LOCUS       sequence-335000-       10332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhanced prime editing system in mammalian cells. This plasmid is 
            used by PE3 and PE3b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10332)
  AUTHORS   Liu Y, Yang G, Huang S, Li X, Wang X, Li G, Chi T, Chen Y, Huang X, 
            Wang X
  TITLE     Enhancing prime editing by Csy4-mediated processing of pegRNA.
  JOURNAL   Cell Res. 2021 Jun 8. pii: 10.1038/s41422-021-00520-x. doi: 
            10.1038/s41422-021-00520-x.
  PUBMED    34103663
REFERENCE   2  (bases 1 to 10332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2021 Jun 8. pii: 10.1038/s41422-021-00520-x. doi: 
            10.1038/s41422-021-00520-x."
COMMENT     Sequence Label: pCMV-Csy4-PE2
FEATURES             Location/Qualifiers
     source          1..10332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      652..661
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             658..1218
                     /codon_start=1
                     /product="CRISPR-associated endoribonuclease responsible 
                     for pre-crRNA processing in Pseudomonas aeruginosa 
                     (Haurwitz et al., 2010)"
                     /label=Csy4
                     /translation="MDHYLDIRLRPDPEFPPAQLMSVLFGKLHQALVAQGGDRIGVSFP
                     DLDESRSRLGERLRIHASADDLRALLARPWLEGLRDHLQFGEPAVVPHPTPYRQVSRVQ
                     AKSNPERLRRRLMRRHDLSEEEARKRIPDTVARALDLPFVTLRSQSTGQHFRLFIRHGP
                     LQVTAEEGGFTCYGLSKGGFVPWF"
     CDS             1219..1272
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1273..1293
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1294..3807,3808..3810,3811..5394)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1294..5394
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7567..7587
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7596..7613
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7636..7653)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7642..7866
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7937..7953)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7937..7953)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7950..7972)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7961..7977
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7985..7991,7992..8009,8010..8015))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8030..8051
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8168..8185)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8339..8927)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8419..8438)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9098..9889,9890..9958))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9721..9740
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9959..10063)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10142..10161)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 gaccactacc tcgacattcg cttgcgaccg gacccggaat ttcccccggc gcaactcatg
      721 agcgtgctct tcggcaagct ccaccaggcc ctggtggcac agggcgggga caggatcggc
      781 gtgagcttcc ccgacctcga cgaaagccgc tcccggctgg gcgagcgcct gcgcattcat
      841 gcctcggcgg acgaccttcg tgccctgctc gcccggccct ggctggaagg gttgcgggac
      901 catctgcaat tcggagaacc ggcagtcgtg cctcacccca caccgtaccg tcaggtcagt
      961 cgggttcagg cgaaaagcaa tccggaacgc ctgcggcggc ggctcatgcg ccggcacgat
     1021 ctgagtgagg aggaggctcg gaaacgcatt cccgatacgg tcgcgagagc cttggacctg
     1081 cccttcgtca cgctacgcag ccagagcacc ggacagcact tccgtctctt catccgccac
     1141 gggccgttgc aggtgacggc agaggaagga ggattcacct gttacgggtt gagcaaagga
     1201 ggtttcgttc cctggttcga gggcagagga agtctgttaa catgcggtga cgtcgaggag
     1261 aatcctggcc caccaaagaa gaagcggaaa gtcgacaaga agtacagcat cggcctggac
     1321 atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag
     1381 aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc
     1441 ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga
     1501 agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg
     1561 gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat
     1621 aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag
     1681 aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac
     1741 ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc
     1801 gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag
     1861 acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc
     1921 atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc
     1981 ggcgagaaga agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc
     2041 aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc
     2101 tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt
     2161 ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc
     2221 gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag
     2281 gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt
     2341 ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa
     2401 gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc
     2461 gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc
     2521 ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac
     2581 ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac
     2641 tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag
     2701 gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc
     2761 ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag
     2821 cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg
     2881 accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac
     2941 ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag
     3001 aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc
     3061 ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag
     3121 gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag
     3181 atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag
     3241 ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc
     3301 cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac
     3361 agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa
     3421 gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc
     3481 cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg
     3541 atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc
     3601 cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag
     3661 ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag
     3721 ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc
     3781 aaccggctgt ccgactacga tgtggacgct atcgtgcctc agagctttct gaaggacgac
     3841 tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg
     3901 ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag
     3961 ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa
     4021 ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac
     4081 gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc
     4141 cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc
