LOCUS       sequence-335048-        3007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minimal viral terminator derived from Herpes Simplex 1 thymidine 
            kinase gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pHSV-TK
FEATURES             Location/Qualifiers
     source          1..3007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     35..51
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     35..51
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        67..85
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     67..84
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(220..239)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(221..239)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(246..263)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(246..262)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      404..431
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      457..912
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(544..563)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     754..775
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        990..1094
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1095..1163,1164..1955)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1313..1332)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2126..2714
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2615..2634
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2868..2885
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2900..2971)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cccgaaaggg gggccttttt tcgttttggt ccgacaggaa acagctatga ccaacggctc
       61 tatggtattt aggtgacact atagaacgaa ttcactagtg attgggcgat gagtggtctc
      121 gagcggaaca aacgacccaa cacccgtgcg ttttattctg tctttttatt gccaagccga
      181 gaccttgaga tcaatatcta aggtcctaag cggaattcac cctatagtga gtcgtattac
      241 aattcactgg ccgtcgtttt acattgagaa gagaaaagaa aaccgccgat cctgtccacc
      301 gcattactgc aaggtagtgg acaagaccgg cggtcttaag ttttttggct gaaagggtgg
      361 cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg
      421 gatggccttt ttgcgtttct acaatgttac gaatggacgc gccctgtagc ggcgcattaa
      481 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      541 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      601 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      661 aaaaacttga ttagggtgat ggttcacgta gtgggccagc gccctgatag acggtttttc
      721 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
      781 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
      841 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
      901 cgcttacaat ttcctgatgc ggtattttct acttacgcat ctgtgcggta tttcacaccg
      961 catcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     1021 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     1081 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     1141 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     1201 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     1261 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     1321 gcgcggtatt atcccgtatt gacgccgggc aagagcaact aggtcgccgc atacactatt
     1381 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     1441 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1501 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1561 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1621 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     1681 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     1741 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     1801 gtgagcgtgg gtcgcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1861 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     1921 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     1981 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     2041 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2101 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2161 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2221 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2281 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2341 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2401 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2461 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2521 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2581 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2641 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2701 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2761 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2821 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     2881 gcgaggaagc ggaaggtcag agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     2941 agtctttcga ctgagccttt cgttttattt gatgcctgct cggtaccaaa ttccagaaaa
     3001 gaggcct
//
