tcttcttcta ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac
     3421 gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt
     3481 cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt
     3541 gttatgttaa accaattcca caaacttgcc catttatcta attccaataa ttcttgttca
     3601 ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg
     3661 ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta
     3721 ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc
     3781 caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg
     3841 agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc
     3901 agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg taccgtcagc
     3961 gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag gaacaaagct
     4021 cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt ggtgggtgct
     4081 actcctaatg gttcaatttt tactacttta tatttatata attcacttct ccaattgtcc
     4141 ctcatatctc ctcctccagg tctgaagatc agcggccgct tgctgtgcgg tggtcttact
     4201 tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta tctctatcct
     4261 ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt cttctgatcc
     4321 tgtctgaagg gatggttgta gctgtcccag tatttgtcta cagccttctg atgtttctaa
     4381 caggccagga ttaactgcga atcgttctag ctccctgctt gcccatacta tatgttttaa
     4441 tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgcg atctaattct
     4501 cccccgctta atactgacgc tctcgcaccc atctctctcc ttctagcctc cgctagtcaa
     4561 aatttttggc gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag
     4621 ccgagtcctg cgtcgagaga gctcctctgg tttccctttc gctttcaagt ccctgttcgg
     4681 gcgccactgc tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca
     4741 gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc
     4801 ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag
     4861 agagacccgt ttatgtatcg agctaggcac ttaaatacaa tatctctgca atgcggcaat
     4921 tcagtggttc gtccaatcca tgtcagaccc gtctgttgcc ttcctaataa ggcacgatcg
     4981 taccacctta cttccaccaa tcggcatgca cggtgctttt tctctccttg taaggcatgt
     5041 tgctaactca tcgttaccat gttgcaagac tacaagagta ttgcataaga ctacattaag
     5101 cttgcagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     5161 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     5221 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     5281 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     5341 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     5401 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5461 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     5521 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     5581 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     5641 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     5701 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5761 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5821 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5881 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5941 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6001 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6061 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6121 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6181 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6241 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6301 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6361 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     6421 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     6481 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     6541 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6601 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     6661 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     6721 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6781 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6841 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     6901 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     6961 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7021 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7081 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7141 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7201 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7261 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     7321 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     7381 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     7441 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     7501 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     7561 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     7621 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     7681 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     7741 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
     7801 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     7861 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     7921 ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtacgt ccctaggcct
     7981 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
     8041 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
     8101 aggggcggga tgggcggagt taggggcggg atagctagag ccagacatga taagatacat
     8161 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     8221 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttcctctcac
     8281 tctctgatat tcatttcttt gcaagttata aatactgaat aataagatga catgaactac
     8341 tactgctaga gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt
     8401 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa
     8461 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag
     8521 acccagtaca agcaaaaagc agatcttgtc ttcgttggga gtgagctagc ccttccagtc
     8581 cccccttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta
     8641 aaggtaccgg gccctcgaga tgcatggggt cgtgcgctcc tttcggtcgg gcgctgcggg
     8701 tcgtggggcg ggcgtcaggc accgggcttg cgggtcatgc accaggtgcg cggtccttcg
     8761 ggcacctcga cgtcggcggt gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg
     8821 ggcgcggagg tctccaggaa ggcgggcacc ccggcgcgct cggccgcctc cactccgggg
     8881 agcacgacgg cgctgcccag acccttgccc tggtggtcgg gcgagacgcc gacggtggcc
     8941 aggaaccacg cgggctcctt gggccggtgc ggcgccagga ggccttccat ctgttgctgc
     9001 gcggccagcc gggaaccgct caactcggcc atgcgcgggc cgatctcggc gaacaccgcc
     9061 cccgcttcga cgctctccgg cgtggtccag accgccaccg cggcgccgtc gtccgcgacc
     9121 cacaccttgc cgatgtcgag cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc
     9181 cgctcgatgt ggcggtccgg atcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg
     9241 gcggcgaggg tgcgtacggc cctggggacg tcgtcgcggg tggcgaggcg caccgtgggc
     9301 ttgtactcgg tcatggtaag cttgggctgc a
//
