LOCUS       sequence-335060-        2988 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Flexible peptide linker of 4 repeats of GGS, for assembly of 
            N-terminal TL1 tags to TL3 parts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pGGSx4 NT
FEATURES             Location/Qualifiers
     source          1..2988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..21
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3..20
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(137..156)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(138..156)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(163..180)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(163..179)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      321..348
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      374..829
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(461..480)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     671..692
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        907..1011
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1012..1080,1081..1872)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1230..1249)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2043..2631
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2532..2551
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2785..2802
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2817..2888)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2959..2975
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2959..2975
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtatttaggt gacactatag aacgaattca ctagtgattg ggcgatgagt ggtctcgcac
       61 tgccgccgct acctccgctt ccaccggaac ctcccgagac cttgagatca atatctaagg
      121 tcctaagcgg aattcaccct atagtgagtc gtattacaat tcactggccg tcgttttaca
      181 ttgagaagag aaaagaaaac cgccgatcct gtccaccgca ttactgcaag gtagtggaca
      241 agaccggcgg tcttaagttt tttggctgaa agggtggcgg gcaggacgcc cgccataaac
      301 tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca
      361 atgttacgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
      421 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
      481 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
      541 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
      601 tcacgtagtg ggccagcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
      661 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
      721 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
      781 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt
      841 attttctact tacgcatctg tgcggtattt cacaccgcat caggtggcac ttttcgggga
      901 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      961 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     1021 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     1081 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     1141 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     1201 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     1261 gccgggcaag agcaactagg tcgccgcata cactattctc agaatgactt ggttgagtac
     1321 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     1381 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     1441 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     1501 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     1561 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     1621 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     1681 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc gcgcggtatc
     1741 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     1801 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1861 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1921 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     1981 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2041 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2101 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2161 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2221 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2281 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2341 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2401 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2461 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2521 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2581 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2641 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2701 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     2761 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga aggtcagaga
     2821 gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt
     2881 tttatttgat gcctgctcgg taccaaattc cagaaaagag gcctcccgaa aggggggcct
     2941 tttttcgttt tggtccgaca ggaaacagct atgaccaacg gctctatg
//
