LOCUS       sequence-335034-        3727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drosphila TP10 poly(a) terminator sequence in promoter grammar, for 
            assembly of no expression control vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: Promoterless TP10
FEATURES             Location/Qualifiers
     source          1..3727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    16..37
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(154..171)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(325..913)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(405..424)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1084..1875,1876..1944))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1707..1726
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1945..2049)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2127..2582)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2264..2285)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2476..2495
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2708..2730
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2722..2739
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2723..2739
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2746..2765
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2746..2764
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2795..2804
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2799..2808
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3614..3632)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3615..3632)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3650..3666)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3650..3666)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3663..3685)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3674..3690
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3698..3704,3705..3722,3723..3727,1))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
       61 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      121 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      181 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      241 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      301 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      361 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      421 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      481 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      541 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      601 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
      661 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
      721 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
      781 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
      841 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
      901 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
      961 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1021 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1081 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     1141 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     1201 ccccagtgct gcaatgatac cgcgcgaccc acgctcaccg gctccagatt tatcagcaat
     1261 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     1321 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     1381 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     1441 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     1501 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     1561 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     1621 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     1681 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     1741 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     1801 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     1861 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     1921 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     1981 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     2041 ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat
     2101 gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc
     2161 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     2221 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     2281 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcgc
     2341 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     2401 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     2461 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     2521 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     2581 gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     2641 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     2701 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
     2761 tagggcgaat tgggcccgac gtcgcatgct cccggccgcc atggcggccg cgggaattcg
     2821 attgggcgat gagtggtctc gcattgttaa ctcgaatcgc tatccaagcc agctccattg
     2881 tcggcatcgt gctcattcta ttgacgctgg cagatttggt tttggcgcta tgggacccgt
     2941 tcggttacaa caacatgttt ccgcgcgagt ttcccgacga catgtcgcgc acgttcctga
     3001 ctgcgtactt tgagagtttc gacaacacca cgtccagaga aatcatagag tttatgcccg
     3061 agttcttttc ggaaatggtc gaaacggacg atgacgccac gtttgaatct ctatttcatt
     3121 tattagatta tgtggcatct ttagaagtta attccgacgg ccaaatgtta aacttggagg
     3181 agggtgatga aattgaggat tttgacgaat ctactttggt ggggcaagcg ttagccacta
     3241 gctcgctata cactcgcatg gagtttatgc agtacacgtt taggcaaaac acactattgt
     3301 ctatgaacaa agaaaacaac aattttaatc aaataatcat gggtttattt gcaacaaaca
     3361 caattgtggc gtttacagca tttgttatac acacagaact catatttttt atatttttcg
     3421 taatcttcct aatgatcaca ttttattaca taatcaaaga atcgtacgaa tattataaaa
     3481 caattgattt gttattttaa aaacgattca tctcccgaga ccactcatcg cccaatcact
     3541 agtgaattcg cggccgcctg caggtcgacc atatgggaga gctcccaacg cgttggatgc
     3601 atagcttgag tattctatag tgtcacctaa atagcttggc gtaatcatgg tcatagctgt
     3661 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     3721 agtgtaa
//
