LOCUS       sequence-335033-       10175 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing human DNAJC6 with copGFP reporter gene,
            under control of EF1alpha promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10175)
  AUTHORS   Wulansari N, Darsono WHW, Woo HJ, Chang MY, Kim J, Bae EJ, Sun W, 
            Lee JH, Cho IJ, Shin H, Lee SJ, Lee SH
  TITLE     Neurodevelopmental defects and neurodegenerative phenotypes in human
            brain organoids carrying Parkinson's disease-linked DNAJC6 
            mutations.
  JOURNAL   Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb1540. doi: 
            10.1126/sciadv.abb1540. Print 2021 Feb.
  PUBMED    33597231
REFERENCE   2  (bases 1 to 10175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-17"; volume: "7(8). pii: 7/8/eabb1540. doi"; pages: "
            10.1126/sciadv.abb1540. Print 2021 Feb"
COMMENT     Sequence Label: pCDH-EF1a-hDNAJC6-T2A-copGFP
FEATURES             Location/Qualifiers
     source          1..10175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1966..2177
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2190..2458
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5417..5470
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5501..6163
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     misc_feature    6254..6842
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6307..6327)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6725..6736)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6916..7149
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7221..7342
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7258..7277)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7312..7331
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7382..7517
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7550..7566)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7550..7566)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7563..7585)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7574..7590
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7598..7604,7605..7622,7623..7628))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7643..7664
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7781..7798)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7952..8540)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8711..9502,9503..9571))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9334..9353
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9572..9676)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9744..9762
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9800..9822)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9922..9941
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10135..10157
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10149..10166
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10150..10166
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat
     1981 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa
     2041 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     2101 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     2161 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc
     2221 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg
     2281 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc
     2341 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga
     2401 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca
     2461 agctgtgacc ggcgcctact ctagagctag cgaattcatg agcctcctcg ggagctaccg
     2521 gaaaaagacc agcaacgatg gttatgaatc tttgcagctg gtggacagta acggggactt
     2581 aagtgcggga agcggcgggg ttggcggcaa gcagagagtg aacgccgggg cagcggcgcg
     2641 gagtcccgcc cgacagcctc cggaccgcgc cagcaccatg gacagctcag gtgcctcatc
     2701 tccagacatg gagcccagct atgggggagg tctctttgac atggtaaaag gaggtgcagg
     2761 gaggctcttt agtaacctaa aggacaactt gaaagacacc ctcaaagaca catcttctag
     2821 agtgatacaa tctgtgacca gctacacaaa gggagattta gacttcactt atgttacctc
     2881 cagaattatt gtgatgtcct ttcctctgga caatgttgac ataggattca ggaatcaggt
     2941 tgatgacatt cgaagctttt tggattccag acatcttgac cactacacag tatacaatct
     3001 gtcacctaag tcttatcgaa ctgccaagtt tcacagccgg gtctcagaat gcagttggcc
     3061 cattaggcag gctcccagtc tgcacaacct ttttgctgtg tgtcggaata tgtataactg
     3121 gctactgcag aatcccaaaa atgtctgtgt tgtccactgc ttggatggac gggcggcatc
     3181 atcaattctg gttggtgcta tgttcatttt ctgtaatctc tactctactc ctggcccagc
     3241 cattcgattg ctatatgcaa agcgaccagg aattggactt tcaccatccc ataggagata
     3301 cctgggctat atgtgtgacc tactggcaga caagccctac cgccctcact tcaagcctct
     3361 cacaattaag tcgatcactg tcagtccaat accctttttc aacaaacaga ggaatggatg
     3421 tcgcccttac tgtgatgtac tcattggaga aaccaaaata tattcgactt gcacagattt
     3481 tgaacgaatg aaagaatatc gtgtccaaga tggaaaaatc ttcattccct tgaacatcac
     3541 tgtgcaagga gacgtggttg tttccatgta tcacttgagg tcaaccattg ggagccggct
     3601 acaggctaag gtgaccaaca cacagatatt ccagcttcag tttcacactg gattcatacc
     3661 actggacaca acagttttaa agttcaccaa gcctgagtta gatgcatgtg atgtaccaga
     3721 aaaatatcct cagctatttc aggtgacact ggatgtagaa ctacagcccc atgacaaagt
     3781 aatagactta actccaccat gggaacatta ctgcacaaaa gatgtcaatc ccagcatcct
     3841 cttctcttct caccaggaac atcaagatac gctggcctta ggaggacagg ctccaataga
     3901 tatccctcca gacaacccca ggcattacgg acaaagtggt ttctttgcct ctctctgttg
     3961 gcaagatcag aaatcggaga agtcattctg tgaggaagac cacgctgccc tagtgaatca
     4021 ggaaagtgag caatcagatg atgaacttct gacactttcc agtccgcatg gcaatgccaa
     4081 tggtgacaag cctcatggag tcaagaagcc cagcaaaaag cagcaggagc cagcagcccc
     4141 tccaccccct gaggatgtgg accttttggg cctggaaggg tctgcaatga gtaacagctt
     4201 ctctccgcca gcggctcctc ccaccaattc tgaactactg agtgacctgt ttgggggtgg
     4261 aggtgcagct ggtcccaccc aggctggaca gtcaggagtg gaagatgtgt ttcatcctag
     4321 tggacctgcg tctacccagt caacaccacg ccgctctgcc acctccacct ctgcgtctcc
     4381 aaccctaaga gtgggagaag gtgccacctt tgacccattt ggagcacctt ctaaaccatc
     4441 aggtcaggat ttgctgggtt cttttctgaa cacatccagt gcttccagtg acccctttct
     4501 ccagcccaca agaagtcctt cgcccacagt acatgcttct agtacgcctg ctgtgaacat
