LOCUS       sequence-335026-        5301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drosohphila vasa germline promoter and 5'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pDmVasa
FEATURES             Location/Qualifiers
     source          1..5301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..42)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(196..784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(276..295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(955..1746,1747..1815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1578..1597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1816..1920)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1998..2453)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2135..2156)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2347..2366
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2579..2601
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2593..2610
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2594..2610
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2617..2636
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2617..2635
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2666..2675
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2670..2679
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5059..5077)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5060..5077)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5095..5111)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5095..5111)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5108..5130)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5119..5135
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5143..5149,5150..5167,5168..5173))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5188..5209
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
       61 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      601 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
      841 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
      901 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
      961 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     1021 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     1081 tgcaatgata ccgcgcgacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     1141 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1201 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1261 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1321 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1381 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     1441 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     1501 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     1561 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     1621 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     1681 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     1741 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     1801 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     1861 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     1921 cacatttccc cgaaaagtgc cacctgatgc ggtgtgaaat accgcacaga tgcgtaagga
     1981 gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     2041 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     2101 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     2161 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg ctggcccact
     2221 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     2281 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     2341 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     2401 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg
     2461 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     2521 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     2581 cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
     2641 ttgggcccga cgtcgcatgc tcccggccgc catggcggcc gcgggaattc gattgggcga
     2701 tgagtggtct cgggagctgc agctggttgt aggtgcagtt gcgcttcttg cacttggcgc
     2761 acttgaggag atcggtcttg gtgccctgca cggtggccag ctgggcatcg ttgatggcct
     2821 ccttgacgaa cttctcgcgc agcttcttca tctcatcgct ggccatctcc tccggcgtca
     2881 ttttggccag ctgcttggca gtgacggcgc cgcacataaa gttgcctcgc aatccaggat
     2941 ttttggggtc cttcagattg gccacgcgtg acctaatgcg attcttgtac ttcatatccg
     3001 tgttattgaa ctcggagtaa atggcatcct ccagttcggc ggccatttcc tccggctcac
     3061 cgcatccttc gggcacttca ccaatcttca gtgcggtggc cagcatttcg cggcatttga
     3121 tgcggaccgc atctgtcatg ccgccggagg gaaacgaggt ctgtgacgag gatgtcgagc
     3181 ctttcttctc tttatccttg gatgagctgg atgaactgga cttatccttg ccggagattg
     3241 acgacgacga cttggcggca ctggtggact tcgaggcact gctgttgttg gaggagccct
     3301 ccttagcgga gctgttgttc ggtgtggttg gcgccgggct ggcgaggaat cgtttccagt
     3361 tcttgatcag cgtcttggcc agagcgatca cctcgtcgtc cttgctactc ttgcgcagct
     3421 cgtttacggt catgccgatg cgcgttttgg tcagaatgtc gagattgatg ttaagcgttt
     3481 gcagggcctt cagcaggtcc agagcctgat cctgtccctg tacggtggat atatgtatat
     3541 ttgtgacatg atgcgcggca cgttagctaa tcccgatact tactgtgccg tcgctggcca
     3601 tcttgctcat cttcttttgg attcgaaaca cttcctcctc cacgctcatt ttggcccact
     3661 taatttgatc agaggttagg ttaactggcg tcggctgtcg gctttaatcg gtgctgcggg
     3721 tctgcgacag ttgtttatgc ggaagccggg ctatttcttt ttttccctcc aataaatgtt
     3781 gtttactatt tttttcgttt ctgtattcgc agaacgagtt gacagaacac cctaacgcgt
     3841 tttagtgtta ccgggcggtg gcttgtcaag aaatgccttc ccatggctaa tacaattccc
     3901 aactggaagt agtttgggtt tcagaagtca acagtgttac cgtattttaa tgtacatctg
     3961 gcttttaagc gagtacacac acatagcatt gcgatgtttt ttaaacttgc actgcttgca
     4021 agcagggctc cactgtattt caatgaaaga acagcgtgtt gggcgttttt gaattatact
     4081 aattaccttc aataacaatt ccctatatat cacttagttt taataaataa atcgtttgag
     4141 tgtggtctag agatgatgta ttatgatgaa gtgcggttgt tggggacaag cgggatacgt
     4201 ctgcgggtca aatcggcggt gggacatagt gagagggcaa cggctccaaa accaagcgtt
     4261 gcgaacaatc accaacgcgc cctggtacgc gagaaatgtt gatatcgcac atgacctcag
     4321 catccacctt cagtttgaga acaaattaaa tcaggcacat accagacaac caggagaaca
     4381 ctgaggaggc gtcatcaaag atatctttgg acacgtggca taaacaagcc aaactattat
     4441 ctaatcaata tttttataca aattttgtat gtttccatgt tatcttcgtt gctcctgtta
     4501 gttactacgt ttcatagccc ttaaattggt tgcttaacgt aaaataaaat tatattgtat
     4561 aaaaataaag aggaattcac ctataacaaa cgaaaaagta aataaataag aacttcccca
     4621 tgttaaaaat gccaccacca tctttacata taagtatgta catatgaatg aatcacttag
     4681 gttgcttgaa tattacaatt tcattaatag ctaaatcaca tttgatgtgt tagtggaaaa
     4741 cggctatata tataaattat cgaaattgtg aatatcgaat tgcgatagca caatgggaaa
     4801 ttccaccact agatttttgg tacttttaac agatcctttt cggttttgcg ttgcgcgaag
     4861 tgatctgaac ttatcaaagt ttgtaaggta atacataaag tgaaaaagaa ttaatttgct
     4921 cttgaaaggc aggccaaatt aaaaaaaaaa tatcaaaatg cgagaccact catcgcccaa
     4981 tcactagtga attcgcggcc gcctgcaggt cgaccatatg ggagagctcc caacgcgttg
     5041 gatgcatagc ttgagtattc tatagtgtca cctaaatagc ttggcgtaat catggtcata
     5101 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     5161 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     5221 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     5281 acgcgcgggg agaggcggtt t
//
