LOCUS       sequence-335003-        3291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5xGMR syntethic eye driver enhancer, requires minimal promoter 
            element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: 5xGMR
FEATURES             Location/Qualifiers
     source          1..3291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(172..190)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(173..190)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(208..224)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(208..224)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(221..243)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    232..248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(256..262,263..280,281..286))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    301..322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(439..456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(610..1198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(690..709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1369..2160,2161..2229))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1992..2011
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2230..2334)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2412..2867)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2549..2570)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2761..2780
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2993..3015
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3007..3024
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3008..3024
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3031..3050
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3031..3049
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3080..3089
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3084..3093
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcgagcccag tggaaaccct tgaaatgcct ttaagtcgag tagcagtcga ggtaggtacg
       61 tcatgcccct ccccgagacc actcatcgcc caatcactag tgaattcgcg gccgcctgca
      121 ggtcgaccat atgggagagc tcccaacgcg ttggatgcat agcttgagta ttctatagtg
      181 tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
      241 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
      301 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      361 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      421 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      481 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      541 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      601 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      661 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      721 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      781 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      841 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      901 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      961 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1021 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1081 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1141 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1201 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1261 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1321 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     1381 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     1441 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     1501 gataccgcgc gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     1561 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     1621 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     1681 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     1741 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     1801 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     1861 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     1921 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     1981 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2041 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2101 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2161 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     2221 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     2281 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     2341 tccccgaaaa gtgccacctg atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     2401 accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     2461 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2521 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2581 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgctggcc cactacgtga
     2641 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2701 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2761 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2821 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc
     2881 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     2941 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     3001 cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggc
     3061 ccgacgtcgc atgctcccgg ccgccatggc ggccgcggga attcgattgg gcgatgagtg
     3121 gtctcgggag cgtacgacta gcacctgact gtcgagccca gtggaaaccc ttgaaatgcc
     3181 tttaagtcga gcccagtgga aacccttgaa atgcctttaa gtcgagccca gtggaaaccc
     3241 ttgaaatgcc tttaagtcga gcccagtgga aacccttgaa atgcctttaa g
//