     4561 tcagccagat gtttctggag gttgggactg gcatgctaaa ccaggaggct ttggaatggg
     4621 aagcaagtca gctgccacca gcccaaccgg atcctcgcat ggtactccca cccatcaaag
     4681 caaaccccag actctggatc cttttgccga ccttgggaca ctaggtagtt cttcctttgc
     4741 cagcaaaccc accacaccaa ctggattggg tggaggattc ccgcctctca gctcgccaca
     4801 gaaggcgtct ccccagccta tgggtggcgg gtggcagcag ggaggtgcct acaactggca
     4861 gcagccacag cctaagcctc agcccagcat gccccactcc tctccccaga accgacccaa
     4921 ctacaacgtg agcttctcag ccatgcctgg gggccagaac gaacgtggga aaggatcaag
     4981 taatttggaa gggaaacaaa aagcagctga ttttgaagac ctactctctg gtcaaggttt
     5041 caatgctcac aaagacaaaa aggggcctcg gacaatagct gagatgagaa aggaggaaat
     5101 ggccaaggaa atggatcctg agaaattaaa gattctggaa tggattgaag gcaaagaaag
     5161 aaatatcaga gcccttcttt ccacgatgca taccgtacta tgggctgggg agaccaagtg
     5221 gaaaccagtt ggcatggcag acctggtaac accagagcag gtgaagaagg tgtacaggaa
     5281 ggctgtcctg gtggtgcacc cagataaagc tactgggcaa ccctatgaac aatacgcaaa
     5341 gatgattttc atggagctca atgatgcctg gtctgaattt gaaaaccaag gccaaaagcc
     5401 cttatatgcg gccgctgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     5461 tcccggccct tccggaatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg
     5521 ccgcatcacc ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac
     5581 ccccaagcag ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag
     5641 cccctacctg ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag
     5701 cggctacgag aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat
     5761 cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg
     5821 ccgcgtgatc ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt
     5881 caccgacaag atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa
     5941 cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt
     6001 cgtggtggac agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg
     6061 gggccccatg ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat
     6121 cgtggagtac cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc
     6181 gtccaattct gccgtggacg gcaccgccgg acccggctcc accggatctc gctagagctg
     6241 aatctaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6301 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6361 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     6421 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     6481 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     6541 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     6601 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     6661 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     6721 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     6781 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     6841 gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6901 aagaaaaggg gggactggaa gggctaattc actcccaacg aagataagat ctgctttttg
     6961 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7021 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7081 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7141 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     7201 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     7261 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7321 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     7381 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7441 ttttatttat gcagaggcag aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7501 aggctttttt ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt
     7561 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7621 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7681 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7741 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7801 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7861 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7921 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7981 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8041 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8101 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8161 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8221 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8281 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8341 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     8401 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8461 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8521 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8581 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8641 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8701 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8761 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8821 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8881 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8941 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9001 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9061 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9121 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9181 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9241 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9301 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9361 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9421 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9481 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9541 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9601 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9661 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     9721 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     9781 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     9841 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     9901 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     9961 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
    10021 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    10081 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    10141 cacgacgttg taaaacgacg gccagtgcca agctg
//
