LOCUS       sequence-335031-        5068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drosophila Polyubiquitin promoter, strongest promoter in the 
            library.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pDmPolyUbiq
FEATURES             Location/Qualifiers
     source          1..5068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..38)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(192..780)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(272..291)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(951..1742,1743..1811))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1574..1593
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1812..1916)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1994..2449)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2131..2152)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2343..2362
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2575..2597
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2589..2606
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2590..2606
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2613..2632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2613..2631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2662..2671
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2666..2675
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4822..4840)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4823..4840)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4858..4874)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4858..4874)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4871..4893)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4882..4898
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4906..4912,4913..4930,4931..4936))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4951..4972
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
       61 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      121 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      181 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      241 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      301 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      361 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      421 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      481 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
      541 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
      601 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
      661 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
      721 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
      781 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
      841 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      901 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      961 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     1021 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     1081 atgataccgc gcgacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     1141 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     1201 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     1261 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1321 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1381 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     1441 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     1501 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     1561 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     1621 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     1681 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     1741 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1801 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1861 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1921 tttccccgaa aagtgccacc tgatgcggtg tgaaataccg cacagatgcg taaggagaaa
     1981 ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     2041 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     2101 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     2161 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgctgg cccactacgt
     2221 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     2281 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     2341 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     2401 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat
     2461 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     2521 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     2581 cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg
     2641 gcccgacgtc gcatgctccc ggccgccatg gcggccgcgg gaattcgatt gggcgatgag
     2701 tggtctcggg agacatagat cttgtcgccg gaacgcagcg acagggattc caatgtgtcc
     2761 gtatccttta ggcttttgcc cttcagttcc agacgaagcg actggcgatt cgcgtgtggg
     2821 gtctgcttca gggtcttgtg aattagggcg cgcagatcgc cgatgggcgt ggcgccggag
     2881 ggcaccttca ccttgccgta cggcttgctg ttcttcgcgt tcaaaatctc cagctccatt
     2941 ttgctttcgg tgcgcttgca atcagtactg tccaaaatcg aaaatcgccg aaccgtagtg
     3001 tgaccgtgcg gggctctgcg aaaataaact tttttaggta tatggccaca cacggggaaa
     3061 gcacagtgga ttatatgtat taatatatta tccaggtttt cattacttat ccagatgtaa
     3121 gcccacttaa agcgatttaa caattatttg ccgaaagagt ataaacaaat tttacttaaa
     3181 aatggattaa gaaaagcttg tgtaagatta tgcgcagcgt tgccagatag ctccatttaa
     3241 aacacttcaa aaacaataag ttttgaaaat atatacataa atagcagtcg ttgccgcaac
     3301 gctcaacaca tcacactttt aaaacaccct ttacctacac agaattactt tttaaatttc
     3361 cagtcaagct gcgagtttca aaattatagc cggtagagaa gacagtgcta tttcaaaagc
     3421 aaactaacaa gggtcttaaa ttccaaaaca ccaatcctaa caagccttgg acttttgtaa
     3481 gtttagatca aaggtggcat tgcattcaat gtcatggtaa gaagtaggtc gtctaggtag
     3541 aaatcctcat tcagccggtc aagtcagtac gagaaaggtg tcaatttgaa attgtcttaa
     3601 aaatatttta ttgttttgta ctgtggtgag tttaaacgaa aaacacaaaa aaaaagtgat
     3661 acatagaaat cataaaaaat tttaatacaa ggtattcgta cgtatcaaaa acatttcggc
     3721 acaatttttt ttctctgtac taaagtgtta cgaacactac ggtatttttt agtgattttc
     3781 aacggacacc gaaggtatat aaacagcgtt cgcgaacggt cgccttcaaa accaattgac
     3841 atttgcagca gcaagtacaa gcagaaagta aagcgcaatc agcgaaaaat ttatacttaa
     3901 ttgttggtga ttaaagtaca attaaaagaa cattctcgaa agtcacaaga aacgtaagtt
     3961 tttaactcgc tgttaccaat tagtaataag agcaacaaga cgttgagtaa tttcaagaaa
     4021 aactgcattt caaggtcttt gttcggccat ttttttttat tcaacgctct acgtaattac
     4081 aaaataagaa attggcagcc acgcatctcg ttttcccaat gaattggcat caaaacgcaa
     4141 acaaatctat aaataaaact tacgtgttga ttttcgccaa gatttattgg caaattgtga
     4201 aattcgcagt gacgtatttg aaaattcgag aaatcacata cgcactcgag catttgtgtg
     4261 catgttatta gttagttgtt tagttaattg aagtatttta ccaacgaaat ccacttattt
     4321 ttagctgaaa tagagtaggt tgcttaaaca aagccacgtc tgaaaatttc ttattgcttg
     4381 tagttgtgac gtcaccatat acacacaaaa taatgtgtat gcatgcattt cagctgtgta
     4441 tacatacatg cacacactcg caacacgaaa acgatgacga agcaacggaa caaaggtttc
     4501 tcaactaccc tttgttccct gtttcttcgc tttcctttgt tccaatattc gtagagggtt
     4561 aataggggtt tctcaacaaa gttggcgtcg ataaataagt ttcccatttt tattccccag
     4621 ccagaaagtt agtttcaata gttttgtaat ttcaacgaaa ctcgtctgat ttcgtactaa
     4681 ttttccacat ctctattttc ttcccgcaga ataatccaaa atgcgagacc actcatcgcc
     4741 caatcactag tgaattcgcg gccgcctgca ggtcgaccat atgggagagc tcccaacgcg
     4801 ttggatgcat agcttgagta ttctatagtg tcacctaaat agcttggcgt aatcatggtc
     4861 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     4921 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     4981 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5041 ccaacgcgcg gggagaggcg gtttgcgt
//
