LOCUS       sequence-335063-        2993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Flexible peptide linker of 4 repeats of GGS, for assembly of 
            C-terminal TL5 tags to TL4 parts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pGGSx4 CT
FEATURES             Location/Qualifiers
     source          1..2993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(109..128)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(110..128)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(135..152)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(135..151)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      293..320
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      346..801
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(433..452)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     643..664
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        879..983
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(984..1052,1053..1844)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1202..1221)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2015..2603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2504..2523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2757..2774
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2789..2860)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2931..2947
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2931..2947
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2963..2981
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2963..2980
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gtgattgggc gatgagtggt ctcgctcgga ggttccggtg gaagcggagg tagcggcggc
       61 agcgctcgag accttgagat caatatctaa ggtcctaagc ggaattcacc ctatagtgag
      121 tcgtattaca attcactggc cgtcgtttta cattgagaag agaaaagaaa accgccgatc
      181 ctgtccaccg cattactgca aggtagtgga caagaccggc ggtcttaagt tttttggctg
      241 aaagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc
      301 atcctgacgg atggcctttt tgcgtttcta caatgttacg aatggacgcg ccctgtagcg
      361 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
      421 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
      481 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
      541 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccagcg ccctgataga
      601 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
      661 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
      721 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
      781 aaatattaac gcttacaatt tcctgatgcg gtattttcta cttacgcatc tgtgcggtat
      841 ttcacaccgc atcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      901 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      961 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     1021 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     1081 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     1141 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     1201 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaacta ggtcgccgca
     1261 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     1321 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     1381 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     1441 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     1501 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     1561 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     1621 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     1681 ctggagccgg tgagcgtggg tcgcgcggta tcattgcagc actggggcca gatggtaagc
     1741 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     1801 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     1861 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1921 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1981 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2041 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2101 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2161 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2221 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2281 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2341 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2401 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2461 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2521 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2581 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2641 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2701 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     2761 agtcagtgag cgaggaagcg gaaggtcaga gagcgttcac cgacaaacaa cagataaaac
     2821 gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctgctc ggtaccaaat
     2881 tccagaaaag aggcctcccg aaaggggggc cttttttcgt tttggtccga caggaaacag
     2941 ctatgaccaa cggctctatg gtatttaggt gacactatag aacgaattca cta
//