     4201 cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac
     4261 gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga gttcgtgtac
     4321 ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc
     4381 aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt
     4441 accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg
     4501 gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc
     4561 caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc
     4621 ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag
     4681 tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa
     4741 aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa
     4801 agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg
     4861 aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg
     4921 aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc cctgccctcc
     4981 aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag
     5041 gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc
     5101 gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg
     5161 ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc
     5221 cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt tgacaccacc
     5281 atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct gatccaccag
     5341 agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg tgactctgga
     5401 ggatctagcg gaggatcctc tggcagcgag acaccaggaa caagcgagtc agcaacacca
     5461 gagagcagtg gcggcagcag cggcggcagc agcaccctaa atatagaaga tgagtatcgg
     5521 ctacatgaga cctcaaaaga gccagatgtt tctctagggt ccacatggct gtctgatttt
     5581 cctcaggcct gggcggaaac cgggggcatg ggactggcag ttcgccaagc tcctctgatc
     5641 atacctctga aagcaacctc tacccccgtg tccataaaac aataccccat gtcacaagaa
     5701 gccagactgg ggatcaagcc ccacatacag agactgttgg accagggaat actggtaccc
     5761 tgccagtccc cctggaacac gcccctgcta cccgttaaga aaccagggac taatgattat
     5821 aggcctgtcc aggatctgag agaagtcaac aagcgggtgg aagacatcca ccccaccgtg
     5881 cccaaccctt acaacctctt gagcgggctc ccaccgtccc accagtggta cactgtgctt
     5941 gatttaaagg atgccttttt ctgcctgaga ctccacccca ccagtcagcc tctcttcgcc
     6001 tttgagtgga gagatccaga gatgggaatc tcaggacaat tgacctggac cagactccca
     6061 cagggtttca aaaacagtcc caccctgttt aatgaggcac tgcacagaga cctagcagac
     6121 ttccggatcc agcacccaga cttgatcctg ctacagtacg tggatgactt actgctggcc
     6181 gccacttctg agctagactg ccaacaaggt actcgggccc tgttacaaac cctagggaac
     6241 ctcgggtatc gggcctcggc caagaaagcc caaatttgcc agaaacaggt caagtatctg
     6301 gggtatcttc taaaagaggg tcagagatgg ctgactgagg ccagaaaaga gactgtgatg
     6361 gggcagccta ctccgaagac ccctcgacaa ctaagggagt tcctagggaa ggcaggcttc
     6421 tgtcgcctct tcatccctgg gtttgcagaa atggcagccc ccctgtaccc tctcaccaaa
     6481 ccggggactc tgtttaattg gggcccagac caacaaaagg cctatcaaga aatcaagcaa
     6541 gctcttctaa ctgccccagc cctggggttg ccagatttga ctaagccctt tgaactcttt
     6601 gtcgacgaga agcagggcta cgccaaaggt gtcctaacgc aaaaactggg accttggcgt
     6661 cggccggtgg cctacctgtc caaaaagcta gacccagtag cagctgggtg gcccccttgc
     6721 ctacggatgg tagcagccat tgccgtactg acaaaggatg caggcaagct aaccatggga
     6781 cagccactag tcattctggc cccccatgca gtagaggcac tagtcaaaca accccccgac
     6841 cgctggcttt ccaacgcccg gatgactcac tatcaggcct tgcttttgga cacggaccgg
     6901 gtccagttcg gaccggtggt agccctgaac ccggctacgc tgctcccact gcctgaggaa
     6961 gggctgcaac acaactgcct tgatatcctg gccgaagccc acggaacccg acccgaccta
     7021 acggaccagc cgctcccaga cgccgaccac acctggtaca cggatggaag cagtctctta
     7081 caagagggac agcgtaaggc gggagctgcg gtgaccaccg agaccgaggt aatctgggct
     7141 aaagccctgc cagccgggac atccgctcag cgggctgaac tgatagcact cacccaggcc
     7201 ctaaagatgg cagaaggtaa gaagctaaat gtttatactg atagccgtta tgcttttgct
     7261 actgcccata tccatggaga aatatacaga aggcgtgggt ggctcacatc agaaggcaaa
     7321 gagatcaaaa ataaagacga gatcttggcc ctactaaaag ccctctttct gcccaaaaga
     7381 cttagcataa tccattgtcc aggacatcaa aagggacaca gcgccgaggc tagaggcaac
     7441 cggatggctg accaagcggc ccgaaaggca gccatcacag agactccaga cacctctacc
     7501 ctcctcatag aaaattcatc accctctggc ggctcaaaaa gaaccgccga cggcagcgaa
     7561 ttcgagccca agaagaagag gaaagtctaa ccggtcatca tcaccatcac cattgagttt
     7621 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     7681 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     7741 agaaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     7801 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     7861 ctatggcttc tgaggcggaa agaaccagct ggggctcgat accgtcgacc tctagctaga
     7921 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7981 cacacaacat acgagccgga agcataaagt gtaaagccta gggtgcctaa tgagtgagct
     8041 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8101 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8161 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8221 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8281 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8341 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8401 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8461 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8521 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8581 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8641 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8701 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8761 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8821 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8881 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8941 tcttttctac ggggtctgac actcagtgga acgaaaactc acgttaaggg attttggtca
     9001 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9061 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9121 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9181 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9241 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9301 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9361 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9421 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9481 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9541 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9601 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9661 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9721 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9781 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9841 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9901 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9961 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10021 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10081 tgccacctga cgtcgacgga tcgggagatc gatctcccga tcccctaggg tctactctca
    10141 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
    10201 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
    10261 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
    10321 gatatacgcg tt
//
