LOCUS       sequence-335052-        2750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bacterial terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pspy_STD
FEATURES             Location/Qualifiers
     source          1..2750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(175..194)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(176..194)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(201..218)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(201..217)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      244..699
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(331..350)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     541..562
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        777..881
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(882..950,951..1742)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1100..1119)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1913..2501
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2402..2421
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2655..2672
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2698..2714
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2698..2714
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2730..2748
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2730..2747
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gaattcacta gtgattgggc gatgagtggt ctcgagcgtt gagaagagaa aagaaaaccg
       61 ccgatcctgt ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt
      121 tggctgaaaa gccgagacct tgagatcaat atctaaggtc ctaagcggaa ttcaccctat
      181 agtgagtcgt attacaattc actggccgtc gttttacatg cgtttctaca atgttacgaa
      241 tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      301 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      361 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      421 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      481 ggccagcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      541 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      601 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      661 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttatcct
      721 tacgcatctg tgcggtattt cacaccgcat caggtggcac ttttcgggga aatgtgcgcg
      781 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      841 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
      901 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
      961 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1021 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1081 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     1141 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     1201 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     1261 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     1321 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     1381 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     1441 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     1501 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     1561 ggtttattgc tgataaatct ggagccggtg agcgtgggtc gcgcggtatc attgcagcac
     1621 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     1681 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     1741 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     1801 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     1861 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     1921 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     1981 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2041 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2101 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2161 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2221 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2281 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     2341 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2401 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2461 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     2521 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     2581 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     2641 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga aggtcatgat gcctggacag
     2701 gaaacagcta tgaccaacgg ctctatggta tttaggtgac actatagaac
//
